OCA at 11:31, 13 November 2019

2019-11-13T11:31:59Z

←Older revision		Revision as of 11:31, 13 November 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6hpb is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of the E.coli HicAB toxin-antitoxin complex==
		+	<StructureSection load='6hpb' size='340' side='right'caption='[[6hpb]], [[Resolution\|resolution]] 2.28Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6hpb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_2-222-05_s4_c3 Escherichia coli 2-222-05_s4_c3] and [http://en.wikipedia.org/wiki/Escherichia_coli_mg1655 Escherichia coli mg1655]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HPB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HPB FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6hpc\|6hpc]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hicA, yncN, b4532, JW5230 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511145 Escherichia coli MG1655]), hicB, ydcQ, b1438, JW1433 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1444264 Escherichia coli 2-222-05_S4_C3])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hpb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hpb OCA], [http://pdbe.org/6hpb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hpb RCSB], [http://www.ebi.ac.uk/pdbsum/6hpb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hpb ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HICA_ECOLI HICA_ECOLI]] Toxic component of a type II toxin-antitoxin (TA) system. A probable translation-independent mRNA interferase. Overexpression causes cessation of cell growth and inhibits cell proliferation via inhibition of translation; this blockage is overcome (after 90 minutes) by subsequent expression of antitoxin HicB. Overexpression causes cleavage of a number of mRNAs and tmRNA, in a translation-independent fashion, suggesting this is an mRNA interferase (PubMed:19060138). mRNA interferases play a role in bacterial persistence to antibiotics (PubMed:21788497).<ref>PMID:19060138</ref> <ref>PMID:21788497</ref> [[http://www.uniprot.org/uniprot/HICB_ECOLI HICB_ECOLI]] Antitoxin component of a type II toxin-antitoxin (TA) system. Functions as an mRNA interferase antitoxin; overexpression prevents HicA-mediated cessation of cell growth and inhibition of cell proliferation.<ref>PMID:19060138</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The E. coli hicAB type II toxin-antitoxin locus is unusual by being controlled by two promoters and by having the toxin encoded upstream of the antitoxin. HicA toxins contain a double-stranded RNA-binding fold and cleaves both mRNA and tmRNA in vivo, while HicB antitoxins contain a partial RNase H fold and either a helix-turn-helix (HTH) or ribbon-helix-helix domain. It is not known how an HTH DNA-binding domain affects higher-order structure for the HicAB modules. Here, we present crystal structures of the isolated E. coli HicB antitoxin and full-length HicAB complex showing that HicB forms a stable DNA-binding module and interacts in a canonical way with HicA despite the presence of an HTH-type DNA-binding domain. No major structural rearrangements take place upon binding of the toxin. Both structures expose well-ordered DNA-binding motifs allowing a model for DNA binding by the antitoxin to be generated.

-	~~Authors~~:	+	The E. coli HicB Antitoxin Contains a Structurally Stable Helix-Turn-Helix DNA Binding Domain.,Manav MC, Turnbull KJ, Jurenas D, Garcia-Pino A, Gerdes K, Brodersen DE Structure. 2019 Sep 4. pii: S0969-2126(19)30279-5. doi:, 10.1016/j.str.2019.08.008. PMID:31495532<ref>PMID:31495532</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6hpb" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia coli 2-222-05_s4_c3]]
		+	[[Category: Escherichia coli mg1655]]
		+	[[Category: Large Structures]]
		+	[[Category: Brodersen, D E]]
		+	[[Category: Manav, M C]]
		+	[[Category: Dna-binding]]
		+	[[Category: Protein complex]]
		+	[[Category: Ta]]
		+	[[Category: Ta complex]]
		+	[[Category: Toxin]]
		+	[[Category: Toxin-antitoxin]]

OCA at 06:12, 3 October 2018

2018-10-03T06:12:49Z

←Older revision		Revision as of 06:12, 3 October 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6hpb is ON HOLD	+	The entry 6hpb is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6hpb" [edit=sysop:move=sysop]

2018-09-26T06:17:27Z

Protected "6hpb" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:17, 26 September 2018

OCA: New page: '''Unreleased structure''' The entry 6hpb is ON HOLD Authors: Description: Category: Unreleased Structures

2018-09-26T06:17:25Z

New page: '''Unreleased structure''' The entry 6hpb is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6hpb is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6hpb - Revision history

OCA at 11:31, 13 November 2019

OCA at 06:12, 3 October 2018

OCA: Protected "6hpb" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6hpb is ON HOLD Authors: Description: Category: Unreleased Structures