6eus - Revision history

OCA at 06:25, 19 June 2019

2019-06-19T06:25:48Z

←Older revision		Revision as of 06:25, 19 June 2019
Line 1:		Line 1:

	==Crystal structure of the outer membrane channel DcaP of Acinetobacter baumannii==		==Crystal structure of the outer membrane channel DcaP of Acinetobacter baumannii==
-	<StructureSection load='6eus' size='340' side='right' caption='[[6eus]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='6eus' size='340' side='right'caption='[[6eus]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6eus]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EUS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EUS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6eus]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Aciba Aciba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EUS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EUS FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ABUW_0826, B4R90_15245, CAS84_19480, CAT05_14765, CBI29_03066, IX87_10345 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=470 ACIBA])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6eus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eus OCA], [http://pdbe.org/6eus PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eus RCSB], [http://www.ebi.ac.uk/pdbsum/6eus PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eus ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6eus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eus OCA], [http://pdbe.org/6eus PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eus RCSB], [http://www.ebi.ac.uk/pdbsum/6eus PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eus ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Research efforts to discover potential new antibiotics for Gram-negative bacteria suffer from high attrition rates due to the synergistic action of efflux systems and the limited permeability of the outer membrane (OM). One strategy to overcome the OM permeability barrier is to identify small molecules that are natural substrates for abundant OM channels and use such compounds as scaffolds for the design of efficiently permeating antibacterials. Here we present a multidisciplinary approach to identify such potential small-molecule scaffolds. Focusing on the pathogenic bacterium Acinetobacter baumannii, we use OM proteomics to identify DcaP as the most abundant channel during infection in rodents. The X-ray crystal structure of DcaP reveals a trimeric, porin-like structure and suggests that dicarboxylic acids are potential transport substrates. Electrophysiological experiments and all-atom molecular dynamics simulations confirm this notion and provide atomistic information on likely permeation pathways and energy barriers for several small molecules, including a clinically relevant beta-lactamase inhibitor.
		+
		+	A Multidisciplinary Approach toward Identification of Antibiotic Scaffolds for Acinetobacter baumannii.,Bhamidimarri SP, Zahn M, Prajapati JD, Schleberger C, Soderholm S, Hoover J, West J, Kleinekathofer U, Bumann D, Winterhalter M, van den Berg B Structure. 2019 Feb 5;27(2):268-280.e6. doi: 10.1016/j.str.2018.10.021. Epub 2018, Dec 13. PMID:30554842<ref>PMID:30554842</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6eus" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aciba]]
		+	[[Category: Large Structures]]
	[[Category: Berg, B van den]]		[[Category: Berg, B van den]]
	[[Category: Zahn, M]]		[[Category: Zahn, M]]

OCA at 08:18, 14 November 2018

2018-11-14T08:18:00Z

←Older revision		Revision as of 08:18, 14 November 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6eus is ON HOLD~~	+	==Crystal structure of the outer membrane channel DcaP of Acinetobacter baumannii==
-		+	<StructureSection load='6eus' size='340' side='right' caption='[[6eus]], [[Resolution\|resolution]] 2.20Å' scene=''>
-	~~Authors: Zahn, M., van den Berg, B.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6eus]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EUS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EUS FirstGlance]. <br>
-	~~Description:~~ Crystal structure of the outer membrane channel DcaP of Acinetobacter baumannii	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6eus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eus OCA], [http://pdbe.org/6eus PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eus RCSB], [http://www.ebi.ac.uk/pdbsum/6eus PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eus ProSAT]</span></td></tr>
-	[[Category: ~~Van Den~~ Berg, B]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Berg, B van den]]
	[[Category: Zahn, M]]		[[Category: Zahn, M]]
		+	[[Category: Acinetobacter baumannii]]
		+	[[Category: Membrane protein]]
		+	[[Category: Outer membrane protein]]

OCA: Protected "6eus" [edit=sysop:move=sysop]

2017-11-08T06:43:26Z

Protected "6eus" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:43, 8 November 2017

OCA: New page: '''Unreleased structure''' The entry 6eus is ON HOLD Authors: Zahn, M., van den Berg, B. Description: Crystal structure of the outer membrane channel DcaP of Acinetobacter baumannii [[...

2017-11-08T06:43:24Z

New page: '''Unreleased structure''' The entry 6eus is ON HOLD Authors: Zahn, M., van den Berg, B. Description: Crystal structure of the outer membrane channel DcaP of Acinetobacter baumannii [[...

New page

'''Unreleased structure'''

The entry 6eus is ON HOLD

Authors: Zahn, M., van den Berg, B.

Description: Crystal structure of the outer membrane channel DcaP of Acinetobacter baumannii
[[Category: Unreleased Structures]]
[[Category: Van Den Berg, B]]
[[Category: Zahn, M]]