6gx4 - Revision history

OCA at 08:24, 6 March 2019

2019-03-06T08:24:39Z

←Older revision		Revision as of 08:24, 6 March 2019
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	==The molybdenum storage protein: with ATP/Mn2+ and with POM clusters formed under in vitro conditions==		==The molybdenum storage protein: with ATP/Mn2+ and with POM clusters formed under in vitro conditions==
-	<StructureSection load='6gx4' size='340' side='right' caption='[[6gx4]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='6gx4' size='340' side='right' caption='[[6gx4]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6gx4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Azovd Azovd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GX4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6gx4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Azovd Azovd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GX4 FirstGlance]. <br>

OCA at 08:43, 26 December 2018

2018-12-26T08:43:16Z

←Older revision		Revision as of 08:43, 26 December 2018
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	<StructureSection load='6gx4' size='340' side='right' caption='[[6gx4]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='6gx4' size='340' side='right' caption='[[6gx4]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6gx4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Azotobacter_vinelandii_(strain_dj_/_atcc_baa-1303) Azotobacter vinelandii (strain dj / atcc baa-1303)~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GX4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6gx4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Azovd Azovd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GX4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=FUQ:Mo5+Cluster'>FUQ</scene>, <scene name='pdbligand=FV2:Mo8+cluster'>FV2</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=FUQ:Mo5+Cluster'>FUQ</scene>, <scene name='pdbligand=FV2:Mo8+cluster'>FV2</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gx4 OCA], [http://pdbe.org/6gx4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gx4 RCSB], [http://www.ebi.ac.uk/pdbsum/6gx4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gx4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gx4 OCA], [http://pdbe.org/6gx4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gx4 RCSB], [http://www.ebi.ac.uk/pdbsum/6gx4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gx4 ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Azovd]]
	[[Category: Bruenle, S]]		[[Category: Bruenle, S]]
	[[Category: Ermler, E]]		[[Category: Ermler, E]]

OCA at 06:41, 5 December 2018

2018-12-05T06:41:47Z

←Older revision		Revision as of 06:41, 5 December 2018
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-	'''Unreleased structure'''

-	The ~~entry~~ 6gx4 is ~~ON HOLD~~	+	==The molybdenum storage protein: with ATP/Mn2+ and with POM clusters formed under in vitro conditions==
		+	<StructureSection load='6gx4' size='340' side='right' caption='[[6gx4]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6gx4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Azotobacter_vinelandii_(strain_dj_/_atcc_baa-1303) Azotobacter vinelandii (strain dj / atcc baa-1303)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GX4 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=FUQ:Mo5+Cluster'>FUQ</scene>, <scene name='pdbligand=FV2:Mo8+cluster'>FV2</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gx4 OCA], [http://pdbe.org/6gx4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gx4 RCSB], [http://www.ebi.ac.uk/pdbsum/6gx4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gx4 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MOSB_AZOVD MOSB_AZOVD]] Intracellular storage of molybdenum. Binds polyoxomolybdates. Can bind at least 90 molybdenum atoms per protein molecule. [[http://www.uniprot.org/uniprot/MOSA_AZOVD MOSA_AZOVD]] Intracellular storage of molybdenum. Binds polyoxomolybdates. Can bind at least 90 molybdenum atoms per protein molecule.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A continuous FeMo-cofactor supply for nitrogenase maturation is ensured in Azotobacter vinelandii by developing a cage-like molybdenum storage protein (MoSto) capable to store ca. 120 molybdate molecules (MoO4 (2-) ) as discrete polyoxometalate (POM) clusters. To gain mechanistic insight into this process MoSto was characterized by Mo and ATP/ADP content, structural and kinetic analysis. We defined three functionally relevant states specified by the presence of both ATP/ADP and POM clusters (MoStofunct ), of only ATP/ADP (MoStobasal ) and of neither ATP/ADP nor POM clusters (MoStozero ), respectively. POM clusters are only produced when ATP is hydrolyzed to ADP and phosphate. Vmax was ca. 13 mumolphosphate min(-1) mg(-1) and Km for molybdate and ATP/Mg(2+) in the low micromolar range. ATP hydrolysis presumably proceeds at subunit alpha, which is compatible with a highly occupied alpha-ATP/Mg(2+) and a weaker occupied beta-ATP/no Mg(2+) binding site found in the MoStofunct structure. Several findings indicate that POM cluster storage is separated into a rapid ATP-hydrolysis dependent molybdate transport across the protein cage wall and a slow molybdate assembly induced by combined auto-catalytic and protein-driven processes. The cage interior, the location of the POM cluster depot, is locked in all three states and thus not rapidly accessible for molybdate from the outside. Based on Vmax the entire Mo storage process should be completed in less than 10 s but requires, according to the molybdate content analysis, ca. 15 min. Long-time incubation of MoStobasal with non-physiological high molybdate amounts implicates an equilibrium in and outside the cage and POM cluster self-formation without ATP hydrolysis. This article is protected by copyright. All rights reserved.

