OCA at 17:28, 20 November 2019

2019-11-20T17:28:47Z

←Older revision		Revision as of 17:28, 20 November 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6jhk is ~~ON HOLD until Paper~~ Publication	+	==Crystal Structure of Bacillus subtilis RsbS==
		+	<StructureSection load='6jhk' size='340' side='right'caption='[[6jhk]], [[Resolution\|resolution]] 3.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6jhk]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JHK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JHK FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">B4122_0858, B4417_4413, CJ481_02205, ETA10_02695, ETK61_02665, ETK71_02600 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jhk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jhk OCA], [http://pdbe.org/6jhk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jhk RCSB], [http://www.ebi.ac.uk/pdbsum/6jhk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jhk ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The stressosome transduces environmental stress signals to SigB to upregulate SigB-dependent transcription, which is required for bacterial viability. The stressosome core is composed of RsbS and at least one of the RsbR paralogs. A previous cryo-electron microscopy (cryo-EM) structure of the RsbRA-RsbS complex determined under a D2 symmetry restraint showed that the stressosome core forms a pseudo-icosahedron consisting of 60 STAS domains of RsbRA and RsbS. However, it is still unclear how RsbS and one of the RsbR paralogs assemble into the stressosome. Here, an assembly model of the stressosome is presented based on the crystal structure of the RsbS icosahedron and cryo-EM structures of the RsbRA-RsbS complex determined under diverse symmetry restraints (nonsymmetric C1, dihedral D2 and icosahedral I envelopes). 60 monomers of the crystal structure of RsbS fitted well into the I-restrained cryo-EM structure determined at 4.1 A resolution, even though the STAS domains in the I envelope were averaged. This indicates that RsbS and RsbRA share a highly conserved STAS fold. 22 protrusions observed in the C1 envelope, corresponding to dimers of the RsbRA N-domain, allowed the STAS domains of RsbRA and RsbS to be distinguished in the stressosome core. Based on these, the model of the stressosome core was reconstructed. The mutation of RsbRA residues at the binding interface in the model (R189A/Q191A) significantly reduced the interaction between RsbRA and RsbS. These results suggest that nonconserved residues in the conserved STAS folds between RsbS and RsbR paralogs determine stressosome assembly.

-	~~Authors: Kwon~~, E., Pathak, D., ~~Dahal~~, P., ~~Kim~~, D.Y.	+	Structural insights into stressosome assembly.,Kwon E, Pathak D, Kim HU, Dahal P, Ha SC, Lee SS, Jeong H, Jeoung D, Chang HW, Jung HS, Kim DY IUCrJ. 2019 Aug 21;6(Pt 5):938-947. doi: 10.1107/S205225251900945X. eCollection, 2019 Sep 1. PMID:31576226<ref>PMID:31576226</ref>

-	~~Description: Crystal Structure~~ of ~~Bacillus~~ subtilis ~~RsbS~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6jhk" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Vibrio subtilis ehrenberg 1835]]
		+	[[Category: Large Structures]]
	[[Category: Dahal, P]]		[[Category: Dahal, P]]
-	~~[[Category: Pathak, D]]~~	+	[[Category: Kim, D Y]]
-	[[Category: Kim, D.Y]]	+
	[[Category: Kwon, E]]		[[Category: Kwon, E]]
		+	[[Category: Pathak, D]]
		+	[[Category: Icosahedron]]
		+	[[Category: Protein binding]]
		+	[[Category: Rsb]]
		+	[[Category: Stas domain]]
		+	[[Category: Stressosome]]

OCA at 04:14, 29 May 2019

2019-05-29T04:14:19Z

←Older revision		Revision as of 04:14, 29 May 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6jhk is ON HOLD	+	The entry 6jhk is ON HOLD until Paper Publication

	Authors: Kwon, E., Pathak, D., Dahal, P., Kim, D.Y.		Authors: Kwon, E., Pathak, D., Dahal, P., Kim, D.Y.

OCA: Protected "6jhk" [edit=sysop:move=sysop]

2019-02-27T13:36:41Z

Protected "6jhk" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:36, 27 February 2019

OCA: New page: '''Unreleased structure''' The entry 6jhk is ON HOLD Authors: Kwon, E., Pathak, D., Dahal, P., Kim, D.Y. Description: Crystal Structure of Bacillus subtilis RsbS [[Category: Unreleased...

2019-02-27T13:36:40Z

New page: '''Unreleased structure''' The entry 6jhk is ON HOLD Authors: Kwon, E., Pathak, D., Dahal, P., Kim, D.Y. Description: Crystal Structure of Bacillus subtilis RsbS [[Category: Unreleased...

New page

'''Unreleased structure'''

The entry 6jhk is ON HOLD

Authors: Kwon, E., Pathak, D., Dahal, P., Kim, D.Y.

Description: Crystal Structure of Bacillus subtilis RsbS
[[Category: Unreleased Structures]]
[[Category: Dahal, P]]
[[Category: Pathak, D]]
[[Category: Kim, D.Y]]
[[Category: Kwon, E]]

6jhk - Revision history

OCA at 17:28, 20 November 2019

OCA at 04:14, 29 May 2019

OCA: Protected "6jhk" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6jhk is ON HOLD Authors: Kwon, E., Pathak, D., Dahal, P., Kim, D.Y. Description: Crystal Structure of Bacillus subtilis RsbS [[Category: Unreleased...