6fdj - Revision history

OCA at 12:30, 17 July 2019

2019-07-17T12:30:40Z

←Older revision		Revision as of 12:30, 17 July 2019
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	==Crystal Structure of Two-Domain Laccase mutant H165A from Streptomyces griseoflavus with high copper ions occupancy==		==Crystal Structure of Two-Domain Laccase mutant H165A from Streptomyces griseoflavus with high copper ions occupancy==
-	<StructureSection load='6fdj' size='340' side='right' caption='[[6fdj]], [[Resolution\|resolution]] 2.31Å' scene=''>	+	<StructureSection load='6fdj' size='340' side='right'caption='[[6fdj]], [[Resolution\|resolution]] 2.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6fdj]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FDJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FDJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6fdj]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_griseoflavus"_krainsky_1914 "actinomyces griseoflavus" krainsky 1914]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FDJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FDJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6fc7\|6fc7]], [[5mkm\|5mkm]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6fc7\|6fc7]], [[5mkm\|5mkm]]</td></tr>
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fdj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fdj OCA], [http://pdbe.org/6fdj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fdj RCSB], [http://www.ebi.ac.uk/pdbsum/6fdj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fdj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fdj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fdj OCA], [http://pdbe.org/6fdj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fdj RCSB], [http://www.ebi.ac.uk/pdbsum/6fdj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fdj ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Laccases (EC 1.10.3.2) are multicopper oxidoreductases acting on diphenols and related substances. Laccases are highly important for biotechnology and environmental remediation. These enzymes contain mononuclear one T2 copper ion and two T3 copper ions (Cu3alpha and Cu3beta), which form the so-called trinuclear center (TNC). Along with the typical three-domain laccases Bacteria produce two-domain (2D) enzymes, which are active at neutral and basic pH, thermostable, and resistant to inhibitors. In this work we present the comparative analysis of crystal structures and catalytic properties of recombinant 2D laccase from Streptomyces griseoflavus Ac-993 (SgfSL) and its four mutant forms with replacements of two amino acid residues, located at the narrowing of the presumable T3-solvent tunnels. We obtained inactive enzymes with substitutions of His165, with Phe, and Ile170 with Ala or Phe. His165Ala variant was more active than the wild type. We suggest that His165 is a "gateway" at the O2-tunnel leading from solvent to the Cu3beta of the enzyme. The side chain of Ile170 could be indirectly involved in the coordination of copper ions at the T3 center by maintaining the position of the imidazole ring of His157 that belongs to the first coordination sphere of Cu3alpha.
		+
		+	Investigations of Accessibility of T2/T3 Copper Center of Two-Domain Laccase from Streptomyces griseoflavus Ac-993.,Gabdulkhakov A, Kolyadenko I, Kostareva O, Mikhaylina A, Oliveira P, Tamagnini P, Lisov A, Tishchenko S Int J Mol Sci. 2019 Jun 28;20(13). pii: ijms20133184. doi: 10.3390/ijms20133184. PMID:31261802<ref>PMID:31261802</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6fdj" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Laccase 3D structures\|Laccase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Actinomyces griseoflavus krainsky 1914]]
	[[Category: Laccase]]		[[Category: Laccase]]
		+	[[Category: Large Structures]]
	[[Category: Gabdulkhakov, A G]]		[[Category: Gabdulkhakov, A G]]
	[[Category: Tishchenko, T V]]		[[Category: Tishchenko, T V]]

OCA at 08:20, 30 January 2019

2019-01-30T08:20:20Z

←Older revision		Revision as of 08:20, 30 January 2019
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-	'''Unreleased structure'''

-	~~The entry 6fdj is ON HOLD~~	+	==Crystal Structure of Two-Domain Laccase mutant H165A from Streptomyces griseoflavus with high copper ions occupancy==
-		+	<StructureSection load='6fdj' size='340' side='right' caption='[[6fdj]], [[Resolution\|resolution]] 2.31Å' scene=''>
-	~~Authors: Gabdulkhakov, A.G., Tishchenko, T.V.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6fdj]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FDJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FDJ FirstGlance]. <br>
-	~~Description:~~ Crystal Structure of Two-Domain Laccase mutant H165A from Streptomyces griseoflavus with high copper ions occupancy	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6fc7\|6fc7]], [[5mkm\|5mkm]]</td></tr>
-	[[~~Category~~: ~~Tishchenko~~, T.V]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>
-	[[Category: Gabdulkhakov, A.G]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fdj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fdj OCA], [http://pdbe.org/6fdj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fdj RCSB], [http://www.ebi.ac.uk/pdbsum/6fdj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fdj ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Laccase]]
		+	[[Category: Gabdulkhakov, A G]]
		+	[[Category: Tishchenko, T V]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Streptomyces griseoflavus]]
		+	[[Category: Two-domain laccase]]

OCA: Protected "6fdj" [edit=sysop:move=sysop]

2018-01-03T04:57:51Z

Protected "6fdj" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:57, 3 January 2018

OCA: New page: '''Unreleased structure''' The entry 6fdj is ON HOLD Authors: Gabdulkhakov, A.G., Tishchenko, T.V. Description: Crystal Structure of Two-Domain Laccase mutant H165A from Streptomyces g...

2018-01-03T04:57:50Z

New page: '''Unreleased structure''' The entry 6fdj is ON HOLD Authors: Gabdulkhakov, A.G., Tishchenko, T.V. Description: Crystal Structure of Two-Domain Laccase mutant H165A from Streptomyces g...

New page

'''Unreleased structure'''

The entry 6fdj is ON HOLD

Authors: Gabdulkhakov, A.G., Tishchenko, T.V.

Description: Crystal Structure of Two-Domain Laccase mutant H165A from Streptomyces griseoflavus with high copper ions occupancy
[[Category: Unreleased Structures]]
[[Category: Tishchenko, T.V]]
[[Category: Gabdulkhakov, A.G]]