6k31 - Revision history

OCA at 08:44, 27 November 2019

2019-11-27T08:44:20Z

←Older revision		Revision as of 08:44, 27 November 2019
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	<StructureSection load='6k31' size='340' side='right'caption='[[6k31]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='6k31' size='340' side='right'caption='[[6k31]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6k31]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K31 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6K31 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6k31]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinobacterium_israeli"_(kruse_1896)_sampietro_1908 "actinobacterium israeli" (kruse 1896) sampietro 1908]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K31 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6K31 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6k31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k31 OCA], [http://pdbe.org/6k31 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k31 RCSB], [http://www.ebi.ac.uk/pdbsum/6k31 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k31 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6k31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k31 OCA], [http://pdbe.org/6k31 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k31 RCSB], [http://www.ebi.ac.uk/pdbsum/6k31 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k31 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Inorganic pyrophosphate (PPi) consists of two phosphate molecules and can act as an energy and phosphate donor in cellular reactions similar to ATP. Several kinases use PPi as a substrate and these kinases have recently been suggested to have evolved from ATP-dependent functional homologs, which have significant amino acid sequence similarity to PPi-utilizing enzymes. In contrast, phosphoenolpyruvate carboxykinase (PEPCK) can be divided into three types according to the phosphate donor (ATP, GTP, or PPi), and the amino acid sequence similarity of these PEPCKs is too low to confirm that they share a common ancestor. Here, we solved the crystal structure of a PPi-PEPCK homolog from the bacterium Actinomyces israelii (AiPEPCK) at 2.6 A resolution and compared it with previously reported structures from ATP- and GTP-specific PEPCKs to assess the degrees of similarities and divergences among these PEPCKs and to determine the structural basis for the PPi specificity of PPi-type PEPCKs. These comparisons revealed that the amino acid residues directly contributing to substrate recognition, except for those that recognize purine moieties, are conserved. Furthermore, the order of secondary structure elements between PPi-, ATP- and GTP-specific PEPCKs was strictly conserved. The structure-based comparisons of the three PEPCK types provide key insights into the structural basis for PPi specificity and suggest that all of these PEPCKs are derived from a common ancestor.
		+
		+	Structural comparisons of phosphoenolpyruvate carboxykinases reveal the evolutionary trajectories of these phosphodiester energy-conversion enzymes.,Chiba Y, Miyakawa T, Shimane Y, Takai K, Tanokura M, Nozaki T J Biol Chem. 2019 Oct 28. pii: RA119.010920. doi: 10.1074/jbc.RA119.010920. PMID:31662435<ref>PMID:31662435</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6k31" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:40, 6 November 2019

2019-11-06T06:40:33Z

←Older revision		Revision as of 06:40, 6 November 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6k31 is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of pyrophosphate-dependent phosphoenolpyruvate carboxykinase (PPi-PEPCK)==
-		+	<StructureSection load='6k31' size='340' side='right'caption='[[6k31]], [[Resolution\|resolution]] 2.60Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6k31]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K31 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6K31 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoenolpyruvate_carboxykinase_(diphosphate) Phosphoenolpyruvate carboxykinase (diphosphate)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.38 4.1.1.38] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6k31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k31 OCA], [http://pdbe.org/6k31 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k31 RCSB], [http://www.ebi.ac.uk/pdbsum/6k31 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k31 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Chiba, Y]]
		+	[[Category: Miyakawa, T]]
		+	[[Category: Tanokura, M]]
		+	[[Category: Lyase]]
		+	[[Category: Phosphoenolpyruvate carboxykinase]]

OCA at 04:24, 29 May 2019

2019-05-29T04:24:41Z

←Older revision		Revision as of 04:24, 29 May 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6k31 is ON HOLD	+	The entry 6k31 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6k31" [edit=sysop:move=sysop]

2019-05-22T03:56:58Z

Protected "6k31" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:56, 22 May 2019

OCA: New page: '''Unreleased structure''' The entry 6k31 is ON HOLD Authors: Description: Category: Unreleased Structures

2019-05-22T03:56:56Z

New page: '''Unreleased structure''' The entry 6k31 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6k31 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]