OCA at 07:57, 24 April 2019

2019-04-24T07:57:05Z

←Older revision		Revision as of 07:57, 24 April 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6hai is ~~ON HOLD until Paper~~ Publication	+	==AlbAM131A mutant in complex with albicidin , albicidin resistance protein==
		+	<StructureSection load='6hai' size='340' side='right'caption='[[6hai]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6hai]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_oxytocus_perniciosus"_flugge_1886 "bacillus oxytocus perniciosus" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HAI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HAI FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FWW:4-[[4-[[4-[(3~{S})-3-[[4-[[(~{E})-3-(4-hydroxyphenyl)-2-methyl-prop-2-enoyl]amino]phenyl]carbonylamino]-2,5-bis(oxidanylidene)pyrrolidin-1-yl]phenyl]carbonylamino]-3-methoxy-2-oxidanyl-phenyl]carbonylamino]-3-methoxy-2-oxidanyl-benzoic+acid'>FWW</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">albA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=571 "Bacillus oxytocus perniciosus" Flugge 1886])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hai FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hai OCA], [http://pdbe.org/6hai PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hai RCSB], [http://www.ebi.ac.uk/pdbsum/6hai PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hai ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	To combat the rise of antimicrobial resistance, the discovery of new antibiotics is paramount. Albicidin and cystobactamid are related natural product antibiotics with potent activity against Gram-positive and, crucially, Gram-negative pathogens. AlbA has been reported to neutralize albicidin by binding it with nanomolar affinity. To understand this potential resistance mechanism, we determined structures of AlbA and its complex with albicidin. The structures revealed AlbA to be comprised of two domains, each unexpectedly resembling the multiantibiotic neutralizing protein TipA. Binding of the long albicidin molecule was shared pseudosymmetrically between the two domains. The structure also revealed an unexpected chemical modification of albicidin, which we demonstrate to be promoted by AlbA, and to reduce albicidin potency; we propose a mechanism for this reaction. Overall, our findings suggest that AlbA arose through internal duplication in an ancient TipA-like gene, leading to a new binding scaffold adapted to the sequestration of long-chain antibiotics.

-	~~Authors: Koehnke, J~~., Sikandar, A.	+	Adaptation of a Bacterial Multidrug Resistance System Revealed by the Structure and Function of AlbA.,Sikandar A, Cirnski K, Testolin G, Volz C, Bronstrup M, Kalinina OV, Muller R, Koehnke J J Am Chem Soc. 2018 Dec 5;140(48):16641-16649. doi: 10.1021/jacs.8b08895. Epub, 2018 Nov 27. PMID:30422653<ref>PMID:30422653</ref>

-	~~Description: AlbAM133A mutant in complex with albicidin~~ , ~~albicidin resistance protein~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Sikandar, A~~]]	+	<div class="pdbe-citations 6hai" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bacillus oxytocus perniciosus flugge 1886]]
		+	[[Category: Large Structures]]
	[[Category: Koehnke, J]]		[[Category: Koehnke, J]]
		+	[[Category: Sikandar, A]]
		+	[[Category: Albicidin binding protein]]
		+	[[Category: Albicidin resistance protein]]

OCA at 04:53, 22 August 2018

2018-08-22T04:53:18Z

←Older revision		Revision as of 04:53, 22 August 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6hai is ON HOLD until ~~sometime in the future~~	+	The entry 6hai is ON HOLD until Paper Publication

	Authors: Koehnke, J., Sikandar, A.		Authors: Koehnke, J., Sikandar, A.

OCA: Protected "6hai" [edit=sysop:move=sysop]

2018-08-15T15:02:58Z

Protected "6hai" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:02, 15 August 2018

OCA: New page: '''Unreleased structure''' The entry 6hai is ON HOLD until sometime in the future Authors: Koehnke, J., Sikandar, A. Description: AlbAM133A mutant in complex with albicidin , albicidin...

2018-08-15T15:02:56Z

New page: '''Unreleased structure''' The entry 6hai is ON HOLD until sometime in the future Authors: Koehnke, J., Sikandar, A. Description: AlbAM133A mutant in complex with albicidin , albicidin...

New page

'''Unreleased structure'''

The entry 6hai is ON HOLD until sometime in the future

Authors: Koehnke, J., Sikandar, A.

Description: AlbAM133A mutant in complex with albicidin , albicidin resistance protein
[[Category: Unreleased Structures]]
[[Category: Sikandar, A]]
[[Category: Koehnke, J]]

6hai - Revision history

OCA at 07:57, 24 April 2019

OCA at 04:53, 22 August 2018

OCA: Protected "6hai" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6hai is ON HOLD until sometime in the future Authors: Koehnke, J., Sikandar, A. Description: AlbAM133A mutant in complex with albicidin , albicidin...