6fbr - Revision history

OCA at 06:47, 9 January 2019

2019-01-09T06:47:01Z

←Older revision		Revision as of 06:47, 9 January 2019
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	<StructureSection load='6fbr' size='340' side='right' caption='[[6fbr]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='6fbr' size='340' side='right' caption='[[6fbr]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6fbr]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FBR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FBR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6fbr]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FBR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FBR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RXRA, NR2B1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fbr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fbr OCA], [http://pdbe.org/6fbr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fbr RCSB], [http://www.ebi.ac.uk/pdbsum/6fbr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fbr ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fbr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fbr OCA], [http://pdbe.org/6fbr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fbr RCSB], [http://www.ebi.ac.uk/pdbsum/6fbr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fbr ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: McEwen, A G]]		[[Category: McEwen, A G]]
	[[Category: Osz, J]]		[[Category: Osz, J]]

OCA at 06:34, 5 December 2018

2018-12-05T06:34:55Z

←Older revision		Revision as of 06:34, 5 December 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6fbr is ~~ON HOLD~~	+	==Crystal Structure of the Human Retinoid X Receptor DNA-Binding Domain Bound to the Human MEp DR1 Response Element, pH 4.2==
		+	<StructureSection load='6fbr' size='340' side='right' caption='[[6fbr]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6fbr]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FBR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FBR FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fbr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fbr OCA], [http://pdbe.org/6fbr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fbr RCSB], [http://www.ebi.ac.uk/pdbsum/6fbr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fbr ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RXRA_HUMAN RXRA_HUMAN]] Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes.<ref>PMID:10195690</ref> <ref>PMID:11162439</ref> <ref>PMID:11915042</ref> <ref>PMID:20215566</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Retinoid X Receptors (RXRs) act as dimer partners for several nuclear receptors including itself, binding to genomic DNA response elements and regulating gene transcription with cell and gene specificity. As homodimers, RXRs bind direct repeats of the half-site (A/G)G(G/T)TCA separated by 1 nucleotide (DR1) and little variability of this consensus site is observed for natural DR1s. However, these variations are responsible of the modulation of RXR receptors function through differential binding affinity and conformational changes. To further our understanding of the molecular mechanisms underlying RXR-DNA interactions, we examined how RXR DBDs bind to different DR1s using thermodynamics, X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy. We show that the half-site sequences modulate the binding cooperativity that results from the protein-protein contacts between the two DBDs. Chemical shifts perturbation NMR experiments revealed that sequence variations in half-sites induce changes that propagate from the protein-DNA interface to the dimerization interface throughout the DBD fold.

-	~~Authors:~~ McEwen, ~~A.G.~~, Poussin-Courmontagne, P., ~~Osz~~, J., ~~Rochel~~, N.	+	Modulation of RXR-DNA complex assembly by DNA context.,Osz J, McEwen AG, Wolf J, Poussin-Courmontagne P, Peluso-Iltis C, Chebaro Y, Kieffer B, Rochel N Mol Cell Endocrinol. 2018 Nov 23. pii: S0303-7207(18)30340-X. doi:, 10.1016/j.mce.2018.11.008. PMID:30476562<ref>PMID:30476562</ref>

-	~~Description: Crystal Structure~~ of the ~~Human Retinoid X Receptor DNA-Binding Domain Bound to the Human MEp DR1 Response Element, pH 4~~.2	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Poussin-Courmontagne~~, P]]	+	<div class="pdbe-citations 6fbr" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: McEwen, A G]]
	[[Category: Osz, J]]		[[Category: Osz, J]]
-	[[Category: ~~Mcewen~~, ~~A.G~~]]	+	[[Category: Poussin-Courmontagne, P]]
	[[Category: Rochel, N]]		[[Category: Rochel, N]]
		+	[[Category: Nuclear receptor]]
		+	[[Category: Transcription]]
		+	[[Category: Transcription-dna complex]]

OCA: Protected "6fbr" [edit=sysop:move=sysop]

2017-12-27T07:32:18Z

Protected "6fbr" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:32, 27 December 2017

OCA: New page: '''Unreleased structure''' The entry 6fbr is ON HOLD Authors: McEwen, A.G., Poussin-Courmontagne, P., Osz, J., Rochel, N. Description: Crystal Structure of the Human Retinoid X Recepto...

2017-12-27T07:32:17Z

New page: '''Unreleased structure''' The entry 6fbr is ON HOLD Authors: McEwen, A.G., Poussin-Courmontagne, P., Osz, J., Rochel, N. Description: Crystal Structure of the Human Retinoid X Recepto...

New page

'''Unreleased structure'''

The entry 6fbr is ON HOLD

Authors: McEwen, A.G., Poussin-Courmontagne, P., Osz, J., Rochel, N.

Description: Crystal Structure of the Human Retinoid X Receptor DNA-Binding Domain Bound to the Human MEp DR1 Response Element, pH 4.2
[[Category: Unreleased Structures]]
[[Category: Poussin-Courmontagne, P]]
[[Category: Osz, J]]
[[Category: Mcewen, A.G]]
[[Category: Rochel, N]]