6hua - Revision history

OCA at 11:39, 24 January 2024

2024-01-24T11:39:45Z

←Older revision		Revision as of 11:39, 24 January 2024
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	<StructureSection load='6hua' size='340' side='right'caption='[[6hua]], [[Resolution\|resolution]] 3.39Å' scene=''>		<StructureSection load='6hua' size='340' side='right'caption='[[6hua]], [[Resolution\|resolution]] 3.39Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6hua]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Streptococcus_vestibularis_f0396~~ Streptococcus vestibularis ~~f0396~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HUA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6HUA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6hua]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_vestibularis_F0396 Streptococcus vestibularis F0396]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HUA FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~HMPREF9192_0126 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=904306 Streptococcus vestibularis F0396])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.389Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6hua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hua OCA], [~~http~~://pdbe.org/6hua PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6hua RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6hua PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6hua ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6hua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hua OCA], [https://pdbe.org/6hua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6hua RCSB], [https://www.ebi.ac.uk/pdbsum/6hua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6hua ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/E3CNF6_STRVE E3CNF6_STRVE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 16:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 6hua" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6hua" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Transcriptional activator 3D structures\|Transcriptional activator 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 21:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Streptococcus vestibularis ~~f0396~~]]	+	[[Category: Streptococcus vestibularis F0396]]
-	[[Category: Hols, P]]	+	[[Category: Hols P]]
-	[[Category: Ledesma, L]]	+	[[Category: Ledesma L]]
-	[[Category: Nessler, S]]	+	[[Category: Nessler S]]
-	[[Category: Thuillier, J]]	+	[[Category: Thuillier J]]
-	~~[[Category: Rnpp family tpr domain hth domain bacterial signaling peptide binding]]~~	+
-	~~[[Category: Transcription~~]]	+

OCA at 05:59, 22 April 2020

2020-04-22T05:59:29Z

←Older revision		Revision as of 05:59, 22 April 2020
Line 3:		Line 3:
	<StructureSection load='6hua' size='340' side='right'caption='[[6hua]], [[Resolution\|resolution]] 3.39Å' scene=''>		<StructureSection load='6hua' size='340' side='right'caption='[[6hua]], [[Resolution\|resolution]] 3.39Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6hua]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_vestibularis_f0396 Streptococcus vestibularis f0396]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HUA OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6HUA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6hua]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_vestibularis_f0396 Streptococcus vestibularis f0396]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HUA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6HUA FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HMPREF9192_0126 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=904306 Streptococcus vestibularis F0396])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HMPREF9192_0126 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=904306 Streptococcus vestibularis F0396])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6hua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hua OCA], [http://pdbe.org/6hua PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hua RCSB], [http://www.ebi.ac.uk/pdbsum/6hua PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hua ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6hua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hua OCA], [http://pdbe.org/6hua PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hua RCSB], [http://www.ebi.ac.uk/pdbsum/6hua PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hua ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 09:28, 1 April 2020

2020-04-01T09:28:44Z

←Older revision		Revision as of 09:28, 1 April 2020
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	<StructureSection load='6hua' size='340' side='right'caption='[[6hua]], [[Resolution\|resolution]] 3.39Å' scene=''>		<StructureSection load='6hua' size='340' side='right'caption='[[6hua]], [[Resolution\|resolution]] 3.39Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6hua]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HUA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HUA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6hua]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_vestibularis_f0396 Streptococcus vestibularis f0396]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HUA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HUA FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hua OCA], [http://pdbe.org/6hua PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hua RCSB], [http://www.ebi.ac.uk/pdbsum/6hua PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hua ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HMPREF9192_0126 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=904306 Streptococcus vestibularis F0396])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hua OCA], [http://pdbe.org/6hua PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hua RCSB], [http://www.ebi.ac.uk/pdbsum/6hua PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hua ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Competence allows bacteria to internalize exogenous DNA fragments for the acquisition of new phenotypes such as antibiotic resistance or virulence traits. In most streptococci, competence is regulated by ComRS signaling, a system based on the mature ComS pheromone (XIP), which is internalized to activate the (R)RNPP-type ComR sensor by triggering dimerization and DNA binding. Cross-talk analyses demonstrated major differences of selectivity between ComRS systems and raised questions concerning the mechanism of pheromone-sensor recognition and coevolution. Here, we decipher the molecular determinants of selectivity of the closely related ComRS systems from Streptococcus thermophilus and Streptococcus vestibularis Despite high similarity, we show that the divergence in ComR-XIP interaction does not allow reciprocal activation. We perform the structural analysis of the ComRS system from S. vestibularis. Comparison with its ortholog from S. thermophilus reveals an activation mechanism based on a toggle switch involving the recruitment of a key loop by the XIP C terminus. Together with a broad mutational analysis, we identify essential residues directly involved in peptide binding. Notably, we generate a ComR mutant that displays a fully reversed selectivity toward the heterologous pheromone with only five point mutations, as well as other ComR variants featuring XIP bispecificity and/or neofunctionalization for hybrid XIP peptides. We also reveal that a single XIP mutation relaxes the strictness of ComR activation, suggesting fast adaptability of molecular communication phenotypes. Overall, this study is paving the way toward the rational design or directed evolution of artificial ComRS systems for a range of biotechnological and biomedical applications.
		+
		+	Molecular dissection of pheromone selectivity in the competence signaling system ComRS of streptococci.,Ledesma-Garcia L, Thuillier J, Guzman-Espinola A, Ensinck I, Li de la Sierra-Gallay I, Lazar N, Aumont-Nicaise M, Mignolet J, Soumillion P, Nessler S, Hols P Proc Natl Acad Sci U S A. 2020 Mar 20. pii: 1916085117. doi:, 10.1073/pnas.1916085117. PMID:32198205<ref>PMID:32198205</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6hua" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Streptococcus vestibularis f0396]]
	[[Category: Hols, P]]		[[Category: Hols, P]]
	[[Category: Ledesma, L]]		[[Category: Ledesma, L]]

