6k7k - Revision history

OCA at 02:20, 11 April 2020

2020-04-11T02:20:57Z

←Older revision		Revision as of 02:20, 11 April 2020
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	<SX load='6k7k' size='340' side='right' viewer='molstar' caption='[[6k7k]], [[Resolution\|resolution]] 3.04Å' scene=''>		<SX load='6k7k' size='340' side='right' viewer='molstar' caption='[[6k7k]], [[Resolution\|resolution]] 3.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6k7k]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K7K OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6K7K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6k7k]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K7K OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6K7K FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CDC50A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CDC50A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/P-type_phospholipid_transporter P-type phospholipid transporter], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=7.6.2.1 7.6.2.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/P-type_phospholipid_transporter P-type phospholipid transporter], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=7.6.2.1 7.6.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6k7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k7k OCA], [http://pdbe.org/6k7k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k7k RCSB], [http://www.ebi.ac.uk/pdbsum/6k7k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k7k ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6k7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k7k OCA], [http://pdbe.org/6k7k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k7k RCSB], [http://www.ebi.ac.uk/pdbsum/6k7k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k7k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 22:23, 6 March 2020

2020-03-06T22:23:06Z

←Older revision		Revision as of 22:23, 6 March 2020
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	==Cryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E1-ADP-Pi state)==		==Cryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E1-ADP-Pi state)==
-	<~~StructureSection~~ load='6k7k' size='340' side='right'caption='[[6k7k]], [[Resolution\|resolution]] 3.04Å' scene=''>	+	<SX load='6k7k' size='340' side='right' viewer='molstar' caption='[[6k7k]], [[Resolution\|resolution]] 3.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6k7k]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K7K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6K7K FirstGlance]. <br>		<table><tr><td colspan='2'>[[6k7k]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K7K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6K7K FirstGlance]. <br>
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	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Human]]		[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 08:19, 6 November 2019

2019-11-06T08:19:02Z

←Older revision		Revision as of 08:19, 6 November 2019
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	<StructureSection load='6k7k' size='340' side='right'caption='[[6k7k]], [[Resolution\|resolution]] 3.04Å' scene=''>		<StructureSection load='6k7k' size='340' side='right'caption='[[6k7k]], [[Resolution\|resolution]] 3.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6k7k]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K7K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6K7K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6k7k]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K7K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6K7K FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CDC50A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/P-type_phospholipid_transporter P-type phospholipid transporter], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=7.6.2.1 7.6.2.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/P-type_phospholipid_transporter P-type phospholipid transporter], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=7.6.2.1 7.6.2.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6k7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k7k OCA], [http://pdbe.org/6k7k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k7k RCSB], [http://www.ebi.ac.uk/pdbsum/6k7k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k7k ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6k7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k7k OCA], [http://pdbe.org/6k7k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k7k RCSB], [http://www.ebi.ac.uk/pdbsum/6k7k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k7k ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: P-type phospholipid transporter]]		[[Category: P-type phospholipid transporter]]

OCA at 15:29, 28 August 2019

2019-08-28T15:29:53Z

←Older revision		Revision as of 15:29, 28 August 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6k7k is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Cryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E1-ADP-Pi state)==
		+	<StructureSection load='6k7k' size='340' side='right'caption='[[6k7k]], [[Resolution\|resolution]] 3.04Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6k7k]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K7K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6K7K FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/P-type_phospholipid_transporter P-type phospholipid transporter], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=7.6.2.1 7.6.2.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6k7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k7k OCA], [http://pdbe.org/6k7k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k7k RCSB], [http://www.ebi.ac.uk/pdbsum/6k7k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k7k ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CC50A_HUMAN CC50A_HUMAN]] Accessory component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. The beta subunit may assist in binding of the phospholipid substrate. Required for the proper folding, assembly and ER to Golgi exit of the ATP8A2:TMEM30A flippase complex. ATP8A2:TMEM30A may be involved in regulation of neurite outgrowth, and, reconstituted to liposomes, predomiminantly transports phosphatidylserine (PS) and to a lesser extent phosphatidylethanolamine (PE). The ATP8A1:TMEM30A flippase complex seems to play a role in regulation of cell migration probably involving flippase-mediated translocation of phosphatidylethanolamine (PE) at the plasma membrane. Required for the formation of the ATP8A2, ATP8B1 and ATP8B2 P-type ATPAse intermediate phosphoenzymes. Involved in uptake of platelet-activating factor (PAF), synthetic drug alkylphospholipid edelfosine, and, probably in association with ATP8B1, of perifosine. Also mediate the export of alpha subunits ATP8A1, ATP8B1, ATP8B2, ATP8B4, ATP10A, ATP10B, ATP10D, ATP11A, ATP11B and ATP11C from the ER to other membrane localizations.<ref>PMID:20510206</ref> <ref>PMID:20947505</ref> <ref>PMID:20961850</ref> <ref>PMID:21289302</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In eukaryotic membranes, P4-ATPases mediate the translocation of phospholipids from the outer to inner leaflet and maintain lipid asymmetry, which is critical for membrane trafficking and signaling pathways. Here we report the cryo-EM structures of six distinct intermediates of the human ATP8A1-CDC50a hetero-complex, at 2.6-3.3 A resolutions, elucidating lipid translocation cycle of this P4-ATPase. ATP-dependent phosphorylation induces a large rotational movement of the actuator domain around the phosphorylation site in the phosphorylation domain, accompanied by lateral shifts of the first and second transmembrane helices, thereby allowing phosphatidylserine binding. The phospholipid head group passes through the hydrophilic cleft, while the acyl chain is exposed toward the lipid environment. These findings advance our understanding of the flippase mechanism and the disease-associated mutants of P4-ATPases.

-	~~Authors~~:	+	Cryo-EM structures capture the transport cycle of the P4-ATPase flippase.,Hiraizumi M, Yamashita K, Nishizawa T, Nureki O Science. 2019 Aug 15. pii: science.aay3353. doi: 10.1126/science.aay3353. PMID:31416931<ref>PMID:31416931</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6k7k" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: P-type phospholipid transporter]]
		+	[[Category: Hiraizumi, M]]
		+	[[Category: Nishizawa, T]]
		+	[[Category: Nureki, O]]
		+	[[Category: Yamashita, K]]
		+	[[Category: Flippase]]
		+	[[Category: Membrane protein]]

OCA at 03:59, 21 August 2019

2019-08-21T03:59:46Z

←Older revision		Revision as of 03:59, 21 August 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6k7k is ON HOLD	+	The entry 6k7k is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 04:32, 31 July 2019

2019-07-31T04:32:50Z

←Older revision		Revision as of 04:32, 31 July 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6k7k is ON HOLD ~~until Paper Publication~~	+	The entry 6k7k is ON HOLD

	Authors:		Authors:

OCA at 05:04, 26 June 2019

2019-06-26T05:04:29Z

←Older revision		Revision as of 05:04, 26 June 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6k7k is ON HOLD	+	The entry 6k7k is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6k7k" [edit=sysop:move=sysop]

2019-06-12T03:57:03Z

Protected "6k7k" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:57, 12 June 2019

OCA: New page: '''Unreleased structure''' The entry 6k7k is ON HOLD Authors: Description: Category: Unreleased Structures

2019-06-12T03:57:01Z

New page: '''Unreleased structure''' The entry 6k7k is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6k7k is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]