6fvr - Revision history

OCA at 12:31, 9 May 2024

2024-05-09T12:31:17Z

←Older revision		Revision as of 12:31, 9 May 2024
Line 3:		Line 3:
	<StructureSection load='6fvr' size='340' side='right'caption='[[6fvr]], [[Resolution\|resolution]] 4.20Å' scene=''>		<StructureSection load='6fvr' size='340' side='right'caption='[[6fvr]], [[Resolution\|resolution]] 4.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6fvr]] is a 5 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Endosymbiont_of_tevnia_jerichonana_~~(~~vent_tica~~) Endosymbiont of ~~tevnia~~ jerichonana (vent ~~tica~~)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FVR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6FVR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6fvr]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Endosymbiont_of_Tevnia_jerichonana_(vent_Tica) Endosymbiont of Tevnia jerichonana (vent Tica)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FVR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6FVR FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=BNG:B~~-~~NONYLGLUCOSIDE'>BNG</scene>~~, ~~<scene name='pdbligand=CS:CESIUM+ION'>CS</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 4.2Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~TevJSym_bc00020 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=1049564 endosymbiont of Tevnia jerichonana (vent Tica)])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=CS:CESIUM+ION'>CS</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6fvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fvr OCA], [~~http~~://pdbe.org/6fvr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6fvr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6fvr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6fvr ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6fvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fvr OCA], [https://pdbe.org/6fvr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6fvr RCSB], [https://www.ebi.ac.uk/pdbsum/6fvr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6fvr ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G2FID1_9GAMM G2FID1_9GAMM]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 6fvr" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6fvr" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ion channels 3D structures\|Ion channels 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 22:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Delarue, M]]	+	[[Category: Delarue M]]
-	[[Category: Hu, H]]	+	[[Category: Hu H]]
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Pentameric ligand-gated ion channel~~]]	+

OCA at 06:11, 12 June 2019

2019-06-12T06:11:06Z

←Older revision		Revision as of 06:11, 12 June 2019
Line 1:		Line 1:

	==The active form of a pentameric ion channel (sTeLIC) gated by alkaline pH - sTeLIC in complex with Cesium ions (Cs+)==		==The active form of a pentameric ion channel (sTeLIC) gated by alkaline pH - sTeLIC in complex with Cesium ions (Cs+)==
-	<StructureSection load='6fvr' size='340' side='right' caption='[[6fvr]], [[Resolution\|resolution]] 4.20Å' scene=''>	+	<StructureSection load='6fvr' size='340' side='right'caption='[[6fvr]], [[Resolution\|resolution]] 4.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6fvr]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FVR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FVR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6fvr]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Endosymbiont_of_tevnia_jerichonana_(vent_tica) Endosymbiont of tevnia jerichonana (vent tica)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FVR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FVR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=CS:CESIUM+ION'>CS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=CS:CESIUM+ION'>CS</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TevJSym_bc00020 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1049564 endosymbiont of Tevnia jerichonana (vent Tica)])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fvr OCA], [http://pdbe.org/6fvr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fvr RCSB], [http://www.ebi.ac.uk/pdbsum/6fvr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fvr ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fvr OCA], [http://pdbe.org/6fvr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fvr RCSB], [http://www.ebi.ac.uk/pdbsum/6fvr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fvr ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Delarue, M]]		[[Category: Delarue, M]]
-	[[Category: HU, H]]	+	[[Category: Hu, H]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]
	[[Category: Pentameric ligand-gated ion channel]]		[[Category: Pentameric ligand-gated ion channel]]

OCA at 05:38, 16 May 2018

2018-05-16T05:38:06Z

←Older revision		Revision as of 05:38, 16 May 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The ~~entry~~ 6fvr is ~~ON HOLD~~	+	==The active form of a pentameric ion channel (sTeLIC) gated by alkaline pH - sTeLIC in complex with Cesium ions (Cs+)==
		+	<StructureSection load='6fvr' size='340' side='right' caption='[[6fvr]], [[Resolution\|resolution]] 4.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6fvr]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FVR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FVR FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=CS:CESIUM+ION'>CS</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fvr OCA], [http://pdbe.org/6fvr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fvr RCSB], [http://www.ebi.ac.uk/pdbsum/6fvr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fvr ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Pentameric ligand-gated ion channels (pLGICs) constitute a widespread class of ion channels, present in archaea, bacteria, and eukaryotes. Upon binding of their agonists in the extracellular domain, the transmembrane pore opens, allowing ions to go through, via a gating mechanism that can be modulated by a number of drugs. Even though high-resolution structural information on pLGICs has increased in a spectacular way in recent years, both in bacterial and in eukaryotic systems, the structure of the open channel conformation of some intensively studied receptors whose structures are known in a nonactive (closed) form, such as Erwinia chrysanthemi pLGIC (ELIC), is still lacking. Here we describe a gammaproteobacterial pLGIC from an endo-symbiont of Tevnia jerichonana (sTeLIC), whose sequence is closely related to the pLGIC from ELIC with 28% identity. We provide an X-ray crystallographic structure at 2.3 A in an active conformation, where the pore is found to be more open than any current conformation found for pLGICs. In addition, two charged restriction rings are present in the vestibule. Functional characterization shows sTeLIC to be a cationic channel activated at alkaline pH. It is inhibited by divalent cations, but not by quaternary ammonium ions, such as tetramethylammonium. Additionally, we found that sTeLIC is allosterically potentiated by aromatic amino acids Phe and Trp, as well as their derivatives, such as 4-bromo-cinnamate, whose cocrystal structure reveals a vestibular binding site equivalent to, but more deeply buried than, the one already described for benzodiazepines in ELIC.

-	~~Authors: HU~~, H., ~~Delarue~~, M.	+	Crystal structures of a pentameric ion channel gated by alkaline pH show a widely open pore and identify a cavity for modulation.,Hu H, Nemecz A, Van Renterghem C, Fourati Z, Sauguet L, Corringer PJ, Delarue M Proc Natl Acad Sci U S A. 2018 Apr 24;115(17):E3959-E3968. doi:, 10.1073/pnas.1717700115. Epub 2018 Apr 9. PMID:29632192<ref>PMID:29632192</ref>

-	~~Description: The active form of~~ a ~~pentameric ion channel (sTeLIC) gated by alkaline pH -sTeLIC in complex with Cesium ions (Cs+)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Hu, H]]~~	+	<div class="pdbe-citations 6fvr" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Delarue, M]]		[[Category: Delarue, M]]
		+	[[Category: HU, H]]
		+	[[Category: Membrane protein]]
		+	[[Category: Pentameric ligand-gated ion channel]]

OCA: Protected "6fvr" [edit=sysop:move=sysop]

2018-03-14T05:15:28Z

Protected "6fvr" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:15, 14 March 2018

OCA: New page: '''Unreleased structure''' The entry 6fvr is ON HOLD Authors: HU, H., Delarue, M. Description: The active form of a pentameric ion channel (sTeLIC) gated by alkaline pH -sTeLIC in comp...

2018-03-14T05:15:26Z

New page: '''Unreleased structure''' The entry 6fvr is ON HOLD Authors: HU, H., Delarue, M. Description: The active form of a pentameric ion channel (sTeLIC) gated by alkaline pH -sTeLIC in comp...

New page

'''Unreleased structure'''

The entry 6fvr is ON HOLD

Authors: HU, H., Delarue, M.

Description: The active form of a pentameric ion channel (sTeLIC) gated by alkaline pH -sTeLIC in complex with Cesium ions (Cs+)
[[Category: Unreleased Structures]]
[[Category: Hu, H]]
[[Category: Delarue, M]]