6gj2 - Revision history

OCA at 05:32, 8 June 2022

2022-06-08T05:32:38Z

←Older revision		Revision as of 05:32, 8 June 2022
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	<StructureSection load='6gj2' size='340' side='right'caption='[[6gj2]], [[Resolution\|resolution]] 1.68Å' scene=''>		<StructureSection load='6gj2' size='340' side='right'caption='[[6gj2]], [[Resolution\|resolution]] 1.68Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6gj2]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GJ2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6GJ2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6gj2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Canadian_hard_winter_wheat Canadian hard winter wheat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GJ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GJ2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=IHS:D-MYO-INOSITOL-HEXASULPHATE'>IHS</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=IHS:D-MYO-INOSITOL-HEXASULPHATE'>IHS</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6git\|6git]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6git\|6git]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PAPhy ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4565 Canadian hard winter wheat])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6gj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gj2 OCA], [~~http~~://pdbe.org/6gj2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6gj2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6gj2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6gj2 ProSAT]</span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gj2 OCA], [https://pdbe.org/6gj2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gj2 RCSB], [https://www.ebi.ac.uk/pdbsum/6gj2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gj2 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Grain phytate, a mixed metal ion salt of inositol hexakisphosphate, accounts for 60%-80% of stored phosphorus in plants and is a potent antinutrient of non-ruminant animals including humans. Through neofunctionalization of purple acid phytases (PAPhy), some cereals such as wheat and rye have acquired particularly high mature grain phytase activity. As PAPhy activity supplies phosphate, liberates metal ions necessary for seedling emergence, and obviates antinutrient effects of phytate, its manipulation and control are targeted crop traits. Here we show the X-ray crystal structure of the b2 isoform of wheat PAPhy induced during germination. This high-resolution crystal structure suggests a model for phytate recognition that, validated by molecular dynamics simulations, implicates elements of two sequence inserts (termed PAPhy motifs) relative to a canonical metallophosphoesterase (MPE) domain in forming phytate-specific substrate specificity pockets. These motifs are well conserved in PAPhys from monocot cereals, enzymes which are characterized by high specificity for phytate. Tested by mutagenesis, residues His229 in PAPhy motif 4 and Lys410 in the MPE domain, both conserved in PAPhys, are found to strongly influence phytase activity. These results explain the observed phytase activity of cereal PAPhys and open the way to the rational engineering of phytase activity in planta.
		+
		+	Structure of a cereal purple acid phytase provides new insights to phytate degradation in plants.,Faba-Rodriguez R, Gu Y, Salmon M, Dionisio G, Brinch-Pedersen H, Brearley CA, Hemmings AM Plant Commun. 2022 Feb 19;3(2):100305. doi: 10.1016/j.xplc.2022.100305., eCollection 2022 Mar 14. PMID:35529950<ref>PMID:35529950</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6gj2" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Acid phosphatase 3D structures\|Acid phosphatase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Acid phosphatase]]		[[Category: Acid phosphatase]]
		+	[[Category: Canadian hard winter wheat]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Brearley, C A]]		[[Category: Brearley, C A]]

OCA at 06:41, 27 November 2019

2019-11-27T06:41:02Z

←Older revision		Revision as of 06:41, 27 November 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6gj2 is ~~ON HOLD until Paper Publication~~	+	==PURPLE ACID PHYTASE FROM WHEAT ISOFORM B2 - COMPLEX WITH INOSITOL HEXASULPHATE==
-		+	<StructureSection load='6gj2' size='340' side='right'caption='[[6gj2]], [[Resolution\|resolution]] 1.68Å' scene=''>
-	~~Authors~~: ~~Faba~~-~~Rodriguez~~, R., ~~Brearley~~, C.A., ~~Hemmings~~, A.M.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6gj2]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GJ2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GJ2 FirstGlance]. <br>
-	~~Description: PURPLE ACID PHYTASE FROM WHEAT ISOFORM B2 -COMPLEX WITH INOSITOL HEXASULPHATE~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=IHS:D-MYO-INOSITOL-HEXASULPHATE'>IHS</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6git\|6git]]</td></tr>
-	[[Category: ~~Brearley, C.A~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span></td></tr>
-	[[Category: ~~Hemmings~~, A.M]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gj2 OCA], [http://pdbe.org/6gj2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gj2 RCSB], [http://www.ebi.ac.uk/pdbsum/6gj2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gj2 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Acid phosphatase]]
		+	[[Category: Large Structures]]
		+	[[Category: Brearley, C A]]
	[[Category: Faba-Rodriguez, R]]		[[Category: Faba-Rodriguez, R]]
		+	[[Category: Hemmings, A M]]
		+	[[Category: Ec 3 1.3 26]]
		+	[[Category: Hydrolase]]
		+	[[Category: Purple acid phytase]]

OCA at 04:11, 29 May 2019

2019-05-29T04:11:52Z

←Older revision		Revision as of 04:11, 29 May 2019
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	The entry 6gj2 is ON HOLD until Paper Publication		The entry 6gj2 is ON HOLD until Paper Publication

-	Authors: ~~FABA~~-~~RODRIGUEZ~~, R., ~~BREARLEY~~, C.A., ~~HEMMINGS~~, A.M.	+	Authors: Faba-Rodriguez, R., Brearley, C.A., Hemmings, A.M.

	Description: PURPLE ACID PHYTASE FROM WHEAT ISOFORM B2 -COMPLEX WITH INOSITOL HEXASULPHATE		Description: PURPLE ACID PHYTASE FROM WHEAT ISOFORM B2 -COMPLEX WITH INOSITOL HEXASULPHATE

OCA at 04:40, 24 April 2019

2019-04-24T04:40:01Z

←Older revision		Revision as of 04:40, 24 April 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6gj2 is ON HOLD	+	The entry 6gj2 is ON HOLD until Paper Publication

	Authors: FABA-RODRIGUEZ, R., BREARLEY, C.A., HEMMINGS, A.M.		Authors: FABA-RODRIGUEZ, R., BREARLEY, C.A., HEMMINGS, A.M.
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Brearley, C.A]]		[[Category: Brearley, C.A]]
-	[[Category: Faba-Rodriguez, R]]
	[[Category: Hemmings, A.M]]		[[Category: Hemmings, A.M]]
		+	[[Category: Faba-Rodriguez, R]]

OCA: Protected "6gj2" [edit=sysop:move=sysop]

2018-05-23T06:10:58Z

Protected "6gj2" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:10, 23 May 2018

OCA: New page: '''Unreleased structure''' The entry 6gj2 is ON HOLD Authors: FABA-RODRIGUEZ, R., BREARLEY, C.A., HEMMINGS, A.M. Description: PURPLE ACID PHYTASE FROM WHEAT ISOFORM B2 -COMPLEX WITH IN...

2018-05-23T06:10:57Z

New page: '''Unreleased structure''' The entry 6gj2 is ON HOLD Authors: FABA-RODRIGUEZ, R., BREARLEY, C.A., HEMMINGS, A.M. Description: PURPLE ACID PHYTASE FROM WHEAT ISOFORM B2 -COMPLEX WITH IN...

New page

'''Unreleased structure'''

The entry 6gj2 is ON HOLD

Authors: FABA-RODRIGUEZ, R., BREARLEY, C.A., HEMMINGS, A.M.

Description: PURPLE ACID PHYTASE FROM WHEAT ISOFORM B2 -COMPLEX WITH INOSITOL HEXASULPHATE
[[Category: Unreleased Structures]]
[[Category: Brearley, C.A]]
[[Category: Faba-Rodriguez, R]]
[[Category: Hemmings, A.M]]