6i89 - Revision history

OCA at 05:43, 13 May 2020

2020-05-13T05:43:28Z

←Older revision		Revision as of 05:43, 13 May 2020
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	<StructureSection load='6i89' size='340' side='right'caption='[[6i89]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6i89' size='340' side='right'caption='[[6i89]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6i89]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I89 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6I89 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6i89]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I89 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6I89 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6i89 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i89 OCA], [http://pdbe.org/6i89 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i89 RCSB], [http://www.ebi.ac.uk/pdbsum/6i89 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i89 ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ardC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6i89 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i89 OCA], [http://pdbe.org/6i89 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i89 RCSB], [http://www.ebi.ac.uk/pdbsum/6i89 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i89 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Plasmids, when transferred by conjugation in natural environments, must overpass restriction-modification systems of the recipient cell. We demonstrate that protein ArdC, encoded by broad host range plasmid R388, was required for conjugation from Escherichia coli to Pseudomonas putida. Expression of ardC was required in the recipient cells, but not in the donor cells. Besides, ardC was not required for conjugation if the hsdRMS system was deleted in P. putida recipient cells. ardC was also required if the hsdRMS system was present in E. coli recipient cells. Thus, ArdC has antirestriction activity against the HsdRMS system and consequently broadens R388 plasmid host range. The crystal structure of ArdC was solved both in the absence and presence of Mn2+. ArdC is composed of a non-specific ssDNA binding N-terminal domain and a C-terminal metalloprotease domain, although the metalloprotease activity was not needed for the antirestriction function. We also observed by RNA-seq that ArdC-dependent conjugation triggered an SOS response in the P. putida recipient cells. Our findings give new insights, and open new questions, into the antirestriction strategies developed by plasmids to counteract bacterial restriction strategies and settle into new hosts.
		+
		+	ArdC, a ssDNA-binding protein with a metalloprotease domain, overpasses the recipient hsdRMS restriction system broadening conjugation host range.,Gonzalez-Montes L, Del Campo I, Garcillan-Barcia MP, de la Cruz F, Moncalian G PLoS Genet. 2020 Apr 29;16(4):e1008750. doi: 10.1371/journal.pgen.1008750., eCollection 2020 Apr. PMID:32348296<ref>PMID:32348296</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6i89" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Gonzalez-Montes, L]]		[[Category: Gonzalez-Montes, L]]

OCA at 09:37, 18 March 2020

2020-03-18T09:37:19Z

←Older revision		Revision as of 09:37, 18 March 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6i89 ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of Antirestriction ArdC protein from R388 plasmid. Metal-free structure.==
-		+	<StructureSection load='6i89' size='340' side='right'caption='[[6i89]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6i89]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I89 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I89 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6i89 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i89 OCA], [http://pdbe.org/6i89 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i89 RCSB], [http://www.ebi.ac.uk/pdbsum/6i89 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i89 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Gonzalez-Montes, L]]
		+	[[Category: Moncalian, G]]
		+	[[Category: Dna binding protein metalloprotease antirestriction]]
		+	[[Category: Metal binding protein]]

OCA at 04:58, 5 December 2018

2018-12-05T04:58:27Z

←Older revision		Revision as of 04:58, 5 December 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6i89 is ON HOLD	+	The entry 6i89 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6i89" [edit=sysop:move=sysop]

2018-12-02T18:44:10Z

Protected "6i89" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:44, 2 December 2018

OCA: New page: '''Unreleased structure''' The entry 6i89 is ON HOLD Authors: Description: Category: Unreleased Structures

2018-12-02T18:44:08Z

New page: '''Unreleased structure''' The entry 6i89 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6i89 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]