6j8w - Revision history

OCA at 10:03, 22 November 2023

2023-11-22T10:03:40Z

←Older revision		Revision as of 10:03, 22 November 2023
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	<StructureSection load='6j8w' size='340' side='right'caption='[[6j8w]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='6j8w' size='340' side='right'caption='[[6j8w]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6j8w]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J8W OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6J8W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6j8w]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2] and [https://en.wikipedia.org/wiki/Streptomyces_viridosporus Streptomyces viridosporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J8W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J8W FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C0X:(2S)-3-dimethoxyphosphoryloxy-2-[(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trienoxy]propanoic+acid'>C0X</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.35Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5gwv\|5gwv]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C0X:(2S)-3-dimethoxyphosphoryloxy-2-[(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trienoxy]propanoic+acid'>C0X</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6j8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j8w OCA], [~~http~~://pdbe.org/6j8w PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6j8w RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6j8w PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6j8w ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j8w OCA], [https://pdbe.org/6j8w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j8w RCSB], [https://www.ebi.ac.uk/pdbsum/6j8w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j8w ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A010_STRVD A0A010_STRVD] [https://www.uniprot.org/uniprot/DN7D_SACS2 DN7D_SACS2] Can constrain negative DNA supercoils. May be involved in maintaining the integrity of the genome at high temperature (By similarity). Stimulates the Holliday junction cleavage activity of Hjc (PubMed:11709558).[UniProtKB:P61990]<ref>PMID:11709558</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Chen, C C~~]]	+	[[Category: Saccharolobus solfataricus P2]]
-	[[Category: ~~Guo, R T~~]]	+	[[Category: Streptomyces viridosporus]]
-	[[Category: ~~Ko, T P~~]]	+	[[Category: Chen CC]]
-	[[Category: ~~Zhang, L L~~]]	+	[[Category: Guo RT]]
-	[[Category: ~~Alpha~~]]	+	[[Category: Ko TP]]
-	[[Category: ~~Antibiotic]]~~	+	[[Category: Zhang LL]]
-	~~[[Category: Biosynthesis]]~~	+
-	~~[[Category: Moenomycin]]~~	+
-	~~[[Category: Prenyl transferase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 05:59, 17 April 2019

2019-04-17T05:59:55Z

←Older revision		Revision as of 05:59, 17 April 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6j8w is ~~ON HOLD~~	+	==Structure of MOEN5-SSO7D fusion protein in complex with lig 1==
		+	<StructureSection load='6j8w' size='340' side='right'caption='[[6j8w]], [[Resolution\|resolution]] 2.35Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6j8w]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J8W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6J8W FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C0X:(2S)-3-dimethoxyphosphoryloxy-2-[(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trienoxy]propanoic+acid'>C0X</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5gwv\|5gwv]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6j8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j8w OCA], [http://pdbe.org/6j8w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j8w RCSB], [http://www.ebi.ac.uk/pdbsum/6j8w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j8w ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The antibiotic moenomycin A is a phosphoglycerate derivative with a C25-moenocinyl chain and a branched oligosaccharide. Formation of the C25-chain is catalyzed by the enzyme MoeN5 with geranyl pyrophosphate (GPP) and the sugar-linked 2-Z,E-farnesyl-3-phosphoglycerate (FPG) as its substrates. Previous complex crystal structures with GPP and long-chain alkyl glycosides suggested that GPP binds to the S1 site in a similar way as in most other alpha-helical prenyltransferases (PTs), and FPG is likely to assume a bent conformation in the S2 site. However, two FPG derivatives synthesized in the current study were found in the S1 site rather than S2 in their complex crystal structures with MoeN5. Apparently S1 is the preferred site for prenyl-containing ligand, and S2 binding may proceed only after S1 is occupied. Thus, like most trans-type PTs, MoeN5 may employ a sequential ionization-condensation-elimination mechanism that involves a carbocation intermediate.

-	~~Authors: Ko, T.P~~., Zhang, L~~.L.~~, ~~Chen~~, ~~C.C.~~, Guo, R.T.	+	Complex structures of MoeN5 with substrate analogues suggest sequential catalytic mechanism.,Zhang L, Ko TP, Malwal SR, Liu W, Zhou S, Yu X, Oldfield E, Guo RT, Chen CC Biochem Biophys Res Commun. 2019 Apr 16;511(4):800-805. doi:, 10.1016/j.bbrc.2019.02.131. Epub 2019 Mar 2. PMID:30837154<ref>PMID:30837154</ref>

-	~~Description: Structure~~ of ~~MOEN5~~-~~SSO7D fusion protein in complex with lig 1~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Chen, C.C]]	+	<div class="pdbe-citations 6j8w" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Zhang~~, ~~L.L~~]]	+	== References ==
-	[[Category: Ko, T.P]]	+	<references/>
-	[[Category: ~~Guo~~, ~~R.T~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Chen, C C]]
		+	[[Category: Guo, R T]]
		+	[[Category: Ko, T P]]
		+	[[Category: Zhang, L L]]
		+	[[Category: Alpha]]
		+	[[Category: Antibiotic]]
		+	[[Category: Biosynthesis]]
		+	[[Category: Moenomycin]]
		+	[[Category: Prenyl transferase]]
		+	[[Category: Transferase]]

OCA at 05:23, 6 March 2019

2019-03-06T05:23:37Z

←Older revision		Revision as of 05:23, 6 March 2019
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	Authors: Ko, T.P., Zhang, L.L., Chen, C.C., Guo, R.T.		Authors: Ko, T.P., Zhang, L.L., Chen, C.C., Guo, R.T.

-	Description: ~~STRUCTURE OF~~ MOEN5-SSO7D ~~FUSION PROTEIN IN COMPLEX WITH~~ lig 1	+	Description: Structure of MOEN5-SSO7D fusion protein in complex with lig 1
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Chen, C.C]]		[[Category: Chen, C.C]]

OCA: Protected "6j8w" [edit=sysop:move=sysop]

2019-01-30T06:15:34Z

Protected "6j8w" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:15, 30 January 2019

OCA: New page: '''Unreleased structure''' The entry 6j8w is ON HOLD Authors: Ko, T.P., Zhang, L.L., Chen, C.C., Guo, R.T. Description: STRUCTURE OF MOEN5-SSO7D FUSION PROTEIN IN COMPLEX WITH lig 1 [[...

2019-01-30T06:15:33Z

New page: '''Unreleased structure''' The entry 6j8w is ON HOLD Authors: Ko, T.P., Zhang, L.L., Chen, C.C., Guo, R.T. Description: STRUCTURE OF MOEN5-SSO7D FUSION PROTEIN IN COMPLEX WITH lig 1 [[...

New page

'''Unreleased structure'''

The entry 6j8w is ON HOLD

Authors: Ko, T.P., Zhang, L.L., Chen, C.C., Guo, R.T.

Description: STRUCTURE OF MOEN5-SSO7D FUSION PROTEIN IN COMPLEX WITH lig 1
[[Category: Unreleased Structures]]
[[Category: Chen, C.C]]
[[Category: Zhang, L.L]]
[[Category: Ko, T.P]]
[[Category: Guo, R.T]]