OCA at 08:39, 17 October 2018

2018-10-17T08:39:50Z

←Older revision		Revision as of 08:39, 17 October 2018
Line 3:		Line 3:
	<StructureSection load='6g00' size='340' side='right' caption='[[6g00]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='6g00' size='340' side='right' caption='[[6g00]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6g00]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G00 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G00 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6g00]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Tertt Tertt]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G00 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G00 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TERTU_4506 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=377629 TERTT])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g00 OCA], [http://pdbe.org/6g00 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g00 RCSB], [http://www.ebi.ac.uk/pdbsum/6g00 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g00 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g00 OCA], [http://pdbe.org/6g00 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g00 RCSB], [http://www.ebi.ac.uk/pdbsum/6g00 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g00 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The biological conversion of lignocellulosic matter into high-value chemicals or biofuels is of increasing industrial importance as the sector slowly transitions away from nonrenewable sources. Many industrial processes involve the use of cellulolytic enzyme cocktails - a selection of glycoside hydrolases and, increasingly, polysaccharide oxygenases - to break down recalcitrant plant polysaccharides. ORFs from the genome of Teredinibacter turnerae, a symbiont hosted within the gills of marine shipworms, were identified in order to search for enzymes with desirable traits. Here, a putative T. turnerae glycoside hydrolase from family 8, hereafter referred to as TtGH8, is analysed. The enzyme is shown to be active against beta-1,4-xylan and mixed-linkage (beta-1,3,beta-1,4) marine xylan. Kinetic parameters, obtained using high-performance anion-exchange chromatography with pulsed amperometric detection and 3,5-dinitrosalicyclic acid reducing-sugar assays, show that TtGH8 catalyses the hydrolysis of beta-1,4-xylohexaose with a kcat/Km of 7.5 x 10(7) M(-1) min(-1) but displays maximal activity against mixed-linkage polymeric xylans, hinting at a primary role in the degradation of marine polysaccharides. The three-dimensional structure of TtGH8 was solved in uncomplexed and xylobiose-, xylotriose- and xylohexaose-bound forms at approximately 1.5 A resolution; the latter was consistent with the greater kcat/Km for hexasaccharide substrates. A (2,5)B boat conformation observed in the -1 position of bound xylotriose is consistent with the proposed conformational itinerary for this class of enzyme. This work shows TtGH8 to be effective at the degradation of xylan-based substrates, notably marine xylan, further exemplifying the potential of T. turnerae for effective and diverse biomass degradation.
		+
		+	Structure and function of a glycoside hydrolase family 8 endoxylanase from Teredinibacter turnerae.,Fowler CA, Hemsworth GR, Cuskin F, Hart S, Turkenburg J, Gilbert HJ, Walton PH, Davies GJ Acta Crystallogr D Struct Biol. 2018 Oct 1;74(Pt 10):946-955. doi:, 10.1107/S2059798318009737. Epub 2018 Oct 2. PMID:30289404<ref>PMID:30289404</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6g00" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Tertt]]
	[[Category: Davies, G J]]		[[Category: Davies, G J]]
	[[Category: Fowler, C A]]		[[Category: Fowler, C A]]

OCA at 08:12, 10 October 2018

2018-10-10T08:12:29Z

←Older revision		Revision as of 08:12, 10 October 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6g00 is ~~ON HOLD~~	+	==Crystal Structure of a GH8 xylanase from Teredinibacter turnerae==
-		+	<StructureSection load='6g00' size='340' side='right' caption='[[6g00]], [[Resolution\|resolution]] 1.40Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6g00]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G00 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G00 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g00 OCA], [http://pdbe.org/6g00 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g00 RCSB], [http://www.ebi.ac.uk/pdbsum/6g00 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g00 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Davies, G J]]
		+	[[Category: Fowler, C A]]
		+	[[Category: Walton, P H]]
		+	[[Category: Carbohydrate]]
		+	[[Category: Hydrolase]]
		+	[[Category: Native]]
		+	[[Category: Xylanse]]

OCA: Protected "6g00" [edit=sysop:move=sysop]

2018-03-21T06:25:15Z

Protected "6g00" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:25, 21 March 2018

OCA: New page: '''Unreleased structure''' The entry 6g00 is ON HOLD Authors: Description: Category: Unreleased Structures

2018-03-21T06:25:14Z

New page: '''Unreleased structure''' The entry 6g00 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6g00 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6g00 - Revision history

OCA at 08:39, 17 October 2018

OCA at 08:12, 10 October 2018

OCA: Protected "6g00" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6g00 is ON HOLD Authors: Description: Category: Unreleased Structures