6et6 - Revision history

OCA at 09:15, 11 November 2020

2020-11-11T09:15:01Z

←Older revision		Revision as of 09:15, 11 November 2020
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	==Crystal structure of muramidase from Acinetobacter baumannii AB 5075UW prophage==		==Crystal structure of muramidase from Acinetobacter baumannii AB 5075UW prophage==
-	<StructureSection load='6et6' size='340' side='right' caption='[[6et6]], [[Resolution\|resolution]] 1.20Å' scene=''>	+	<StructureSection load='6et6' size='340' side='right'caption='[[6et6]], [[Resolution\|resolution]] 1.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6et6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ET6 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ET6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6et6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ET6 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ET6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6et6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6et6 OCA], [http://pdbe.org/6et6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6et6 RCSB], [http://www.ebi.ac.uk/pdbsum/6et6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6et6 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6et6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6et6 OCA], [http://pdbe.org/6et6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6et6 RCSB], [http://www.ebi.ac.uk/pdbsum/6et6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6et6 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 6et6" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6et6" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Lysozyme 3D structures\|Lysozyme 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
	[[Category: Boyko, K M]]		[[Category: Boyko, K M]]

OCA at 10:11, 5 September 2018

2018-09-05T10:11:03Z

←Older revision		Revision as of 10:11, 5 September 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6et6 is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of muramidase from Acinetobacter baumannii AB 5075UW prophage==
		+	<StructureSection load='6et6' size='340' side='right' caption='[[6et6]], [[Resolution\|resolution]] 1.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6et6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ET6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ET6 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6et6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6et6 OCA], [http://pdbe.org/6et6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6et6 RCSB], [http://www.ebi.ac.uk/pdbsum/6et6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6et6 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Proteins that include enzymatic domain degrading the bacterial cell wall and a domain providing transport through the bacterial outer membrane are considered as prospective compounds to combat pathogenic Gram-negative bacteria. This paper presents an isolation and study of an enzyme of this class naturally encoded in the prophage region of Acinetobacter baumannii AB 5075 genome. Recombinant protein expressed in E. coli exhibits an antimicrobial activity with respect to live cultures of Gram-negative bacteria reducing the population of viable bacteria by 1.5(-)2 log colony forming units (CFU)/mL. However the protein becomes rapidly inactivated and enables the bacteria to restore the population. AcLys structure determined by X-ray crystallography reveals a predominantly α—helical fold similar to bacteriophage P22 lysozyme. The capital ES, Cyrillic-terminal part of AcLys polypeptide chains forms an α—helix enriched by Lys and Arg residues exposed outside of the protein globule. Presumably this type of structure of the C-terminal α—helix has evolved evolutionally enabling the endolysin to pass the inner membrane during the host lysis or, potentially, to penetrate the outer membrane of the Gram-negative bacteria.

-	~~Authors: Boyko~~, ~~K.M.~~, Nikolaeva, ~~A.Y.~~, ~~Sykilinda~~, ~~N.N.~~, ~~Shneider~~, ~~M.M.~~, Miroshnikov~~, K~~.A.~~, Popov, V~~.O.	+	Structure of an Acinetobacter Broad-Range Prophage Endolysin Reveals a C-Terminal alpha-Helix with the Proposed Role in Activity against Live Bacterial Cells.,Sykilinda NN, Nikolaeva AY, Shneider MM, Mishkin DV, Patutin AA, Popov VO, Boyko KM, Klyachko NL, Miroshnikov KA Viruses. 2018 Jun 6;10(6). pii: v10060309. doi: 10.3390/v10060309. PMID:29882827<ref>PMID:29882827</ref>

-	~~Description: Crystal structure~~ of ~~muramidase from Acinetobacter baumannii AB 5075UW prophage~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Popov~~, ~~V.O~~]]	+	<div class="pdbe-citations 6et6" style="background-color:#fffaf0;"></div>
-	[[Category: Nikolaeva, A.Y]]	+	== References ==
-	[[Category: ~~Miroshnikov~~, ~~K.A~~]]	+	<references/>
-	[[Category: Shneider, M.M]]	+	__TOC__
-	[[Category: Sykilinda, N.N]]	+	</StructureSection>
-	[[Category: ~~Boyko, K.M~~]]	+	[[Category: Lysozyme]]
		+	[[Category: Boyko, K M]]
		+	[[Category: Miroshnikov, K A]]
		+	[[Category: Nikolaeva, A Y]]
		+	[[Category: Popov, V O]]
		+	[[Category: Shneider, M M]]
		+	[[Category: Sykilinda, N N]]
		+	[[Category: Acinetobacter baumannii]]
		+	[[Category: Antimicrobial protein]]
		+	[[Category: Muramidase]]

OCA at 06:40, 8 November 2017

2017-11-08T06:40:42Z

←Older revision		Revision as of 06:40, 8 November 2017
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6et6 is ON HOLD	+	The entry 6et6 is ON HOLD until Paper Publication

-	Authors: Boyko, K.M., Nikolaeva, A.Y., Sykilinda, N.N., ~~Mishkin~~, D.V., Miroshnikov, K.A., Popov, V.O.	+	Authors: Boyko, K.M., Nikolaeva, A.Y., Sykilinda, N.N., Shneider, M.M., Miroshnikov, K.A., Popov, V.O.

-	Description: Crystal structure of muramidase from Acinetobacter baumannii AB 5075UW	+	Description: Crystal structure of muramidase from Acinetobacter baumannii AB 5075UW prophage
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Mishkin, D.V]]
	[[Category: Popov, V.O]]		[[Category: Popov, V.O]]
	[[Category: Nikolaeva, A.Y]]		[[Category: Nikolaeva, A.Y]]
	[[Category: Miroshnikov, K.A]]		[[Category: Miroshnikov, K.A]]
		+	[[Category: Shneider, M.M]]
	[[Category: Sykilinda, N.N]]		[[Category: Sykilinda, N.N]]
	[[Category: Boyko, K.M]]		[[Category: Boyko, K.M]]

OCA: Protected "6et6" [edit=sysop:move=sysop]

2017-11-01T05:06:39Z

Protected "6et6" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:06, 1 November 2017

OCA: New page: '''Unreleased structure''' The entry 6et6 is ON HOLD Authors: Boyko, K.M., Nikolaeva, A.Y., Sykilinda, N.N., Mishkin, D.V., Miroshnikov, K.A., Popov, V.O. Description: Crystal structur...

2017-11-01T05:06:38Z

New page: '''Unreleased structure''' The entry 6et6 is ON HOLD Authors: Boyko, K.M., Nikolaeva, A.Y., Sykilinda, N.N., Mishkin, D.V., Miroshnikov, K.A., Popov, V.O. Description: Crystal structur...

New page

'''Unreleased structure'''

The entry 6et6 is ON HOLD

Authors: Boyko, K.M., Nikolaeva, A.Y., Sykilinda, N.N., Mishkin, D.V., Miroshnikov, K.A., Popov, V.O.

Description: Crystal structure of muramidase from Acinetobacter baumannii AB 5075UW
[[Category: Unreleased Structures]]
[[Category: Mishkin, D.V]]
[[Category: Popov, V.O]]
[[Category: Nikolaeva, A.Y]]
[[Category: Miroshnikov, K.A]]
[[Category: Sykilinda, N.N]]
[[Category: Boyko, K.M]]