6esq - Revision history

OCA at 12:21, 9 May 2024

2024-05-09T12:21:13Z

←Older revision		Revision as of 12:21, 9 May 2024
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	<StructureSection load='6esq' size='340' side='right'caption='[[6esq]], [[Resolution\|resolution]] 2.95Å' scene=''>		<StructureSection load='6esq' size='340' side='right'caption='[[6esq]], [[Resolution\|resolution]] 2.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6esq]] is a 12 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Methanothermococcus_thermolithotrophicus Methanothermococcus thermolithotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ESQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6ESQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6esq]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermococcus_thermolithotrophicus Methanothermococcus thermolithotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ESQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ESQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.95Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetyl-CoA_C-acetyltransferase Acetyl-CoA C-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.9 2.3.1.9] </span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6esq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6esq OCA], [~~http~~://pdbe.org/6esq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6esq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6esq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6esq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6esq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6esq OCA], [https://pdbe.org/6esq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6esq RCSB], [https://www.ebi.ac.uk/pdbsum/6esq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6esq ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A384E138_METTL A0A384E138_METTL]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Acetyl-CoA C-acetyltransferase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Methanothermococcus thermolithotrophicus]]		[[Category: Methanothermococcus thermolithotrophicus]]
-	[[Category: Engilberge, S]]	+	[[Category: Engilberge S]]
-	[[Category: Erb~~, J T~~]]	+	[[Category: Erb JT]]
-	[[Category: Girard, E]]	+	[[Category: Girard E]]
-	[[Category: Maury, O]]	+	[[Category: Maury O]]
-	[[Category: Riobe, F]]	+	[[Category: Riobe F]]
-	[[Category: Shima, S]]	+	[[Category: Shima S]]
-	[[Category: Voegeli, B]]	+	[[Category: Voegeli B]]
-	[[Category: Wagner, T]]	+	[[Category: Wagner T]]
-	~~[[Category: Archaea]]~~	+
-	~~[[Category: Duf35 family]]~~	+
-	~~[[Category: Enzymatic channeling]]~~	+
-	~~[[Category: Isoprenoid synthesis]]~~	+
-	~~[[Category: Lipid biosynthesis]]~~	+
-	~~[[Category: Mevalonate production]]~~	+
-	~~[[Category: Multi-enzymatic complex]]~~	+
-	~~[[Category: Scaffold protein]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 09:14, 11 November 2020

2020-11-11T09:14:41Z

←Older revision		Revision as of 09:14, 11 November 2020
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	==Structure of the acetoacetyl-CoA thiolase/HMG-CoA synthase complex from Methanothermococcus thermolithotrophicus soaked with acetyl-CoA==		==Structure of the acetoacetyl-CoA thiolase/HMG-CoA synthase complex from Methanothermococcus thermolithotrophicus soaked with acetyl-CoA==
-	<StructureSection load='6esq' size='340' side='right' caption='[[6esq]], [[Resolution\|resolution]] 2.95Å' scene=''>	+	<StructureSection load='6esq' size='340' side='right'caption='[[6esq]], [[Resolution\|resolution]] 2.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6esq]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanothermococcus_thermolithotrophicus Methanothermococcus thermolithotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ESQ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ESQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6esq]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanothermococcus_thermolithotrophicus Methanothermococcus thermolithotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ESQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ESQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetyl-CoA_C-acetyltransferase Acetyl-CoA C-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.9 2.3.1.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetyl-CoA_C-acetyltransferase Acetyl-CoA C-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.9 2.3.1.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6esq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6esq OCA], [http://pdbe.org/6esq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6esq RCSB], [http://www.ebi.ac.uk/pdbsum/6esq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6esq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6esq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6esq OCA], [http://pdbe.org/6esq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6esq RCSB], [http://www.ebi.ac.uk/pdbsum/6esq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6esq ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 6esq" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6esq" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Thiolase 3D structures\|Thiolase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Acetyl-CoA C-acetyltransferase]]		[[Category: Acetyl-CoA C-acetyltransferase]]
		+	[[Category: Large Structures]]
	[[Category: Methanothermococcus thermolithotrophicus]]		[[Category: Methanothermococcus thermolithotrophicus]]
	[[Category: Engilberge, S]]		[[Category: Engilberge, S]]

