6euj - Revision history

OCA at 07:11, 16 October 2019

2019-10-16T07:11:18Z

←Older revision		Revision as of 07:11, 16 October 2019
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	</div>		</div>
	<div class="pdbe-citations 6euj" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6euj" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glucanase 3D structures\|Glucanase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:10, 1 May 2019

2019-05-01T09:10:58Z

←Older revision		Revision as of 09:10, 1 May 2019
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	==The GH43, Beta 1,3 Galactosidase, BT0265==		==The GH43, Beta 1,3 Galactosidase, BT0265==
-	<StructureSection load='6euj' size='340' side='right' caption='[[6euj]], [[Resolution\|resolution]] 2.75Å' scene=''>	+	<StructureSection load='6euj' size='340' side='right'caption='[[6euj]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6euj]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EUJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EUJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6euj]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bactn Bactn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EUJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EUJ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6euj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6euj OCA], [http://pdbe.org/6euj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6euj RCSB], [http://www.ebi.ac.uk/pdbsum/6euj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6euj ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BT_0265 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=226186 BACTN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6euj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6euj OCA], [http://pdbe.org/6euj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6euj RCSB], [http://www.ebi.ac.uk/pdbsum/6euj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6euj ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Glycans are major nutrients for the human gut microbiota (HGM). Arabinogalactan proteins (AGPs) comprise a heterogenous group of plant glycans in which a beta1,3-galactan backbone and beta1,6-galactan side chains are conserved. Diversity is provided by the variable nature of the sugars that decorate the galactans. The mechanisms by which nutritionally relevant AGPs are degraded in the HGM are poorly understood. Here we explore how the HGM organism Bacteroides thetaiotaomicron metabolizes AGPs. We propose a sequential degradative model in which exo-acting glycoside hydrolase (GH) family 43 beta1,3-galactanases release the side chains. These oligosaccharide side chains are depolymerized by the synergistic action of exo-acting enzymes in which catalytic interactions are dependent on whether degradation is initiated by a lyase or GH. We identified two GHs that establish two previously undiscovered GH families. The crystal structures of the exo-beta1,3-galactanases identified a key specificity determinant and departure from the canonical catalytic apparatus of GH43 enzymes. Growth studies of Bacteroidetes spp. on complex AGP revealed 3 keystone organisms that facilitated utilization of the glycan by 17 recipient bacteria, which included B. thetaiotaomicron. A surface endo-beta1,3-galactanase, when engineered into B. thetaiotaomicron, enabled the bacterium to utilize complex AGPs and act as a keystone organism.
		+
		+	A surface endogalactanase in Bacteroides thetaiotaomicron confers keystone status for arabinogalactan degradation.,Cartmell A, Munoz-Munoz J, Briggs JA, Ndeh DA, Lowe EC, Basle A, Terrapon N, Stott K, Heunis T, Gray J, Yu L, Dupree P, Fernandes PZ, Shah S, Williams SJ, Labourel A, Trost M, Henrissat B, Gilbert HJ Nat Microbiol. 2018 Nov;3(11):1314-1326. doi: 10.1038/s41564-018-0258-8. Epub, 2018 Oct 22. PMID:30349080<ref>PMID:30349080</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6euj" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bactn]]
		+	[[Category: Large Structures]]
	[[Category: Cartmell, A]]		[[Category: Cartmell, A]]
	[[Category: Gilbert, H J]]		[[Category: Gilbert, H J]]

OCA at 08:04, 17 October 2018

2018-10-17T08:04:18Z

←Older revision		Revision as of 08:04, 17 October 2018
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-	'''Unreleased structure'''

-	The ~~entry~~ 6euj ~~is ON HOLD~~	+	==The GH43, Beta 1,3 Galactosidase, BT0265==
-		+	<StructureSection load='6euj' size='340' side='right' caption='[[6euj]], [[Resolution\|resolution]] 2.75Å' scene=''>
-	~~Authors~~: ~~Cartmell, A~~.~~, Gilbert, H~~.J.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6euj]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EUJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EUJ FirstGlance]. <br>
-	~~Description~~: ~~The GH43~~, ~~Beta 1~~,~~3 Glacatosidase~~, ~~BT0265~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6euj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6euj OCA], [http://pdbe.org/6euj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6euj RCSB], [http://www.ebi.ac.uk/pdbsum/6euj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6euj ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
	[[Category: Cartmell, A]]		[[Category: Cartmell, A]]
-	[[Category: Gilbert, H.J]]	+	[[Category: Gilbert, H J]]
		+	[[Category: Galactosidase]]
		+	[[Category: Arabiogalactan]]
		+	[[Category: Beta 1]]
		+	[[Category: Gh43]]
		+	[[Category: Hydrolase]]

OCA: Protected "6euj" [edit=sysop:move=sysop]

2017-11-08T06:42:54Z

Protected "6euj" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:42, 8 November 2017

OCA: New page: '''Unreleased structure''' The entry 6euj is ON HOLD Authors: Cartmell, A., Gilbert, H.J. Description: The GH43, Beta 1,3 Glacatosidase, BT0265 Category: Unreleased Structures [[Ca...

2017-11-08T06:42:53Z

New page: '''Unreleased structure''' The entry 6euj is ON HOLD Authors: Cartmell, A., Gilbert, H.J. Description: The GH43, Beta 1,3 Glacatosidase, BT0265 Category: Unreleased Structures [[Ca...

New page

'''Unreleased structure'''

The entry 6euj is ON HOLD

Authors: Cartmell, A., Gilbert, H.J.

Description: The GH43, Beta 1,3 Glacatosidase, BT0265
[[Category: Unreleased Structures]]
[[Category: Cartmell, A]]
[[Category: Gilbert, H.J]]