6isp - Revision history

OCA at 09:52, 22 November 2023

2023-11-22T09:52:35Z

←Older revision		Revision as of 09:52, 22 November 2023
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	<StructureSection load='6isp' size='340' side='right'caption='[[6isp]], [[Resolution\|resolution]] 1.88Å' scene=''>		<StructureSection load='6isp' size='340' side='right'caption='[[6isp]], [[Resolution\|resolution]] 1.88Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6isp]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_34888 Atcc 34888~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ISP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ISP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6isp]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Moesziomyces_antarcticus Moesziomyces antarcticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ISP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ISP FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=CA:CALCIUM+ION'>CA</scene~~>, ~~<scene name='pdbligand=CPQ:N,N-BIS(3-D-GLUCONAMIDOPROPYL)DEOXYCHOLAMIDE'>CPQ</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.88Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http:~~/~~/en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase]~~, ~~with EC number [http~~:~~//www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=~~3~~.1.1.3 3.1.1.3]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CPQ:N,N-BIS(3-D-GLUCONAMIDOPROPYL)DEOXYCHOLAMIDE'>CPQ</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6isp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6isp OCA], [~~http~~://pdbe.org/6isp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6isp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6isp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6isp ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6isp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6isp OCA], [https://pdbe.org/6isp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6isp RCSB], [https://www.ebi.ac.uk/pdbsum/6isp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6isp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/LIPB_PSEA2 LIPB_PSEA2]] Hydrolysis of triglycerides. Is very stereospecific both in hydrolysis and in organic synthesis and has a potentially important application in glucolipid synthesis.	+	[https://www.uniprot.org/uniprot/LIPB_PSEA2 LIPB_PSEA2] Hydrolysis of triglycerides. Is very stereospecific both in hydrolysis and in organic synthesis and has a potentially important application in glucolipid synthesis.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6isp" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6isp" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Lipase 3D Structures\|Lipase 3D Structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 34888]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Triacylglycerol lipase~~]]	+	[[Category: Moesziomyces antarcticus]]
-	[[Category: Cen~~, Y X~~]]	+	[[Category: Cen YX]]
-	[[Category: Wu, Q]]	+	[[Category: Wu Q]]
-	[[Category: Zhou~~, J H]]~~	+	[[Category: Zhou JH]]
-	~~[[Category: Calb]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 06:16, 7 August 2019

2019-08-07T06:16:10Z

←Older revision		Revision as of 06:16, 7 August 2019
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	<StructureSection load='6isp' size='340' side='right'caption='[[6isp]], [[Resolution\|resolution]] 1.88Å' scene=''>		<StructureSection load='6isp' size='340' side='right'caption='[[6isp]], [[Resolution\|resolution]] 1.88Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6isp]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ISP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ISP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6isp]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_34888 Atcc 34888]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ISP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ISP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CPQ:N,N-BIS(3-D-GLUCONAMIDOPROPYL)DEOXYCHOLAMIDE'>CPQ</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CPQ:N,N-BIS(3-D-GLUCONAMIDOPROPYL)DEOXYCHOLAMIDE'>CPQ</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
Line 10:		Line 10:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/LIPB_PSEA2 LIPB_PSEA2]] Hydrolysis of triglycerides. Is very stereospecific both in hydrolysis and in organic synthesis and has a potentially important application in glucolipid synthesis.		[[http://www.uniprot.org/uniprot/LIPB_PSEA2 LIPB_PSEA2]] Hydrolysis of triglycerides. Is very stereospecific both in hydrolysis and in organic synthesis and has a potentially important application in glucolipid synthesis.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Engineering artificial enzymes with high activity and catalytic mechanism different from naturally occurring enzymes is a challenge in protein design. For example, many attempts have been made to obtain active hydrolases by introducing a Ser --> Cys exchange at the respective catalytic triads, but this generally induced a breakdown of activity. We now report that this long-standing dogma no longer pertains, provided additional mutations are introduced by directed evolution. By employing Candida antarctica lipase B (CALB) as the model enzyme with the Ser-His-Asp catalytic triad, a highly active cysteine-lipase having a Cys-His-Asp catalytic triad and additional mutations W104V/A281Y/A282Y/V149G can be evolved, showing a 40-fold higher catalytic efficiency than wild-type CALB in the hydrolysis of 4-nitrophenyl benzoate, and tolerating bulky substrates. Crystal structures, kinetics, MD simulations and QM/MM calculations reveal dynamic features and explain all results, including the preference of a two-step mechanism involving the zwitterionic pair Cys105(-)/His224(+) rather than a concerted process.
		+
		+	Artificial cysteine-lipases with high activity and altered catalytic mechanism created by laboratory evolution.,Cen Y, Singh W, Arkin M, Moody TS, Huang M, Zhou J, Wu Q, Reetz MT Nat Commun. 2019 Jul 19;10(1):3198. doi: 10.1038/s41467-019-11155-3. PMID:31324776<ref>PMID:31324776</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6isp" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 34888]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]

OCA at 06:11, 24 July 2019

2019-07-24T06:11:58Z

←Older revision		Revision as of 06:11, 24 July 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6isp is ~~ON HOLD until Paper Publication~~	+	==structure of Candida antarctica Lipase B mutant==
-		+	<StructureSection load='6isp' size='340' side='right'caption='[[6isp]], [[Resolution\|resolution]] 1.88Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6isp]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ISP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ISP FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CPQ:N,N-BIS(3-D-GLUCONAMIDOPROPYL)DEOXYCHOLAMIDE'>CPQ</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6isp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6isp OCA], [http://pdbe.org/6isp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6isp RCSB], [http://www.ebi.ac.uk/pdbsum/6isp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6isp ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LIPB_PSEA2 LIPB_PSEA2]] Hydrolysis of triglycerides. Is very stereospecific both in hydrolysis and in organic synthesis and has a potentially important application in glucolipid synthesis.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Triacylglycerol lipase]]
		+	[[Category: Cen, Y X]]
		+	[[Category: Wu, Q]]
		+	[[Category: Zhou, J H]]
		+	[[Category: Calb]]
		+	[[Category: Hydrolase]]

OCA at 04:22, 3 April 2019

2019-04-03T04:22:57Z

←Older revision		Revision as of 04:22, 3 April 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6isp is ON HOLD	+	The entry 6isp is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 04:49, 27 March 2019

2019-03-27T04:49:13Z

←Older revision		Revision as of 04:49, 27 March 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6isp is ON HOLD ~~until Paper Publication~~	+	The entry 6isp is ON HOLD

	Authors:		Authors:

OCA at 05:09, 12 December 2018

2018-12-12T05:09:30Z

←Older revision		Revision as of 05:09, 12 December 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6isp is ON HOLD	+	The entry 6isp is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6isp" [edit=sysop:move=sysop]

2018-12-02T18:56:48Z

Protected "6isp" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:56, 2 December 2018

OCA: New page: '''Unreleased structure''' The entry 6isp is ON HOLD Authors: Description: Category: Unreleased Structures

2018-12-02T18:56:47Z

New page: '''Unreleased structure''' The entry 6isp is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6isp is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]