OCA at 12:33, 16 January 2019

2019-01-16T12:33:26Z

←Older revision		Revision as of 12:33, 16 January 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6geh is ~~ON HOLD~~	+	==Structure and reactivity of a siderophore-interacting protein from the marine bacterium Shewanella reveals unanticipated functional versatility.==
		+	<StructureSection load='6geh' size='340' side='right' caption='[[6geh]], [[Resolution\|resolution]] 1.15Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6geh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Shefn Shefn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GEH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GEH FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Sfri_2392 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=318167 SHEFN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6geh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6geh OCA], [http://pdbe.org/6geh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6geh RCSB], [http://www.ebi.ac.uk/pdbsum/6geh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6geh ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Siderophores make iron accessible under iron-limited conditions and play a crucial role in the survival of microorganisms. Because of their remarkable metal-scavenging properties and ease in crossing cellular envelopes, siderophores hold great potential in biotechnological applications, raising the need for a deeper knowledge of the molecular mechanisms underpinning the siderophore pathway. Here, we report the structural and functional characterization of a siderophore-interacting protein from the marine bacterium Shewanella frigidimarina NCIBM400 (SfSIP). SfSIP is a flavin-containing ferric-siderophore reductase with FAD- and NAD(P)H-binding domains that have high homology with other characterized SIPs. However, we found here that it mechanistically departs from what has been described for this family of proteins. Unlike other FAD-containing SIPs, SfSIP did not discriminate between NADH and NADPH. Furthermore, SfSIP required the presence of the Fe(2+)-scavenger, ferrozine, to use NAD(P)H to drive the reduction of Shewanella-produced hydroxamate ferric-siderophores. Additionally, this is the first SIP reported that also uses a ferredoxin as electron donor, and in contrast to NAD(P)H, its utilization did not require the mediation of ferrozine, and electron transfer occurred at fast rates. Finally, FAD oxidation was thermodynamically coupled to deprotonation at physiological pH values, enhancing the solubility of ferrous iron. On the basis of these results and the location of the SfSIP gene downstream of a sequence for putative binding of aerobic respiration control protein A (ArcA), we propose that SfSIP contributes an additional layer of regulation that maintains cellular iron homeostasis according to environmental cues of oxygen availability and cellular iron demand.

-	~~Authors: Trindade, I.B~~., Silva, ~~J.P.~~M., Matias~~, P.~~, Moe, E.	+	Structure and reactivity of a siderophore-interacting protein from the marine bacterium Shewanella reveals unanticipated functional versatility.,Trindade IB, Silva JM, Fonseca BM, Catarino T, Fujita M, Matias PM, Moe E, Louro RO J Biol Chem. 2019 Jan 4;294(1):157-167. doi: 10.1074/jbc.RA118.005041. Epub 2018 , Nov 12. PMID:30420426<ref>PMID:30420426</ref>

-	~~Description: Structure and reactivity of~~ a ~~siderophore-interacting protein from Shewanella reveals unanticipated functional versatility~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Moe, E~~]]	+	<div class="pdbe-citations 6geh" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Shefn]]
	[[Category: Matias, P]]		[[Category: Matias, P]]
-	[[Category: ~~Trindade~~, ~~I.B~~]]	+	[[Category: Moe, E]]
-	[[Category: Silva, J.P.M]]	+	[[Category: Silva, J P.M]]
		+	[[Category: Trindade, I B]]
		+	[[Category: Flavin binding]]
		+	[[Category: Metal transport]]
		+	[[Category: Rossmann fold]]

OCA: Protected "6geh" [edit=sysop:move=sysop]

2018-05-02T06:56:35Z

Protected "6geh" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:56, 2 May 2018

OCA: New page: '''Unreleased structure''' The entry 6geh is ON HOLD Authors: Trindade, I.B., Silva, J.P.M., Matias, P., Moe, E. Description: Structure and reactivity of a siderophore-interacting prot...

2018-05-02T06:56:34Z

New page: '''Unreleased structure''' The entry 6geh is ON HOLD Authors: Trindade, I.B., Silva, J.P.M., Matias, P., Moe, E. Description: Structure and reactivity of a siderophore-interacting prot...

New page

'''Unreleased structure'''

The entry 6geh is ON HOLD

Authors: Trindade, I.B., Silva, J.P.M., Matias, P., Moe, E.

Description: Structure and reactivity of a siderophore-interacting protein from Shewanella reveals unanticipated functional versatility
[[Category: Unreleased Structures]]
[[Category: Moe, E]]
[[Category: Matias, P]]
[[Category: Trindade, I.B]]
[[Category: Silva, J.P.M]]

6geh - Revision history

OCA at 12:33, 16 January 2019

OCA: Protected "6geh" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6geh is ON HOLD Authors: Trindade, I.B., Silva, J.P.M., Matias, P., Moe, E. Description: Structure and reactivity of a siderophore-interacting prot...