6h53 - Revision history

OCA at 06:34, 29 May 2019

2019-05-29T06:34:59Z

←Older revision		Revision as of 06:34, 29 May 2019
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h53 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h53 OCA], [http://pdbe.org/6h53 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h53 RCSB], [http://www.ebi.ac.uk/pdbsum/6h53 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h53 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h53 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h53 OCA], [http://pdbe.org/6h53 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h53 RCSB], [http://www.ebi.ac.uk/pdbsum/6h53 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h53 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Tuberculosis causes over one million yearly deaths, and drug resistance is rapidly developing. Mycobacterium tuberculosis phosphatidylinositol phosphate synthase (PgsA1) is an integral membrane enzyme involved in biosynthesis of inositol-derived phospholipids required for formation of the mycobacterial cell wall, and a potential drug target. Here we present three crystal structures of M. tuberculosis PgsA1: in absence of substrates (2.9 A), in complex with Mn(2+) and citrate (1.9 A), and with the CDP-DAG substrate (1.8 A). The structures reveal atomic details of substrate binding as well as coordination and dynamics of the catalytic metal site. In addition, molecular docking supported by mutagenesis indicate a binding mode for the second substrate, D-myo-inositol-3-phosphate. Together, the data describe the structural basis for M. tuberculosis phosphatidylinositol phosphate synthesis and suggest a refined general catalytic mechanism-including a substrate-induced carboxylate shift-for Class I CDP-alcohol phosphotransferases, enzymes essential for phospholipid biosynthesis in all domains of life.
		+
		+	Structure of Mycobacterium tuberculosis phosphatidylinositol phosphate synthase reveals mechanism of substrate binding and metal catalysis.,Grave K, Bennett MD, Hogbom M Commun Biol. 2019 May 8;2:175. doi: 10.1038/s42003-019-0427-1. eCollection 2019. PMID:31098408<ref>PMID:31098408</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6h53" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:26, 21 May 2019

2019-05-21T07:26:09Z

←Older revision		Revision as of 07:26, 21 May 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6h53 is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of Mycobacterium tuberculosis phosphatidylinositol phosphate synthase (PgsA1) in apo form==
-		+	<StructureSection load='6h53' size='340' side='right'caption='[[6h53]], [[Resolution\|resolution]] 2.90Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6h53]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H53 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H53 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UPL:UNKNOWN+BRANCHED+FRAGMENT+OF+PHOSPHOLIPID'>UPL</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pgsA1, Rv2612c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/CDP-diacylglycerol--inositol_3-phosphatidyltransferase CDP-diacylglycerol--inositol 3-phosphatidyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.8.11 2.7.8.11] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h53 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h53 OCA], [http://pdbe.org/6h53 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h53 RCSB], [http://www.ebi.ac.uk/pdbsum/6h53 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h53 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: CDP-diacylglycerol--inositol 3-phosphatidyltransferase]]
		+	[[Category: Large Structures]]
		+	[[Category: Myctu]]
		+	[[Category: Grave, K]]
		+	[[Category: Hogbom, M]]
		+	[[Category: Phosphotransferase glycerophospholipid metabolism metal binding protein]]
		+	[[Category: Transferase]]

OCA at 20:03, 9 August 2018

2018-08-09T20:03:09Z

←Older revision		Revision as of 20:03, 9 August 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6h53 is ON HOLD	+	The entry 6h53 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6h53" [edit=sysop:move=sysop]

2018-08-01T17:16:20Z

Protected "6h53" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:16, 1 August 2018

OCA: New page: '''Unreleased structure''' The entry 6h53 is ON HOLD Authors: Description: Category: Unreleased Structures

2018-08-01T17:16:19Z

New page: '''Unreleased structure''' The entry 6h53 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6h53 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]