OCA at 09:58, 22 November 2023

2023-11-22T09:58:55Z

←Older revision		Revision as of 09:58, 22 November 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6j31 is ~~ON HOLD until Paper~~ Publication	+	==Crystal Structure Analysis of the Glycotransferase of kitacinnamycin==
		+	<StructureSection load='6j31' size='340' side='right'caption='[[6j31]], [[Resolution\|resolution]] 2.24Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6j31]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Kitasatospora Kitasatospora] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J31 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J31 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.244Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BG9:(2E,2E)-3,3-(1,2-phenylene)di(prop-2-enoic+acid)'>BG9</scene>, <scene name='pdbligand=DBB:D-ALPHA-AMINOBUTYRIC+ACID'>DBB</scene>, <scene name='pdbligand=DLE:D-LEUCINE'>DLE</scene>, <scene name='pdbligand=DSG:D-ASPARAGINE'>DSG</scene>, <scene name='pdbligand=DVA:D-VALINE'>DVA</scene>, <scene name='pdbligand=MEA:N-METHYLPHENYLALANINE'>MEA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j31 OCA], [https://pdbe.org/6j31 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j31 RCSB], [https://www.ebi.ac.uk/pdbsum/6j31 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j31 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A514S208_9ACTN A0A514S208_9ACTN]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cinnamoyl-containing nonribosomal peptides (CCNPs) are a small group of secondary metabolites with potent biological activities produced by actinobacteria. Two remarkable features in the biosynthesis of CCNPs include the nonribosomal peptide synthases (NRPSs) for assembly of the depsipeptide backbone and the type II polyketide synthases (PKSs) for N-terminal cinnamoyl moiety construction. Here, we present a genome mining approach targeting both NRPS and type II PKS for discovery of new CCNPs, which led to the identification of 51 putative CCNP gene clusters from public bacterial genome databases. After strain prioritization, a novel class of CCNP-type glycopeptides named kitacinnamycins, one of which showing potent activation ability towards the stimulator of interferon genes (STING) protein, was identified. Bioinformatic, genetic and biochemical analysis revealed the use of the NRPS assembly line to form the macrocyclic peptide backbone, followed by a P450 monooxygenase to generate terminal oxidized groups. A glycosyltransferase with relatively broad substrate specificity transfers sugars to the newly generated OH/COOH group. The protein crystallographic study further provided structural insights into this glycosylation. Our results not only demonstrated the feasibility of genome mining and strain prioritization for the discovery of new bioactive natural products but also disclosed the biosynthetic pathway for kitacinnamycins.

-	~~Authors: Shi~~, J., Liu~~, C.L.~~, Zhang, B., Guo~~, W.J.~~, Zhu, J~~.P.~~, Xu, ~~X., Xu, Q.~~, Jiao~~, R.H.~~, Tan, R.X., ~~Ge, H.M~~.	+	Genome mining and biosynthesis of kitacinnamycins as a STING activator.,Shi J, Liu CL, Zhang B, Guo WJ, Zhu J, Chang CY, Zhao EJ, Jiao RH, Tan RX, Ge HM Chem Sci. 2019 Apr 2;10(18):4839-4846. doi: 10.1039/c9sc00815b. eCollection 2019 , May 14. PMID:31160959<ref>PMID:31160959</ref>

-	~~Description: Crystal Structure Analysis~~ of the ~~Glycotransferase~~ of ~~kitacinnamycin~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Zhang, B~~]]	+	<div class="pdbe-citations 6j31" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Jiao, R.H~~]]	+
-	[[Category: ~~Liu, C.L~~]]	+	==See Also==
-	[[Category: ~~Zhu, J.P~~]]	+	*[[Glycosyltransferase 3D structures\|Glycosyltransferase 3D structures]]
-	[[Category: ~~Xu, Q~~]]	+	== References ==
-	[[Category: ~~Ge, H.M~~]]	+	<references/>
-	[[Category: ~~Tan, R.X~~]]	+	__TOC__
-	[[Category: ~~Guo, W.J~~]]	+	</StructureSection>
-	[[Category: ~~Shi, J~~]]	+	[[Category: Kitasatospora]]
-	[[Category: Xu, X]]	+	[[Category: Large Structures]]
		+	[[Category: Synthetic construct]]
		+	[[Category: Ge HM]]
		+	[[Category: Guo WJ]]
		+	[[Category: Jiao RH]]
		+	[[Category: Liu CL]]
		+	[[Category: Shi J]]
		+	[[Category: Tan RX]]
		+	[[Category: Xu Q]]
		+	[[Category: Xu X]]
		+	[[Category: Zhang B]]
		+	[[Category: Zhu JP]]