OCA at 08:05, 21 March 2018

2018-03-21T08:05:46Z

←Older revision		Revision as of 08:05, 21 March 2018
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6esq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6esq OCA], [http://pdbe.org/6esq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6esq RCSB], [http://www.ebi.ac.uk/pdbsum/6esq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6esq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6esq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6esq OCA], [http://pdbe.org/6esq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6esq RCSB], [http://www.ebi.ac.uk/pdbsum/6esq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6esq ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Many reactions within a cell are thermodynamically unfavorable. To efficiently run some of those endergonic reactions, nature evolved intermediate-channeling enzyme complexes, in which the products of the first endergonic reactions are immediately consumed by the second exergonic reactions. Based on this concept, we studied how archaea overcome the unfavorable first reaction of isoprenoid biosynthesis-the condensation of two molecules of acetyl-CoA to acetoacetyl-CoA catalyzed by acetoacetyl-CoA thiolases (thiolases). We natively isolated an enzyme complex comprising the thiolase and 3-hydroxy-3-methylglutaryl (HMG)-CoA synthase (HMGCS) from a fast-growing methanogenic archaeon, Methanothermococcus thermolithotrophicus HMGCS catalyzes the second reaction in the mevalonate pathway-the exergonic condensation of acetoacetyl-CoA and acetyl-CoA to HMG-CoA. The 380-kDa crystal structure revealed that both enzymes are held together by a third protein (DUF35) with so-far-unknown function. The active-site clefts of thiolase and HMGCS form a fused CoA-binding site, which allows for efficient coupling of the endergonic thiolase reaction with the exergonic HMGCS reaction. The tripartite complex is found in almost all archaeal genomes and in some bacterial ones. In addition, the DUF35 proteins are also important for polyhydroxyalkanoate (PHA) biosynthesis, most probably by functioning as a scaffold protein that connects thiolase with 3-ketoacyl-CoA reductase. This natural and highly conserved enzyme complex offers great potential to improve isoprenoid and PHA biosynthesis in biotechnologically relevant organisms.
		+
		+	Archaeal acetoacetyl-CoA thiolase/HMG-CoA synthase complex channels the intermediate via a fused CoA-binding site.,Vogeli B, Engilberge S, Girard E, Riobe F, Maury O, Erb TJ, Shima S, Wagner T Proc Natl Acad Sci U S A. 2018 Mar 12. pii: 1718649115. doi:, 10.1073/pnas.1718649115. PMID:29531083<ref>PMID:29531083</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6esq" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:33, 14 March 2018

2018-03-14T06:33:12Z

←Older revision		Revision as of 06:33, 14 March 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6esq is ~~ON HOLD~~	+	==Structure of the acetoacetyl-CoA thiolase/HMG-CoA synthase complex from Methanothermococcus thermolithotrophicus soaked with acetyl-CoA==
-		+	<StructureSection load='6esq' size='340' side='right' caption='[[6esq]], [[Resolution\|resolution]] 2.95Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6esq]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanothermococcus_thermolithotrophicus Methanothermococcus thermolithotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ESQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ESQ FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetyl-CoA_C-acetyltransferase Acetyl-CoA C-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.9 2.3.1.9] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6esq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6esq OCA], [http://pdbe.org/6esq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6esq RCSB], [http://www.ebi.ac.uk/pdbsum/6esq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6esq ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Acetyl-CoA C-acetyltransferase]]
		+	[[Category: Methanothermococcus thermolithotrophicus]]
		+	[[Category: Engilberge, S]]
		+	[[Category: Erb, J T]]
		+	[[Category: Girard, E]]
		+	[[Category: Maury, O]]
		+	[[Category: Riobe, F]]
		+	[[Category: Shima, S]]
		+	[[Category: Voegeli, B]]
		+	[[Category: Wagner, T]]
		+	[[Category: Archaea]]
		+	[[Category: Duf35 family]]
		+	[[Category: Enzymatic channeling]]
		+	[[Category: Isoprenoid synthesis]]
		+	[[Category: Lipid biosynthesis]]
		+	[[Category: Mevalonate production]]
		+	[[Category: Multi-enzymatic complex]]
		+	[[Category: Scaffold protein]]
		+	[[Category: Transferase]]

OCA: Protected "6esq" [edit=sysop:move=sysop]

2017-11-01T05:05:25Z

Protected "6esq" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:05, 1 November 2017

OCA: New page: '''Unreleased structure''' The entry 6esq is ON HOLD Authors: Description: Category: Unreleased Structures

2017-11-01T05:05:24Z

New page: '''Unreleased structure''' The entry 6esq is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6esq is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]