6erb - Revision history

OCA at 10:20, 18 March 2021

2021-03-18T10:20:16Z

←Older revision		Revision as of 10:20, 18 March 2021
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	<StructureSection load='6erb' size='340' side='right'caption='[[6erb]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='6erb' size='340' side='right'caption='[[6erb]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6erb]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ERB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ERB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6erb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ERB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ERB FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">~~A6B47_14470~~ ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1351 "Enterococcus proteiformis" Thiercelin and Jouhaud 1903])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">D350_01176 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1351 "Enterococcus proteiformis" Thiercelin and Jouhaud 1903])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6erb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6erb OCA], [~~http~~://pdbe.org/6erb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6erb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6erb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6erb ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6erb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6erb OCA], [https://pdbe.org/6erb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6erb RCSB], [https://www.ebi.ac.uk/pdbsum/6erb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6erb ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 6erb" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6erb" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Fic protein 3D structures\|Fic protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:22, 20 March 2019

2019-03-20T08:22:43Z

←Older revision		Revision as of 08:22, 20 March 2019
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	==Enterococcus faecalis FIC protein (H111A) in complex with sulfate.==		==Enterococcus faecalis FIC protein (H111A) in complex with sulfate.==
-	<StructureSection load='6erb' size='340' side='right' caption='[[6erb]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='6erb' size='340' side='right'caption='[[6erb]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6erb]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ERB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ERB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6erb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ERB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ERB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A6B47_14470 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1351 "Enterococcus proteiformis" Thiercelin and Jouhaud 1903])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6erb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6erb OCA], [http://pdbe.org/6erb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6erb RCSB], [http://www.ebi.ac.uk/pdbsum/6erb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6erb ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6erb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6erb OCA], [http://pdbe.org/6erb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6erb RCSB], [http://www.ebi.ac.uk/pdbsum/6erb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6erb ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	FIC proteins regulate molecular processes from bacteria to humans by catalyzing post-translational modifications (PTM), the most frequent being the addition of AMP or AMPylation. In many AMPylating FIC proteins, a structurally conserved glutamate represses AMPylation and, in mammalian FICD, also supports deAMPylation of BiP/GRP78, a key chaperone of the unfolded protein response. Currently, a direct signal regulating these FIC proteins has not been identified. Here, we use X-ray crystallography and in vitro PTM assays to address this question. We discover that Enterococcus faecalis FIC (EfFIC) catalyzes both AMPylation and deAMPylation and that the glutamate implements a multi-position metal switch whereby Mg(2+) and Ca(2+) control AMPylation and deAMPylation differentially without a conformational change. Remarkably, Ca(2+) concentration also tunes deAMPylation of BiP by human FICD. Our results suggest that the conserved glutamate is a signature of AMPylation/deAMPylation FIC bifunctionality and identify metal ions as diffusible signals that regulate such FIC proteins directly.
		+
		+	A Ca(2+)-regulated deAMPylation switch in human and bacterial FIC proteins.,Veyron S, Oliva G, Rolando M, Buchrieser C, Peyroche G, Cherfils J Nat Commun. 2019 Mar 8;10(1):1142. doi: 10.1038/s41467-019-09023-1. PMID:30850593<ref>PMID:30850593</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6erb" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Enterococcus proteiformis thiercelin and jouhaud 1903]]
		+	[[Category: Large Structures]]
	[[Category: Cherfils, J]]		[[Category: Cherfils, J]]
	[[Category: Veyron, S]]		[[Category: Veyron, S]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA at 09:47, 13 February 2019

2019-02-13T09:47:23Z

←Older revision		Revision as of 09:47, 13 February 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6erb is ~~ON HOLD until Paper Publication~~	+	==Enterococcus faecalis FIC protein (H111A) in complex with sulfate.==
-		+	<StructureSection load='6erb' size='340' side='right' caption='[[6erb]], [[Resolution\|resolution]] 2.20Å' scene=''>
-	~~Authors~~: ~~Veyron~~, S., ~~Cherfils~~, J.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6erb]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ERB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ERB FirstGlance]. <br>
-	~~Description: Mutant toxin from Enterococcus faecalis in complex with sulfate~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6erb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6erb OCA], [http://pdbe.org/6erb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6erb RCSB], [http://www.ebi.ac.uk/pdbsum/6erb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6erb ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cherfils, J]]
	[[Category: Veyron, S]]		[[Category: Veyron, S]]
-	[[Category: ~~Cherfils, J~~]]	+	[[Category: Toxin]]

OCA at 06:07, 26 September 2018

2018-09-26T06:07:22Z

←Older revision		Revision as of 06:07, 26 September 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6erb is ON HOLD	+	The entry 6erb is ON HOLD until Paper Publication

	Authors: Veyron, S., Cherfils, J.		Authors: Veyron, S., Cherfils, J.

OCA: Protected "6erb" [edit=sysop:move=sysop]

2017-10-25T06:02:10Z

Protected "6erb" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:02, 25 October 2017

OCA: New page: '''Unreleased structure''' The entry 6erb is ON HOLD Authors: Veyron, S., Cherfils, J. Description: Mutant toxin from Enterococcus faecalis in complex with sulfate [[Category: Unreleas...

2017-10-25T06:02:09Z

New page: '''Unreleased structure''' The entry 6erb is ON HOLD Authors: Veyron, S., Cherfils, J. Description: Mutant toxin from Enterococcus faecalis in complex with sulfate [[Category: Unreleas...

New page

'''Unreleased structure'''

The entry 6erb is ON HOLD

Authors: Veyron, S., Cherfils, J.

Description: Mutant toxin from Enterococcus faecalis in complex with sulfate
[[Category: Unreleased Structures]]
[[Category: Veyron, S]]
[[Category: Cherfils, J]]