6eib - Revision history

OCA at 06:56, 29 April 2020

2020-04-29T06:56:18Z

←Older revision		Revision as of 06:56, 29 April 2020
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	==Structure of the active GGEEF domain of a diguanylate cyclase from Vibrio cholerae.==		==Structure of the active GGEEF domain of a diguanylate cyclase from Vibrio cholerae.==
-	<StructureSection load='6eib' size='340' side='right' caption='[[6eib]], [[Resolution\|resolution]] 1.94Å' scene=''>	+	<StructureSection load='6eib' size='340' side='right'caption='[[6eib]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6eib]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EIB OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6EIB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6eib]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibc3 Vibc3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EIB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6EIB FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6eib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eib OCA], [http://pdbe.org/6eib PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eib RCSB], [http://www.ebi.ac.uk/pdbsum/6eib PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eib ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VC0395_0300 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=345073 VIBC3])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6eib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eib OCA], [http://pdbe.org/6eib PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eib RCSB], [http://www.ebi.ac.uk/pdbsum/6eib PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eib ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cyclic-di-GMP (c-di-GMP) synthesized by diguanylate cyclases has been an important and ubiquitous secondary messenger in almost all bacterial systems. In Vibrio cholerae, c-di-GMP plays an intricate role in the production of the exopolysaccharide matrix, and thereby, in biofilm formation. The formation of the surface biofilm enables the bacteria to survive in aquatic bodies, when not infecting a human host. Diguanylate cyclases are the class of enzymes which synthesize c-di-GMP from two molecules of GTP and are endowed with a GGDEF or, a GGEEF signature domain. The VC0395_0300 protein from V. cholerae, has been established as a diguanylate cyclase with a necessary role in biofilm formation. Here we present the structure of an N-terminally truncated form of VC0395_0300, which retains the active GGEEF domain for diguanylate cyclase activity but lacks 160 residues from the poorly organized N-terminal domain. X-ray diffraction data was collected from a crystal of VC0395_0300(161-321) to a resolution of 1.9 A. The structure displays remarkable topological similarity with diguanylate cyclases from other bacterial systems, but lacks the binding site for c-di-GMP present in its homologues. Finally, we demonstrate the ability of the truncated diguanylate cyclase VC0395_0300(161-321) to produce c-di-GMP, and its role in biofilm formation for the bacteria.
		+
		+	Structure of the active GGEEF domain of a diguanylate cyclase from Vibrio cholerae.,Chouhan OP, Roske Y, Heinemann U, Biswas S Biochem Biophys Res Commun. 2020 Mar 5;523(2):287-292. doi:, 10.1016/j.bbrc.2019.11.179. Epub 2019 Dec 18. PMID:31862141<ref>PMID:31862141</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6eib" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Vibc3]]
	[[Category: Chouhan, O P]]		[[Category: Chouhan, O P]]
	[[Category: Roske, Y]]		[[Category: Roske, Y]]

OCA at 08:09, 10 October 2018

2018-10-10T08:09:14Z

←Older revision		Revision as of 08:09, 10 October 2018
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-	'''Unreleased structure'''

-	~~The entry 6eib is ON HOLD~~	+	==Structure of the active GGEEF domain of a diguanylate cyclase from Vibrio cholerae.==
-		+	<StructureSection load='6eib' size='340' side='right' caption='[[6eib]], [[Resolution\|resolution]] 1.94Å' scene=''>
-	~~Authors: Chouhan, O.P., Roske, Y.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6eib]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EIB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EIB FirstGlance]. <br>
-	~~Description:~~ Structure of the active GGEEF domain of a diguanylate cyclase from Vibrio cholerae.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6eib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eib OCA], [http://pdbe.org/6eib PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eib RCSB], [http://www.ebi.ac.uk/pdbsum/6eib PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eib ProSAT]</span></td></tr>
-	[[Category: Chouhan, O.P]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chouhan, O P]]
	[[Category: Roske, Y]]		[[Category: Roske, Y]]
		+	[[Category: Diguanylate cyclase]]
		+	[[Category: Ggeef domain]]
		+	[[Category: Transferase]]

OCA: Protected "6eib" [edit=sysop:move=sysop]

2017-09-27T17:12:36Z

Protected "6eib" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:12, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 6eib is ON HOLD Authors: Chouhan, O.P., Roske, Y. Description: Structure of the active GGEEF domain of a diguanylate cyclase from Vibrio cholerae. ...

2017-09-27T17:12:35Z

New page: '''Unreleased structure''' The entry 6eib is ON HOLD Authors: Chouhan, O.P., Roske, Y. Description: Structure of the active GGEEF domain of a diguanylate cyclase from Vibrio cholerae. ...

New page

'''Unreleased structure'''

The entry 6eib is ON HOLD

Authors: Chouhan, O.P., Roske, Y.

Description: Structure of the active GGEEF domain of a diguanylate cyclase from Vibrio cholerae.
[[Category: Unreleased Structures]]
[[Category: Chouhan, O.P]]
[[Category: Roske, Y]]