6gms - Revision history

OCA at 10:48, 14 June 2023

2023-06-14T10:48:07Z

←Older revision		Revision as of 10:48, 14 June 2023
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	==Solution NMR structure of the major type IV pilin PpdD from enterohemorrhagic Escherichia coli (EHEC)==		==Solution NMR structure of the major type IV pilin PpdD from enterohemorrhagic Escherichia coli (EHEC)==
-	<StructureSection load='6gms' size='340' side='right'caption='[[6gms~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='6gms' size='340' side='right'caption='[[6gms]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6gms]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Eco57 Eco57~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GMS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6GMS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6gms]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GMS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GMS FirstGlance]. <br>
-	</td></tr>~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECs0112 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gms FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gms OCA], [https://pdbe.org/6gms PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gms RCSB], [https://www.ebi.ac.uk/pdbsum/6gms PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gms ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6gms FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gms OCA], [~~http~~://pdbe.org/6gms PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6gms RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6gms PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6gms ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q8X974_ECO57 Q8X974_ECO57]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Eco57~~]]	+	[[Category: Escherichia coli O157:H7]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Amorim~~, G C~~]]	+	[[Category: Amorim GC]]
-	[[Category: Bardiaux, B]]	+	[[Category: Bardiaux B]]
-	[[Category: Egelman, E]]	+	[[Category: Egelman E]]
-	[[Category: Francetic, O]]	+	[[Category: Francetic O]]
-	[[Category: Guilvout, I]]	+	[[Category: Guilvout I]]
-	[[Category: Izadi-Pruneyre, N]]	+	[[Category: Izadi-Pruneyre N]]
-	[[Category: Luna-Rico, A]]	+	[[Category: Luna-Rico A]]
-	[[Category: Nilges, M]]	+	[[Category: Nilges M]]
-	[[Category: Zeng, W]]	+	[[Category: Zeng W]]
-	~~[[Category: Cell adhesion]]~~	+
-	~~[[Category: Type iv pilin adhesion ehec t4p~~]]	+

OCA at 07:25, 21 May 2019

2019-05-21T07:25:11Z

←Older revision		Revision as of 07:25, 21 May 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6gms is ~~ON HOLD until Paper~~ Publication	+	==Solution NMR structure of the major type IV pilin PpdD from enterohemorrhagic Escherichia coli (EHEC)==
		+	<StructureSection load='6gms' size='340' side='right'caption='[[6gms]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6gms]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GMS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GMS FirstGlance]. <br>
		+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECs0112 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gms FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gms OCA], [http://pdbe.org/6gms PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gms RCSB], [http://www.ebi.ac.uk/pdbsum/6gms PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gms ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial type 4a pili are dynamic surface filaments that promote bacterial adherence, motility, and macromolecular transport. Their genes are highly conserved among enterobacteria and their expression in enterohemorrhagic Escherichia coli (EHEC) promotes adhesion to intestinal epithelia and pro-inflammatory signaling. To define the molecular basis of EHEC pilus assembly, we determined the structure of the periplasmic domain of its major subunit PpdD (PpdDp), a prototype of an enterobacterial pilin subfamily containing two disulfide bonds. The structure of PpdDp, determined by NMR, was then docked into the density envelope of purified EHEC pili obtained by cryoelectron microscopy (cryo-EM). Cryo-EM reconstruction of EHEC pili at approximately 8 A resolution revealed extremely high pilus flexibility correlating with a large extended region of the pilin stem. Systematic mutagenesis combined with functional and interaction analyses identified charged residues essential for pilus assembly. Structural information on exposed regions and interfaces between EHEC pilins is relevant for vaccine and drug discovery.

-	~~Authors: Amorim, G.C~~., Bardiaux, B., Luna-Rico, A~~., Zeng~~, W., Guilvout, I., ~~Egelman, E.~~, Nilges, M., ~~Francetic, O.~~, Izadi-Pruneyre, N.	+	Structure and Assembly of the Enterohemorrhagic Escherichia coli Type 4 Pilus.,Bardiaux B, de Amorim GC, Luna Rico A, Zheng W, Guilvout I, Jollivet C, Nilges M, Egelman EH, Izadi-Pruneyre N, Francetic O Structure. 2019 Apr 9. pii: S0969-2126(19)30117-0. doi:, 10.1016/j.str.2019.03.021. PMID:31056419<ref>PMID:31056419</ref>

-	~~Description: Solution NMR structure~~ of the ~~major type IV pilin PpdD from enterohemorrhagic Escherichia coli (EHEC)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6gms" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Eco57]]
		+	[[Category: Large Structures]]
		+	[[Category: Amorim, G C]]
		+	[[Category: Bardiaux, B]]
	[[Category: Egelman, E]]		[[Category: Egelman, E]]
-	[[Category: Zeng, W]]
-	[[Category: Amorim, G.C]]
-	[[Category: Izadi-Pruneyre, N]]
	[[Category: Francetic, O]]		[[Category: Francetic, O]]
-	[[Category: Nilges, M]]
	[[Category: Guilvout, I]]		[[Category: Guilvout, I]]
		+	[[Category: Izadi-Pruneyre, N]]
	[[Category: Luna-Rico, A]]		[[Category: Luna-Rico, A]]
-	[[Category: ~~Bardiaux~~, B]]	+	[[Category: Nilges, M]]
		+	[[Category: Zeng, W]]
		+	[[Category: Cell adhesion]]
		+	[[Category: Type iv pilin adhesion ehec t4p]]

OCA at 04:18, 20 June 2018

2018-06-20T04:18:41Z

←Older revision		Revision as of 04:18, 20 June 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6gms is ON HOLD	+	The entry 6gms is ON HOLD until Paper Publication

	Authors: Amorim, G.C., Bardiaux, B., Luna-Rico, A., Zeng, W., Guilvout, I., Egelman, E., Nilges, M., Francetic, O., Izadi-Pruneyre, N.		Authors: Amorim, G.C., Bardiaux, B., Luna-Rico, A., Zeng, W., Guilvout, I., Egelman, E., Nilges, M., Francetic, O., Izadi-Pruneyre, N.

OCA: Protected "6gms" [edit=sysop:move=sysop]

2018-06-06T04:29:34Z

Protected "6gms" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:29, 6 June 2018

OCA: New page: '''Unreleased structure''' The entry 6gms is ON HOLD Authors: Amorim, G.C., Bardiaux, B., Luna-Rico, A., Zeng, W., Guilvout, I., Egelman, E., Nilges, M., Francetic, O., Izadi-Pruneyre, ...

2018-06-06T04:29:33Z

New page: '''Unreleased structure''' The entry 6gms is ON HOLD Authors: Amorim, G.C., Bardiaux, B., Luna-Rico, A., Zeng, W., Guilvout, I., Egelman, E., Nilges, M., Francetic, O., Izadi-Pruneyre, ...

New page

'''Unreleased structure'''

The entry 6gms is ON HOLD

Authors: Amorim, G.C., Bardiaux, B., Luna-Rico, A., Zeng, W., Guilvout, I., Egelman, E., Nilges, M., Francetic, O., Izadi-Pruneyre, N.

Description: Solution NMR structure of the major type IV pilin PpdD from enterohemorrhagic Escherichia coli (EHEC)
[[Category: Unreleased Structures]]
[[Category: Egelman, E]]
[[Category: Zeng, W]]
[[Category: Amorim, G.C]]
[[Category: Izadi-Pruneyre, N]]
[[Category: Francetic, O]]
[[Category: Nilges, M]]
[[Category: Guilvout, I]]
[[Category: Luna-Rico, A]]
[[Category: Bardiaux, B]]