6iml - Revision history

OCA at 09:46, 22 November 2023

2023-11-22T09:46:07Z

←Older revision		Revision as of 09:46, 22 November 2023
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	==The crystal structure of AsfvLIG:CT1 complex==		==The crystal structure of AsfvLIG:CT1 complex==
-	<StructureSection load='6iml' size='340' side='right' caption='[[6iml]], [[Resolution\|resolution]] 2.35Å' scene=''>	+	<StructureSection load='6iml' size='340' side='right'caption='[[6iml]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6iml]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IML OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6IML FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6iml]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/African_swine_fever_virus African swine fever virus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IML OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IML FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6iml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iml OCA], [~~http~~://pdbe.org/6iml PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6iml RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6iml PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6iml ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.35Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6iml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iml OCA], [https://pdbe.org/6iml PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6iml RCSB], [https://www.ebi.ac.uk/pdbsum/6iml PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6iml ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/DNLI_ASFB7 DNLI_ASFB7] Very low-fidelity DNA ligase that seals nicks in double-stranded DNA during DNA repair (PubMed:15938630). Together with the viral repair DNA polymerase X, fills the single nucleotide gaps generated by the AP endonuclease (PubMed:15938630). It is not essential for viral replication and recombination (PubMed:15938630). Displays a very low adenylation activity towards DNA with 3'-dideoxy- or 3'-amino-terminated nicks compared to regular nick DNA (PubMed:15938630).<ref>PMID:15938630</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 6iml" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6iml" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[DNA ligase 3D structures\|DNA ligase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chen, Y Q~~]]	+	[[Category: African swine fever virus]]
-	[[Category: ~~Gan, J H~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Dna binding protein~~]]	+	[[Category: Synthetic construct]]
-	[[Category: ~~Ligase-dna complex~~]]	+	[[Category: Chen YQ]]
-	[[Category: ~~The crysatl structure of asfvlig with c:g complex~~]]	+	[[Category: Gan JH]]

OCA at 15:26, 27 February 2019

2019-02-27T15:26:16Z

←Older revision		Revision as of 15:26, 27 February 2019
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-	'''Unreleased structure'''

-	The ~~entry~~ 6iml is ~~ON HOLD until Paper~~ Publication	+	==The crystal structure of AsfvLIG:CT1 complex==
		+	<StructureSection load='6iml' size='340' side='right' caption='[[6iml]], [[Resolution\|resolution]] 2.35Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6iml]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IML OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IML FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6iml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iml OCA], [http://pdbe.org/6iml PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6iml RCSB], [http://www.ebi.ac.uk/pdbsum/6iml PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6iml ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	African swine fever virus (ASFV) is contagious and can cause highly lethal disease in pigs. ASFV DNA ligase (AsfvLIG) is one of the most error-prone ligases identified to date; it catalyzes DNA joining reaction during DNA repair process of ASFV and plays important roles in mutagenesis of the viral genome. Here, we report four AsfvLIG:DNA complex structures and demonstrate that AsfvLIG has a unique N-terminal domain (NTD) that plays critical roles in substrate binding and catalytic complex assembly. In combination with mutagenesis, in vitro binding and catalytic assays, our study reveals that four unique active site residues (Asn153 and Leu211 of the AD domain; Leu402 and Gln403 of the OB domain) are crucial for the catalytic efficiency of AsfvLIG. These unique structural features can serve as potential targets for small molecule design, which could impair genome repair in ASFV and help combat this virus in the future.

-	~~Authors:~~ Chen, Y~~.Q.~~, ~~Gan~~, J.H.	+	Structure of the error-prone DNA ligase of African swine fever virus identifies critical active site residues.,Chen Y, Liu H, Yang C, Gao Y, Yu X, Chen X, Cui R, Zheng L, Li S, Li X, Ma J, Huang Z, Li J, Gan J Nat Commun. 2019 Jan 23;10(1):387. doi: 10.1038/s41467-019-08296-w. PMID:30674878<ref>PMID:30674878</ref>

-	~~Description: The crystal structure~~ of ~~AsfvLIG~~:~~CT1 complex~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Gan, J.H]]	+	<div class="pdbe-citations 6iml" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Chen, Y.Q~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chen, Y Q]]
		+	[[Category: Gan, J H]]
		+	[[Category: Dna binding protein]]
		+	[[Category: Ligase-dna complex]]
		+	[[Category: The crysatl structure of asfvlig with c:g complex]]

OCA at 06:28, 14 November 2018

2018-11-14T06:28:12Z

←Older revision		Revision as of 06:28, 14 November 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6iml is ON HOLD	+	The entry 6iml is ON HOLD until Paper Publication

	Authors: Chen, Y.Q., Gan, J.H.		Authors: Chen, Y.Q., Gan, J.H.

OCA: Protected "6iml" [edit=sysop:move=sysop]

2018-10-31T05:21:51Z

Protected "6iml" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:21, 31 October 2018

OCA: New page: '''Unreleased structure''' The entry 6iml is ON HOLD Authors: Chen, Y.Q., Gan, J.H. Description: The crystal structure of AsfvLIG:CT1 complex Category: Unreleased Structures [[Cate...

2018-10-31T05:21:49Z

New page: '''Unreleased structure''' The entry 6iml is ON HOLD Authors: Chen, Y.Q., Gan, J.H. Description: The crystal structure of AsfvLIG:CT1 complex Category: Unreleased Structures [[Cate...

New page

'''Unreleased structure'''

The entry 6iml is ON HOLD

Authors: Chen, Y.Q., Gan, J.H.

Description: The crystal structure of AsfvLIG:CT1 complex
[[Category: Unreleased Structures]]
[[Category: Gan, J.H]]
[[Category: Chen, Y.Q]]