6ek4 - Revision history

OCA at 07:31, 1 May 2024

2024-05-01T07:31:08Z

←Older revision		Revision as of 07:31, 1 May 2024
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	<StructureSection load='6ek4' size='340' side='right'caption='[[6ek4]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='6ek4' size='340' side='right'caption='[[6ek4]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ek4]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EK4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6EK4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ek4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Photorhabdus_luminescens Photorhabdus luminescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EK4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6EK4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6ek4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ek4 OCA], [~~http~~://pdbe.org/6ek4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6ek4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6ek4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6ek4 ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ek4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ek4 OCA], [https://pdbe.org/6ek4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ek4 RCSB], [https://www.ebi.ac.uk/pdbsum/6ek4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ek4 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/A0A1B8YJW8_9GAMM A0A1B8YJW8_9GAMM]
-	Pore-forming toxins (PFT) are virulence factors that transform from soluble to membrane-bound states. The Yersinia YaxAB system represents a family of binary alpha-PFTs with orthologues in human, insect, and plant pathogens, with unknown structures. YaxAB was shown to be cytotoxic and likely involved in pathogenesis, though the molecular basis for its two-component lytic mechanism remains elusive. Here, we present crystal structures of YaxA and YaxB, together with a cryo-electron microscopy map of the YaxAB complex. Our structures reveal a pore predominantly composed of decamers of YaxA-YaxB heterodimers. Both subunits bear membrane-active moieties, but only YaxA is capable of binding to membranes by itself. YaxB can subsequently be recruited to membrane-associated YaxA and induced to present its lytic transmembrane helices. Pore formation can progress by further oligomerization of YaxA-YaxB dimers. Our results allow for a comparison between pore assemblies belonging to the wider ClyA-like family of alpha-PFTs, highlighting diverse pore architectures.	+
-		+
-	Structure and mechanism of the two-component alpha-helical pore-forming toxin YaxAB.,Brauning B, Bertosin E, Praetorius F, Ihling C, Schatt A, Adler A, Richter K, Sinz A, Dietz H, Groll M Nat Commun. 2018 May 4;9(1):~~1806. doi: 10.1038~~/~~s41467-018-04139-2. PMID:29728606<ref>PMID:29728606<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 6ek4" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Braeuning, B~~]]	+	[[Category: Photorhabdus luminescens]]
-	[[Category: ~~Groll, M~~]]	+	[[Category: Braeuning B]]
-	[[Category: ~~Adventitious membrane protein]]~~	+	[[Category: Groll M]]
-	~~[[Category: Alpha-helical]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Pathogen]]~~	+
-	~~[[Category: Pore forming toxin~~]]	+

OCA at 09:11, 11 November 2020

2020-11-11T09:11:33Z

←Older revision		Revision as of 09:11, 11 November 2020
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	==PaxB from Photorhabdus luminescens==		==PaxB from Photorhabdus luminescens==
-	<StructureSection load='6ek4' size='340' side='right' caption='[[6ek4]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='6ek4' size='340' side='right'caption='[[6ek4]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ek4]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EK4 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6EK4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ek4]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EK4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6EK4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ek4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ek4 OCA], [http://pdbe.org/6ek4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ek4 RCSB], [http://www.ebi.ac.uk/pdbsum/6ek4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ek4 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ek4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ek4 OCA], [http://pdbe.org/6ek4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ek4 RCSB], [http://www.ebi.ac.uk/pdbsum/6ek4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ek4 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Braeuning, B]]		[[Category: Braeuning, B]]
	[[Category: Groll, M]]		[[Category: Groll, M]]

OCA at 05:34, 16 May 2018

2018-05-16T05:34:09Z

←Older revision		Revision as of 05:34, 16 May 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6ek4 is ~~ON HOLD until Paper~~ Publication	+	==PaxB from Photorhabdus luminescens==
		+	<StructureSection load='6ek4' size='340' side='right' caption='[[6ek4]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6ek4]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EK4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EK4 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ek4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ek4 OCA], [http://pdbe.org/6ek4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ek4 RCSB], [http://www.ebi.ac.uk/pdbsum/6ek4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ek4 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Pore-forming toxins (PFT) are virulence factors that transform from soluble to membrane-bound states. The Yersinia YaxAB system represents a family of binary alpha-PFTs with orthologues in human, insect, and plant pathogens, with unknown structures. YaxAB was shown to be cytotoxic and likely involved in pathogenesis, though the molecular basis for its two-component lytic mechanism remains elusive. Here, we present crystal structures of YaxA and YaxB, together with a cryo-electron microscopy map of the YaxAB complex. Our structures reveal a pore predominantly composed of decamers of YaxA-YaxB heterodimers. Both subunits bear membrane-active moieties, but only YaxA is capable of binding to membranes by itself. YaxB can subsequently be recruited to membrane-associated YaxA and induced to present its lytic transmembrane helices. Pore formation can progress by further oligomerization of YaxA-YaxB dimers. Our results allow for a comparison between pore assemblies belonging to the wider ClyA-like family of alpha-PFTs, highlighting diverse pore architectures.

-	~~Authors: Braeuning~~, B., ~~Groll~~, M.	+	Structure and mechanism of the two-component alpha-helical pore-forming toxin YaxAB.,Brauning B, Bertosin E, Praetorius F, Ihling C, Schatt A, Adler A, Richter K, Sinz A, Dietz H, Groll M Nat Commun. 2018 May 4;9(1):1806. doi: 10.1038/s41467-018-04139-2. PMID:29728606<ref>PMID:29728606</ref>

-	~~Description: PaxB from Photorhabdus luminescens~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 6ek4" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Braeuning, B]]		[[Category: Braeuning, B]]
	[[Category: Groll, M]]		[[Category: Groll, M]]
		+	[[Category: Adventitious membrane protein]]
		+	[[Category: Alpha-helical]]
		+	[[Category: Membrane protein]]
		+	[[Category: Pathogen]]
		+	[[Category: Pore forming toxin]]

OCA: Protected "6ek4" [edit=sysop:move=sysop]

2017-10-04T08:17:57Z

Protected "6ek4" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:17, 4 October 2017

OCA: New page: '''Unreleased structure''' The entry 6ek4 is ON HOLD until Paper Publication Authors: Braeuning, B., Groll, M. Description: PaxB from Photorhabdus luminescens [[Category: Unreleased St...

2017-10-04T08:17:56Z

New page: '''Unreleased structure''' The entry 6ek4 is ON HOLD until Paper Publication Authors: Braeuning, B., Groll, M. Description: PaxB from Photorhabdus luminescens [[Category: Unreleased St...

New page

'''Unreleased structure'''

The entry 6ek4 is ON HOLD until Paper Publication

Authors: Braeuning, B., Groll, M.

Description: PaxB from Photorhabdus luminescens
[[Category: Unreleased Structures]]
[[Category: Braeuning, B]]
[[Category: Groll, M]]