6eji - Revision history

OCA at 07:08, 15 February 2018

2018-02-15T07:08:32Z

←Older revision		Revision as of 07:08, 15 February 2018
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	<StructureSection load='6eji' size='340' side='right' caption='[[6eji]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='6eji' size='340' side='right' caption='[[6eji]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6eji]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EJI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EJI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6eji]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"campylobacter_fetus_subsp._jejuni"_smibert_1974 "campylobacter fetus subsp. jejuni" smibert 1974]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EJI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EJI FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=UD2:URIDINE-DIPHOSPHATE-N-ACETYLGALACTOSAMINE'>UD2</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=UD2:URIDINE-DIPHOSPHATE-N-ACETYLGALACTOSAMINE'>UD2</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">wlaC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197 "Campylobacter fetus subsp. jejuni" Smibert 1974])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6eji FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eji OCA], [http://pdbe.org/6eji PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eji RCSB], [http://www.ebi.ac.uk/pdbsum/6eji PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eji ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6eji FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eji OCA], [http://pdbe.org/6eji PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eji RCSB], [http://www.ebi.ac.uk/pdbsum/6eji PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eji ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation. Using an unknown counting mechanism, PglH catalyzes the transfer of exactly three alpha1,4 N-acetylgalactosamine (GalNAc) units to the growing LLO precursor, GalNAc-alpha1,4-GalNAc-alpha1,3-Bac-alpha1-PP-undecaprenyl. Here, we present crystal structures of PglH in three distinct states, including a binary complex with UDP-GalNAc and two ternary complexes containing a chemo-enzymatically generated LLO analog and either UDP or synthetic, nonhydrolyzable UDP-CH2-GalNAc. PglH contains an amphipathic helix ("ruler helix") that has a dual role of facilitating membrane attachment and glycan counting. The ruler helix contains three positively charged side chains that can bind the pyrophosphate group of the LLO substrate and thus limit the addition of GalNAc units to three. These results, combined with molecular dynamics simulations, provide the mechanism of glycan counting by PglH.
		+
		+	Structural basis of the molecular ruler mechanism of a bacterial glycosyltransferase.,Ramirez AS, Boilevin J, Mehdipour AR, Hummer G, Darbre T, Reymond JL, Locher KP Nat Commun. 2018 Jan 31;9(1):445. doi: 10.1038/s41467-018-02880-2. PMID:29386647<ref>PMID:29386647</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6eji" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Campylobacter fetus subsp. jejuni smibert 1974]]
	[[Category: Boilevin, J]]		[[Category: Boilevin, J]]
	[[Category: Darbre, T]]		[[Category: Darbre, T]]

OCA at 06:21, 7 February 2018

2018-02-07T06:21:07Z

←Older revision		Revision as of 06:21, 7 February 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6eji is ~~ON HOLD until Paper Publication~~	+	==Structure of a glycosyltransferase==
-		+	<StructureSection load='6eji' size='340' side='right' caption='[[6eji]], [[Resolution\|resolution]] 2.30Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6eji]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EJI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EJI FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=UD2:URIDINE-DIPHOSPHATE-N-ACETYLGALACTOSAMINE'>UD2</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6eji FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eji OCA], [http://pdbe.org/6eji PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eji RCSB], [http://www.ebi.ac.uk/pdbsum/6eji PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eji ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Boilevin, J]]
		+	[[Category: Darbre, T]]
		+	[[Category: Hummer, G]]
		+	[[Category: Locher, K P]]
		+	[[Category: Mehdipour, A R]]
		+	[[Category: Ramirez, A S]]
		+	[[Category: Reymond, J L]]
		+	[[Category: Glycosyltransferase]]
		+	[[Category: Transferase]]

OCA at 08:16, 4 October 2017

2017-10-04T08:16:43Z

←Older revision		Revision as of 08:16, 4 October 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6eji is ON HOLD	+	The entry 6eji is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6eji" [edit=sysop:move=sysop]

2017-09-27T17:14:58Z

Protected "6eji" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:14, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 6eji is ON HOLD Authors: Description: Category: Unreleased Structures

2017-09-27T17:14:57Z

New page: '''Unreleased structure''' The entry 6eji is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6eji is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]