6evk - Revision history

OCA at 06:49, 7 February 2018

2018-02-07T06:49:10Z

←Older revision		Revision as of 06:49, 7 February 2018
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	<StructureSection load='6evk' size='340' side='right' caption='[[6evk]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='6evk' size='340' side='right' caption='[[6evk]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6evk]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EVK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EVK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6evk]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus_(a/little_yellow-shouldered_bat/guatemala/060/2010(h17n10)) Influenza a virus (a/little yellow-shouldered bat/guatemala/060/2010(h17n10))]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EVK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EVK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MGP:7-METHYL-GUANOSINE-5-TRIPHOSPHATE'>MGP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MGP:7-METHYL-GUANOSINE-5-TRIPHOSPHATE'>MGP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1129347 Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))]), PB1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1129347 Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))]), PB2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1129347 Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6evk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6evk OCA], [http://pdbe.org/6evk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6evk RCSB], [http://www.ebi.ac.uk/pdbsum/6evk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6evk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6evk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6evk OCA], [http://pdbe.org/6evk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6evk RCSB], [http://www.ebi.ac.uk/pdbsum/6evk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6evk ProSAT]</span></td></tr>

OCA at 07:06, 13 December 2017

2017-12-13T07:06:32Z

←Older revision		Revision as of 07:06, 13 December 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 6evk is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of bat influenza A/H17N10 polymerase with viral RNA promoter and cap analogue m7GTP==
		+	<StructureSection load='6evk' size='340' side='right' caption='[[6evk]], [[Resolution\|resolution]] 2.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6evk]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EVK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EVK FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MGP:7-METHYL-GUANOSINE-5-TRIPHOSPHATE'>MGP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6evk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6evk OCA], [http://pdbe.org/6evk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6evk RCSB], [http://www.ebi.ac.uk/pdbsum/6evk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6evk ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Influenza polymerase uses short capped primers snatched from nascent Pol II transcripts to initiate transcription of viral mRNAs. Here we describe crystal structures of influenza A and B polymerase bound to a capped primer in a configuration consistent with transcription initiation ('priming state') and show by functional assays that conserved residues from both the PB2 midlink and cap-binding domains are important for positioning the capped RNA. In particular, mutation of PB2 Arg264, which interacts with the triphosphate linkage in the cap, significantly and specifically decreases cap-dependent transcription. We also compare the configuration of the midlink and cap-binding domains in the priming state with their very different relative arrangement (called the 'apo' state) in structures where the potent cap-binding inhibitor VX-787, or a close analogue, is bound. In the 'apo' state the inhibitor makes additional interactions to the midlink domain that increases its affinity beyond that to the cap-binding domain alone. The comparison suggests that the mechanism of resistance of certain mutations that allow virus to escape from VX-787, notably PB2 N510T, can only be rationalized if VX-787 has a dual mode of action, direct inhibition of capped RNA binding as well as stabilization of the transcriptionally inactive 'apo' state.

-	~~Authors: Pflug~~, A., ~~Cusack~~, S.	+	Capped RNA primer binding to influenza polymerase and implications for the mechanism of cap-binding inhibitors.,Pflug A, Gaudon S, Resa-Infante P, Lethier M, Reich S, Schulze WM, Cusack S Nucleic Acids Res. 2017 Nov 30. pii: 4675317. doi: 10.1093/nar/gkx1210. PMID:29202182<ref>PMID:29202182</ref>

-	~~Description: Crystal structure~~ of ~~bat influenza A~~/~~H17N10 polymerase with viral RNA promoter and cap analogue m7GTP~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Pflug, A~~]]	+	<div class="pdbe-citations 6evk" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: RNA-directed RNA polymerase]]
	[[Category: Cusack, S]]		[[Category: Cusack, S]]
		+	[[Category: Pflug, A]]
		+	[[Category: Cap analogue]]
		+	[[Category: Influenza virus]]
		+	[[Category: Rna-dependent rna polymerase]]
		+	[[Category: Viral protein]]

OCA at 06:28, 15 November 2017

2017-11-15T06:28:09Z

←Older revision		Revision as of 06:28, 15 November 2017
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6evk is ON HOLD	+	The entry 6evk is ON HOLD until Paper Publication

	Authors: Pflug, A., Cusack, S.		Authors: Pflug, A., Cusack, S.

OCA: Protected "6evk" [edit=sysop:move=sysop]

2017-11-08T06:44:59Z

Protected "6evk" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:44, 8 November 2017

OCA: New page: '''Unreleased structure''' The entry 6evk is ON HOLD Authors: Pflug, A., Cusack, S. Description: Crystal structure of bat influenza A/H17N10 polymerase with viral RNA promoter and cap ...

2017-11-08T06:44:58Z

New page: '''Unreleased structure''' The entry 6evk is ON HOLD Authors: Pflug, A., Cusack, S. Description: Crystal structure of bat influenza A/H17N10 polymerase with viral RNA promoter and cap ...

New page

'''Unreleased structure'''

The entry 6evk is ON HOLD

Authors: Pflug, A., Cusack, S.

Description: Crystal structure of bat influenza A/H17N10 polymerase with viral RNA promoter and cap analogue m7GTP
[[Category: Unreleased Structures]]
[[Category: Pflug, A]]
[[Category: Cusack, S]]