6g74 - Revision history

OCA at 05:57, 30 May 2018

2018-05-30T05:57:55Z

←Older revision		Revision as of 05:57, 30 May 2018
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	<StructureSection load='6g74' size='340' side='right' caption='[[6g74]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6g74' size='340' side='right' caption='[[6g74]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6g74]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G74 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G74 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6g74]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thema Thema]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G74 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G74 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PHT:PHTHALIC+ACID'>PHT</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PHT:PHTHALIC+ACID'>PHT</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6f48\|6f48]], [[6f4d\|6f4d]], [[6f4l\|6f4l]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6f48\|6f48]], [[6f4d\|6f4d]], [[6f4l\|6f4l]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nadA, TM_1644 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 THEMA])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Quinolinate_synthase Quinolinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.72 2.5.1.72] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Quinolinate_synthase Quinolinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.72 2.5.1.72] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g74 OCA], [http://pdbe.org/6g74 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g74 RCSB], [http://www.ebi.ac.uk/pdbsum/6g74 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g74 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g74 OCA], [http://pdbe.org/6g74 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g74 RCSB], [http://www.ebi.ac.uk/pdbsum/6g74 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g74 ProSAT]</span></td></tr>
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	</StructureSection>		</StructureSection>
	[[Category: Quinolinate synthase]]		[[Category: Quinolinate synthase]]
		+	[[Category: Thema]]
	[[Category: Fontecilla-Camps, J C]]		[[Category: Fontecilla-Camps, J C]]
	[[Category: Volbeda, A]]		[[Category: Volbeda, A]]

OCA at 05:39, 25 April 2018

2018-04-25T05:39:47Z

←Older revision		Revision as of 05:39, 25 April 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6g74 is ~~ON HOLD~~	+	==Structure of the Y21F variant of quinolinate synthase in complex with phthalate==
		+	<StructureSection load='6g74' size='340' side='right' caption='[[6g74]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6g74]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G74 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G74 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PHT:PHTHALIC+ACID'>PHT</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6f48\|6f48]], [[6f4d\|6f4d]], [[6f4l\|6f4l]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Quinolinate_synthase Quinolinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.72 2.5.1.72] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g74 OCA], [http://pdbe.org/6g74 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g74 RCSB], [http://www.ebi.ac.uk/pdbsum/6g74 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g74 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NADA_THEMA NADA_THEMA]] Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	NadA is a multifunctional enzyme that condenses dihydroxyacetone phosphate (DHAP) with iminoaspartate (IA) to generate quinolinic acid (QA), the universal precursor of the nicotinamide adenine dinucleotide (NAD(P)) cofactor. Using X-ray crystallography, we have (i) characterized two of the reaction intermediates of QA synthesis using a "pH-shift" approach and a slowly reacting Thermotoga maritima NadA variant and (ii) observed the QA product, resulting from the degradation of an intermediate analogue, bound close to the entrance of a long tunnel leading to the solvent medium. We have also used molecular docking to propose a condensation mechanism between DHAP and IA based on two previously published Pyrococcus horikoshi NadA structures. The combination of reported data and our new results provide a structure-based complete catalytic sequence of QA synthesis by NadA.

-	~~Authors: Volbeda~~, A., Fontecilla-Camps~~, J~~.C.	+	Crystallographic Trapping of Reaction Intermediates in Quinolinic Acid Synthesis by NadA.,Volbeda A, Saez Cabodevilla J, Darnault C, Gigarel O, Han TH, Renoux O, Hamelin O, Ollagnier-de-Choudens S, Amara P, Fontecilla-Camps JC ACS Chem Biol. 2018 Apr 19. doi: 10.1021/acschembio.7b01104. PMID:29641168<ref>PMID:29641168</ref>

-	~~Description: Structure~~ of the ~~Y21F variant~~ of ~~quinolinate~~ synthase ~~in complex with phthalate~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6g74" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Quinolinate synthase]]
		+	[[Category: Fontecilla-Camps, J C]]
	[[Category: Volbeda, A]]		[[Category: Volbeda, A]]
-	[[Category: ~~Fontecilla-Camps, J.C~~]]	+	[[Category: Iron sulfur cluster]]
		+	[[Category: Nad biosynthesis]]
		+	[[Category: Transferase]]

OCA: Protected "6g74" [edit=sysop:move=sysop]

2018-04-11T12:41:46Z

Protected "6g74" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:41, 11 April 2018

OCA: New page: '''Unreleased structure''' The entry 6g74 is ON HOLD Authors: Volbeda, A., Fontecilla-Camps, J.C. Description: Structure of the Y21F variant of quinolinate synthase in complex with pht...

2018-04-11T12:41:45Z

New page: '''Unreleased structure''' The entry 6g74 is ON HOLD Authors: Volbeda, A., Fontecilla-Camps, J.C. Description: Structure of the Y21F variant of quinolinate synthase in complex with pht...

New page

'''Unreleased structure'''

The entry 6g74 is ON HOLD

Authors: Volbeda, A., Fontecilla-Camps, J.C.

Description: Structure of the Y21F variant of quinolinate synthase in complex with phthalate
[[Category: Unreleased Structures]]
[[Category: Volbeda, A]]
[[Category: Fontecilla-Camps, J.C]]