OCA at 06:45, 23 October 2019

2019-10-23T06:45:54Z

←Older revision		Revision as of 06:45, 23 October 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6hua is ON HOLD until Paper Publication~~	+	==the competence regulator ComR from Streptococcus vestibularis in complex with its cognate signaling peptide XIP==
-		+	<StructureSection load='6hua' size='340' side='right'caption='[[6hua]], [[Resolution\|resolution]] 3.39Å' scene=''>
-	~~Authors: Nessler, S., Thuillier, J., Ledesma, L., Hols, P.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6hua]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HUA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HUA FirstGlance]. <br>
-	~~Description:~~ the competence regulator ComR from Streptococcus vestibularis in complex with its cognate signaling peptide XIP	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hua OCA], [http://pdbe.org/6hua PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hua RCSB], [http://www.ebi.ac.uk/pdbsum/6hua PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hua ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Thuillier~~, J]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Hols, P]]		[[Category: Hols, P]]
		+	[[Category: Ledesma, L]]
	[[Category: Nessler, S]]		[[Category: Nessler, S]]
-	[[Category: ~~Ledesma~~, L]]	+	[[Category: Thuillier, J]]
		+	[[Category: Rnpp family tpr domain hth domain bacterial signaling peptide binding]]
		+	[[Category: Transcription]]

OCA at 04:14, 16 October 2019

2019-10-16T04:14:20Z

←Older revision		Revision as of 04:14, 16 October 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6hua is ON HOLD	+	The entry 6hua is ON HOLD until Paper Publication

	Authors: Nessler, S., Thuillier, J., Ledesma, L., Hols, P.		Authors: Nessler, S., Thuillier, J., Ledesma, L., Hols, P.
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	[[Category: Thuillier, J]]		[[Category: Thuillier, J]]
	[[Category: Hols, P]]		[[Category: Hols, P]]
-	[[Category: Ledesma, L]]
	[[Category: Nessler, S]]		[[Category: Nessler, S]]
		+	[[Category: Ledesma, L]]

OCA: Protected "6hua" [edit=sysop:move=sysop]

2018-10-17T06:14:31Z

Protected "6hua" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:14, 17 October 2018

OCA: New page: '''Unreleased structure''' The entry 6hua is ON HOLD Authors: Nessler, S., Thuillier, J., Ledesma, L., Hols, P. Description: the competence regulator ComR from Streptococcus vestibular...

2018-10-17T06:14:30Z

New page: '''Unreleased structure''' The entry 6hua is ON HOLD Authors: Nessler, S., Thuillier, J., Ledesma, L., Hols, P. Description: the competence regulator ComR from Streptococcus vestibular...

New page

'''Unreleased structure'''

The entry 6hua is ON HOLD

Authors: Nessler, S., Thuillier, J., Ledesma, L., Hols, P.

Description: the competence regulator ComR from Streptococcus vestibularis in complex with its cognate signaling peptide XIP
[[Category: Unreleased Structures]]
[[Category: Thuillier, J]]
[[Category: Hols, P]]
[[Category: Ledesma, L]]
[[Category: Nessler, S]]