-	~~Authors~~: ~~Poppe~~, J~~., Bruenle~~, S., Hail, R., ~~Ermler~~, E.	+	The Molybdenum Storage Protein: A soluble ATP hydrolysis dependent molybdate pump.,Poppe J, Brunle S, Hail R, Wiesemann K, Schneider K, Ermler U FEBS J. 2018 Oct 27. doi: 10.1111/febs.14684. PMID:30367742<ref>PMID:30367742</ref>

-	~~Description: The molybdenum storage protein: with ATP~~/~~Mn2+ and with POM clusters formed under in vitro conditions~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Poppe~~, J]]	+	<div class="pdbe-citations 6gx4" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bruenle, S]]
	[[Category: Ermler, E]]		[[Category: Ermler, E]]
	[[Category: Hail, R]]		[[Category: Hail, R]]
-	[[Category: ~~Bruenle~~, S]]	+	[[Category: Poppe, J]]
		+	[[Category: Atp consumption]]
		+	[[Category: Metal binding protein]]
		+	[[Category: Molybdate storage]]
		+	[[Category: Polyoxometalate cluster]]

OCA at 18:27, 2 December 2018

2018-12-02T18:27:25Z

←Older revision		Revision as of 18:27, 2 December 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6gx4 is ON HOLD ~~until sometime in the future~~	+	The entry 6gx4 is ON HOLD

	Authors: Poppe, J., Bruenle, S., Hail, R., Ermler, E.		Authors: Poppe, J., Bruenle, S., Hail, R., Ermler, E.
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	Description: The molybdenum storage protein: with ATP/Mn2+ and with POM clusters formed under in vitro conditions		Description: The molybdenum storage protein: with ATP/Mn2+ and with POM clusters formed under in vitro conditions
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: ~~Hail~~, R]]	+	[[Category: Poppe, J]]
	[[Category: Ermler, E]]		[[Category: Ermler, E]]
		+	[[Category: Hail, R]]
	[[Category: Bruenle, S]]		[[Category: Bruenle, S]]
-	[[Category: Poppe, J]]

OCA at 04:14, 11 July 2018

2018-07-11T04:14:37Z

←Older revision		Revision as of 04:14, 11 July 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6gx4 is ON HOLD	+	The entry 6gx4 is ON HOLD until sometime in the future

	Authors: Poppe, J., Bruenle, S., Hail, R., Ermler, E.		Authors: Poppe, J., Bruenle, S., Hail, R., Ermler, E.

OCA: Protected "6gx4" [edit=sysop:move=sysop]

2018-07-04T05:55:51Z

Protected "6gx4" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:55, 4 July 2018

OCA: New page: '''Unreleased structure''' The entry 6gx4 is ON HOLD Authors: Poppe, J., Bruenle, S., Hail, R., Ermler, E. Description: The molybdenum storage protein: with ATP/Mn2+ and with POM clust...

2018-07-04T05:55:50Z

New page: '''Unreleased structure''' The entry 6gx4 is ON HOLD Authors: Poppe, J., Bruenle, S., Hail, R., Ermler, E. Description: The molybdenum storage protein: with ATP/Mn2+ and with POM clust...

New page

'''Unreleased structure'''

The entry 6gx4 is ON HOLD

Authors: Poppe, J., Bruenle, S., Hail, R., Ermler, E.

Description: The molybdenum storage protein: with ATP/Mn2+ and with POM clusters formed under in vitro conditions
[[Category: Unreleased Structures]]
[[Category: Hail, R]]
[[Category: Ermler, E]]
[[Category: Bruenle, S]]
[[Category: Poppe, J]]