6jca - Revision history

OCA at 10:06, 22 November 2023

2023-11-22T10:06:25Z

←Older revision		Revision as of 10:06, 22 November 2023
Line 3:		Line 3:
	<StructureSection load='6jca' size='340' side='right'caption='[[6jca]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='6jca' size='340' side='right'caption='[[6jca]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6jca]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Actinoalloteichus_sp.~~_wh1~~-2216-6 Actinoalloteichus sp. ~~wh1~~-2216-6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JCA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6JCA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6jca]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Actinoalloteichus_sp._WH1-2216-6 Actinoalloteichus sp. WH1-2216-6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JCA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JCA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6jca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jca OCA], [~~http~~://pdbe.org/6jca PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6jca RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6jca PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6jca ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jca OCA], [https://pdbe.org/6jca PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jca RCSB], [https://www.ebi.ac.uk/pdbsum/6jca PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jca ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/H8Y6N2_9PSEU H8Y6N2_9PSEU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Actinoalloteichus sp. ~~wh1~~-2216-6]]	+	[[Category: Actinoalloteichus sp. WH1-2216-6]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Liu, J]]	+	[[Category: Liu J]]
-	[[Category: Xu, J]]	+	[[Category: Xu J]]
-	~~[[Category: Aminotransferase]]~~	+
-	~~[[Category: Crmg]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 10:01, 16 September 2020

2020-09-16T10:01:47Z

←Older revision		Revision as of 10:01, 16 September 2020
Line 3:		Line 3:
	<StructureSection load='6jca' size='340' side='right'caption='[[6jca]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='6jca' size='340' side='right'caption='[[6jca]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6jca]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JCA OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6JCA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6jca]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Actinoalloteichus_sp._wh1-2216-6 Actinoalloteichus sp. wh1-2216-6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JCA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6JCA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6jca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jca OCA], [http://pdbe.org/6jca PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jca RCSB], [http://www.ebi.ac.uk/pdbsum/6jca PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jca ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6jca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jca OCA], [http://pdbe.org/6jca PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jca RCSB], [http://www.ebi.ac.uk/pdbsum/6jca PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jca ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Amine compounds biosynthesis using omega-transaminases has received considerable attention in the pharmaceutical industry. However, the application of omega-transaminases was hampered by the fundamental challenge of severe by-product inhibition. Here, we report that omega-transaminase CrmG from Actinoalloteichus cyanogriseus WH1-2216-6 is insensitive to inhibition from by-product alpha-ketoglutarate or pyruvate. Combined with structural and QM/MM studies, we establish the detailed catalytic mechanism for CrmG. Our structural and biochemical studies reveal that the roof of the active site in PMP-bound CrmG is flexible, which will facilitate the PMP or by-product to dissociate from PMP-bound CrmG. Our results also show that amino acceptor caerulomycin M (CRM M), but not alpha-ketoglutarate or pyruvate, can form strong interactions with the roof of the active site in PMP-bound CrmG. Based on our results, we propose that the flexible roof of the active site in PMP-bound CrmG may facilitate CrmG to overcome inhibition from the by-product.
		+
		+	Structural studies reveal flexible roof of active site responsible for omega-transaminase CrmG overcoming by-product inhibition.,Xu J, Tang X, Zhu Y, Yu Z, Su K, Zhang Y, Dong Y, Zhu W, Zhang C, Wu R, Liu J Commun Biol. 2020 Aug 19;3(1):455. doi: 10.1038/s42003-020-01184-w. PMID:32814814<ref>PMID:32814814</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6jca" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Actinoalloteichus sp. wh1-2216-6]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Liu, J]]		[[Category: Liu, J]]

OCA at 07:57, 5 February 2020

2020-02-05T07:57:38Z

←Older revision		Revision as of 07:57, 5 February 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6jca is ON HOLD until Paper Publication~~	+	==Crystal structure of aminotransferase CrmG from Actinoalloteichus sp. WH1-2216-6 in I222 space group==
-		+	<StructureSection load='6jca' size='340' side='right'caption='[[6jca]], [[Resolution\|resolution]] 2.10Å' scene=''>
-	~~Authors: Xu, J., Liu, J.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6jca]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JCA FirstGlance]. <br>
-	~~Description:~~ Crystal structure of aminotransferase CrmG from Actinoalloteichus sp. WH1-2216-6 in I222 space group	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jca OCA], [http://pdbe.org/6jca PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jca RCSB], [http://www.ebi.ac.uk/pdbsum/6jca PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jca ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Liu, J]]
	[[Category: Xu, J]]		[[Category: Xu, J]]
-	[[Category: ~~Liu, J~~]]	+	[[Category: Aminotransferase]]
		+	[[Category: Crmg]]
		+	[[Category: Transferase]]

OCA: Protected "6jca" [edit=sysop:move=sysop]

2019-02-13T07:21:23Z

Protected "6jca" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:21, 13 February 2019

OCA: New page: '''Unreleased structure''' The entry 6jca is ON HOLD until Paper Publication Authors: Xu, J., Liu, J. Description: Crystal structure of aminotransferase CrmG from Actinoalloteichus sp....

2019-02-13T07:21:21Z

New page: '''Unreleased structure''' The entry 6jca is ON HOLD until Paper Publication Authors: Xu, J., Liu, J. Description: Crystal structure of aminotransferase CrmG from Actinoalloteichus sp....

New page

'''Unreleased structure'''

The entry 6jca is ON HOLD until Paper Publication

Authors: Xu, J., Liu, J.

Description: Crystal structure of aminotransferase CrmG from Actinoalloteichus sp. WH1-2216-6 in I222 space group
[[Category: Unreleased Structures]]
[[Category: Xu, J]]
[[Category: Liu, J]]