6h01 - Revision history

OCA at 07:25, 26 June 2019

2019-06-26T07:25:40Z

←Older revision		Revision as of 07:25, 26 June 2019
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	<StructureSection load='6h01' size='340' side='right'caption='[[6h01]], [[Resolution\|resolution]] 2.78Å' scene=''>		<StructureSection load='6h01' size='340' side='right'caption='[[6h01]], [[Resolution\|resolution]] 2.78Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6h01]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H01 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H01 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6h01]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H01 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H01 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FHE:'>FHE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FHE:'>FHE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gfp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h01 OCA], [http://pdbe.org/6h01 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h01 RCSB], [http://www.ebi.ac.uk/pdbsum/6h01 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h01 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h01 OCA], [http://pdbe.org/6h01 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h01 RCSB], [http://www.ebi.ac.uk/pdbsum/6h01 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h01 ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aeqvi]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Kesgin-Schaefer, S]]		[[Category: Kesgin-Schaefer, S]]

OCA at 06:47, 24 April 2019

2019-04-24T06:47:29Z

←Older revision		Revision as of 06:47, 24 April 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6h01 is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of a domain-swapped dark-state sfGFP containing the unnatural amino acid ortho-nitrobenzyl-tyrosine (ONBY) at residue 66==
		+	<StructureSection load='6h01' size='340' side='right'caption='[[6h01]], [[Resolution\|resolution]] 2.78Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6h01]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H01 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H01 FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FHE:'>FHE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h01 OCA], [http://pdbe.org/6h01 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h01 RCSB], [http://www.ebi.ac.uk/pdbsum/6h01 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h01 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Photoactivatable fluorescent proteins (PA-FPs) are a powerful non-invasive tool in high-resolution live-cell imaging. They can be converted from an inactive to an active form by light, enabling the spatial and temporal trafficking of proteins and cell dynamics. PA-FPs have been previously generated by mutating selected residues in the chromophore or in its close proximity. A new strategy to generate PA-FPs is the genetic incorporation of unnatural amino acids (UAAs) containing photocaged groups using unique suppressor tRNA/aminoacyl-tRNA synthetase pairs. We set out to develop a photoactivatable GFP variant suitable for time-resolved structural studies. Here, we report the crystal structure of superfolder GFP (sfGFP) containing the UAA ortho-nitrobenzyl-tyrosine (ONBY) at position 66 and its spectroscopic characterization. Surprisingly, the crystal structure (to 2.7 A resolution) reveals a dimeric domain-swapped arrangement of sfGFP66ONBY with residues 1-142 of one molecule associating with residues 148-234 from another molecule. This unusual domain-swapped structure supports a previously postulated GFP folding pathway that proceeds via an equilibrium intermediate.

-	~~Authors:~~ Kesgin-Schaefer, S., ~~Tidow~~, H.	+	Crystal structure of a domain-swapped photoactivatable sfGFP variant provides evidence for GFP folding pathway.,Kesgin-Schaefer S, Heidemann J, Puchert A, Koelbel K, Yorke BA, Huse N, Pearson AR, Uetrecht C, Tidow H FEBS J. 2019 Feb 28. doi: 10.1111/febs.14797. PMID:30817081<ref>PMID:30817081</ref>

-	~~Description: Crystal structure of~~ a ~~domain-swapped dark-state sfGFP containing~~ the ~~unnatural amino acid ortho~~-~~nitrobenzyl~~-~~tyrosine (ONBY) at residue 66~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6h01" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Kesgin-Schaefer, S]]		[[Category: Kesgin-Schaefer, S]]
	[[Category: Tidow, H]]		[[Category: Tidow, H]]
		+	[[Category: Domain-swapped]]
		+	[[Category: Fluorescent protein]]
		+	[[Category: Gfp]]
		+	[[Category: Unnatural amino acid]]

OCA at 04:51, 22 August 2018

2018-08-22T04:51:09Z

←Older revision		Revision as of 04:51, 22 August 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6h01 is ON HOLD	+	The entry 6h01 is ON HOLD until Paper Publication

	Authors: Kesgin-Schaefer, S., Tidow, H.		Authors: Kesgin-Schaefer, S., Tidow, H.

OCA: Protected "6h01" [edit=sysop:move=sysop]

2018-07-11T04:20:51Z

Protected "6h01" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:20, 11 July 2018

OCA: New page: '''Unreleased structure''' The entry 6h01 is ON HOLD Authors: Kesgin-Schaefer, S., Tidow, H. Description: Crystal structure of a domain-swapped dark-state sfGFP containing the unnatura...

2018-07-11T04:20:50Z

New page: '''Unreleased structure''' The entry 6h01 is ON HOLD Authors: Kesgin-Schaefer, S., Tidow, H. Description: Crystal structure of a domain-swapped dark-state sfGFP containing the unnatura...

New page

'''Unreleased structure'''

The entry 6h01 is ON HOLD

Authors: Kesgin-Schaefer, S., Tidow, H.

Description: Crystal structure of a domain-swapped dark-state sfGFP containing the unnatural amino acid ortho-nitrobenzyl-tyrosine (ONBY) at residue 66
[[Category: Unreleased Structures]]
[[Category: Kesgin-Schaefer, S]]
[[Category: Tidow, H]]