OCA at 07:11, 13 February 2019

2019-02-13T07:11:39Z

←Older revision		Revision as of 07:11, 13 February 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6j31 is ON HOLD	+	The entry 6j31 is ON HOLD until Paper Publication

	Authors: Shi, J., Liu, C.L., Zhang, B., Guo, W.J., Zhu, J.P., Xu, X., Xu, Q., Jiao, R.H., Tan, R.X., Ge, H.M.		Authors: Shi, J., Liu, C.L., Zhang, B., Guo, W.J., Zhu, J.P., Xu, X., Xu, Q., Jiao, R.H., Tan, R.X., Ge, H.M.
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	[[Category: Zhang, B]]		[[Category: Zhang, B]]
	[[Category: Jiao, R.H]]		[[Category: Jiao, R.H]]
-	[[Category: Tan, R.X]]
	[[Category: Liu, C.L]]		[[Category: Liu, C.L]]
	[[Category: Zhu, J.P]]		[[Category: Zhu, J.P]]
		+	[[Category: Xu, Q]]
		+	[[Category: Ge, H.M]]
		+	[[Category: Tan, R.X]]
	[[Category: Guo, W.J]]		[[Category: Guo, W.J]]
	[[Category: Shi, J]]		[[Category: Shi, J]]
-	[[Category: Xu, Q]]
	[[Category: Xu, X]]		[[Category: Xu, X]]
-	[[Category: Ge, H.M]]

OCA: Protected "6j31" [edit=sysop:move=sysop]

2019-01-09T04:58:55Z

Protected "6j31" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:58, 9 January 2019

OCA: New page: '''Unreleased structure''' The entry 6j31 is ON HOLD Authors: Shi, J., Liu, C.L., Zhang, B., Guo, W.J., Zhu, J.P., Xu, X., Xu, Q., Jiao, R.H., Tan, R.X., Ge, H.M. Description: Crystal ...

2019-01-09T04:58:53Z

New page: '''Unreleased structure''' The entry 6j31 is ON HOLD Authors: Shi, J., Liu, C.L., Zhang, B., Guo, W.J., Zhu, J.P., Xu, X., Xu, Q., Jiao, R.H., Tan, R.X., Ge, H.M. Description: Crystal ...

New page

'''Unreleased structure'''

The entry 6j31 is ON HOLD

Authors: Shi, J., Liu, C.L., Zhang, B., Guo, W.J., Zhu, J.P., Xu, X., Xu, Q., Jiao, R.H., Tan, R.X., Ge, H.M.

Description: Crystal Structure Analysis of the Glycotransferase of kitacinnamycin
[[Category: Unreleased Structures]]
[[Category: Zhang, B]]
[[Category: Jiao, R.H]]
[[Category: Tan, R.X]]
[[Category: Liu, C.L]]
[[Category: Zhu, J.P]]
[[Category: Guo, W.J]]
[[Category: Shi, J]]
[[Category: Xu, Q]]
[[Category: Xu, X]]
[[Category: Ge, H.M]]

6j31 - Revision history

OCA at 09:58, 22 November 2023

OCA at 07:11, 13 February 2019

OCA: Protected "6j31" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6j31 is ON HOLD Authors: Shi, J., Liu, C.L., Zhang, B., Guo, W.J., Zhu, J.P., Xu, X., Xu, Q., Jiao, R.H., Tan, R.X., Ge, H.M. Description: Crystal ...