6gq6 - Revision history

OCA at 08:40, 10 October 2018

2018-10-10T08:40:14Z

←Older revision		Revision as of 08:40, 10 October 2018
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	<StructureSection load='6gq6' size='340' side='right' caption='[[6gq6]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='6gq6' size='340' side='right' caption='[[6gq6]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6gq6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GQ6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GQ6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6gq6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GQ6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GQ6 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6gp4\|6gp4]], [[6gpr\|6gpr]], [[6gpw\|6gpw]], [[6gpt\|6gpt]], [[6gpy\|6gpy]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6gp4\|6gp4]], [[6gpr\|6gpr]], [[6gpw\|6gpw]], [[6gpt\|6gpt]], [[6gpy\|6gpy]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HSP90AA1, HSP90A, HSPC1, HSPCA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gq6 OCA], [http://pdbe.org/6gq6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gq6 RCSB], [http://www.ebi.ac.uk/pdbsum/6gq6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gq6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gq6 OCA], [http://pdbe.org/6gq6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gq6 RCSB], [http://www.ebi.ac.uk/pdbsum/6gq6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gq6 ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Botta, M]]		[[Category: Botta, M]]
	[[Category: Mangani, S]]		[[Category: Mangani, S]]

OCA at 07:53, 3 October 2018

2018-10-03T07:53:15Z

←Older revision		Revision as of 07:53, 3 October 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6gq6 is ~~ON HOLD~~	+	==Structure of human Heat shock protein 90-alpha N-terminal domain (Hsp90-NTD) in complex with ADP==
		+	<StructureSection load='6gq6' size='340' side='right' caption='[[6gq6]], [[Resolution\|resolution]] 2.25Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6gq6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GQ6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GQ6 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6gp4\|6gp4]], [[6gpr\|6gpr]], [[6gpw\|6gpw]], [[6gpt\|6gpt]], [[6gpy\|6gpy]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gq6 OCA], [http://pdbe.org/6gq6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gq6 RCSB], [http://www.ebi.ac.uk/pdbsum/6gq6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gq6 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HS90A_HUMAN HS90A_HUMAN]] Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.<ref>PMID:15937123</ref> <ref>PMID:11274138</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In Brazil, the mucocutaneous form of leishmaniasis, caused by the parasite Leishmania braziliensis, is a widespread and very challenging disease responsible for disfiguration and, in the most severe cases, death. Heat shock protein 90 (Hsp90) is a ubiquitous molecular chaperone playing a pivotal role in the folding process of client proteins, and therefore its activity is fundamental for cell survival and proliferation. Since the chaperone activity requires ATP hydrolysis, molecules able to occupy the ATP binding pocket in the protein N-terminal domain (NTD) act as Hsp90 inhibitors. The development of selective molecules targeting the ATPase site of protozoan Hsp90 is tricky for the high homology with the human Hsp90 NTD (hNTD). Notably, only the human Lys112 is replaced by Arg97 in the L. braziliensis enzyme. Recently, this difference has been probed to design selective inhibitors targeting parasite Hsp90s. Here, a reliable protocol for expression and purification of LbHsp90-NTD (LbNTD) was developed but its structural characterization was unsuccessful. The role of Arg97 in LbNTD was hence probed by means of the "leishmanized" K112R variant of hNTDalpha. To deeply investigate the role of this residue, also the hNTDalpha K112A variant was generated. Structural studies performed on hNTDalpha and its variants using various ADP and ATP analogues and cAMP revealed that this residue is not crucial for nucleotide binding. This finding strongly suggests that Arg97 in LbNTD and more generally the conserved arginine residue in parasite Hsp90s are not exploitable for the development of selective inhibitors.

-	~~Authors: Tassone~~, G~~., Pozzi, C.~~, Mangani, S., Botta, M.	+	Probing the role of Arg97 in Heat shock protein 90 N-terminal domain from the parasite Leishmania braziliensis through site-directed mutagenesis on the human counterpart.,Tassone G, Mangani S, Botta M, Pozzi C Biochim Biophys Acta Proteins Proteom. 2018 Sep 21. pii: S1570-9639(18)30153-5., doi: 10.1016/j.bbapap.2018.09.005. PMID:30248409<ref>PMID:30248409</ref>

-	~~Description: Structure~~ of ~~human Heat shock protein 90~~-~~alpha N~~-~~terminal domain (Hsp90-NTD) in complex with ADP~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 6gq6" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Botta, M]]		[[Category: Botta, M]]
-	[[Category: Tassone, G]]
	[[Category: Mangani, S]]		[[Category: Mangani, S]]
	[[Category: Pozzi, C]]		[[Category: Pozzi, C]]
		+	[[Category: Tassone, G]]
		+	[[Category: Adp]]
		+	[[Category: Alpha]]
		+	[[Category: Chaperone]]
		+	[[Category: Complex]]
		+	[[Category: Hsp90]]
		+	[[Category: Ntd]]
		+	[[Category: Nucleotide]]

OCA: Protected "6gq6" [edit=sysop:move=sysop]

2018-06-14T06:00:39Z

Protected "6gq6" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:00, 14 June 2018

OCA: New page: '''Unreleased structure''' The entry 6gq6 is ON HOLD Authors: Tassone, G., Pozzi, C., Mangani, S., Botta, M. Description: Structure of human Heat shock protein 90-alpha N-terminal doma...

2018-06-14T06:00:37Z

New page: '''Unreleased structure''' The entry 6gq6 is ON HOLD Authors: Tassone, G., Pozzi, C., Mangani, S., Botta, M. Description: Structure of human Heat shock protein 90-alpha N-terminal doma...

New page

'''Unreleased structure'''

The entry 6gq6 is ON HOLD

Authors: Tassone, G., Pozzi, C., Mangani, S., Botta, M.

Description: Structure of human Heat shock protein 90-alpha N-terminal domain (Hsp90-NTD) in complex with ADP
[[Category: Unreleased Structures]]
[[Category: Botta, M]]
[[Category: Tassone, G]]
[[Category: Mangani, S]]
[[Category: Pozzi, C]]