6kel - Revision history

OCA at 10:31, 22 November 2023

2023-11-22T10:31:55Z

←Older revision		Revision as of 10:31, 22 November 2023
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	<StructureSection load='6kel' size='340' side='right'caption='[[6kel]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='6kel' size='340' side='right'caption='[[6kel]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6kel]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Drome Drome~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KEL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6KEL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kel]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KEL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KEL FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE~~-1~~,3-DIOL'>TRS</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.4Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>GstE14, GSTD14-14, nobo, CG4688 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id~~=7227 DROME])</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-	~~<tr id='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[http~~:~~//en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=~~2~~.5.~~1~~.18 2.5.1.18]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kel OCA], [https://pdbe.org/6kel PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kel RCSB], [https://www.ebi.ac.uk/pdbsum/6kel PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kel ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6kel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kel OCA], [~~http~~://pdbe.org/6kel PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6kel RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6kel PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6kel ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/GSTEE_DROME GSTEE_DROME]] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles (PubMed:22082028). Essential for ecdysteroid biosynthesis (PubMed:25344753, PubMed:25300303). May be involved in detoxification (PubMed:22082028).<ref>PMID:22082028</ref> <ref>PMID:25300303</ref> <ref>PMID:25344753</ref>	+	[https://www.uniprot.org/uniprot/GSTEE_DROME GSTEE_DROME] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles (PubMed:22082028). Essential for ecdysteroid biosynthesis (PubMed:25344753, PubMed:25300303). May be involved in detoxification (PubMed:22082028).<ref>PMID:22082028</ref> <ref>PMID:25300303</ref> <ref>PMID:25344753</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 6kel" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6kel" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glutathione S-transferase 3D structures\|Glutathione S-transferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Drome]]~~	+	[[Category: Drosophila melanogaster]]
-	~~[[Category: Glutathione transferase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Inaba, K]]	+	[[Category: Inaba K]]
-	[[Category: Koiwai, K]]	+	[[Category: Koiwai K]]
-	[[Category: Morohashi, K]]	+	[[Category: Morohashi K]]
-	[[Category: Niwa, R]]	+	[[Category: Niwa R]]
-	[[Category: Senda, T]]	+	[[Category: Senda T]]
-	[[Category: Yumoto, F]]	+	[[Category: Yumoto F]]
-	~~[[Category: Glutathione]]~~	+
-	~~[[Category: Glutathione s-transferase]]~~	+
-	~~[[Category: Gst]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:10, 15 April 2020

2020-04-15T07:10:51Z

←Older revision		Revision as of 07:10, 15 April 2020
Line 3:		Line 3:
	<StructureSection load='6kel' size='340' side='right'caption='[[6kel]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='6kel' size='340' side='right'caption='[[6kel]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6kel]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KEL OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6KEL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kel]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KEL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KEL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GstE14, GSTD14-14, nobo, CG4688 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6kel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kel OCA], [http://pdbe.org/6kel PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kel RCSB], [http://www.ebi.ac.uk/pdbsum/6kel PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kel ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kel OCA], [http://pdbe.org/6kel PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kel RCSB], [http://www.ebi.ac.uk/pdbsum/6kel PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kel ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/GSTEE_DROME GSTEE_DROME]] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles (PubMed:22082028). Essential for ecdysteroid biosynthesis (PubMed:25344753, PubMed:25300303). May be involved in detoxification (PubMed:22082028).<ref>PMID:22082028</ref> <ref>PMID:25300303</ref> <ref>PMID:25344753</ref>		[[http://www.uniprot.org/uniprot/GSTEE_DROME GSTEE_DROME]] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles (PubMed:22082028). Essential for ecdysteroid biosynthesis (PubMed:25344753, PubMed:25300303). May be involved in detoxification (PubMed:22082028).<ref>PMID:22082028</ref> <ref>PMID:25300303</ref> <ref>PMID:25344753</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ecdysteroids are the principal steroid hormones essential for insect development and physiology. In the last 18 years, several enzymes responsible for ecdysteroid biosynthesis encoded by Halloween genes were identified and genetically and biochemically characterized. However, the tertiary structures of these proteins have not yet been characterized. Here, we report the results of an integrated series of in silico, in vitro, and in vivo analyses of the Halloween GST protein Noppera-bo (Nobo). We determined crystal structures of Drosophila melanogaster Nobo (DmNobo) complexed with glutathione and 17beta-estradiol, a DmNobo inhibitor. 17beta-Estradiol almost fully occupied the putative ligand-binding pocket and a prominent hydrogen bond formed between 17beta-estradiol and Asp-113 of DmNobo. We found that Asp-113 is essential for 17beta-estradiol-mediated inhibition of DmNobo enzymatic activity, as 17beta-estradiol did not inhibit and physically interacted less with the D113A DmNobo variant. Asp-113 is highly conserved among Nobo proteins, but not among other GSTs, implying that this residue is important for endogenous Nobo function. Indeed, a homozygous nobo allele with the D113A substitution exhibited embryonic lethality and an undifferentiated cuticle structure, a phenocopy of complete loss-of-function nobo homozygotes. These results suggest that the nobo family of GST proteins has acquired a unique amino acid residue that appears to be essential for binding an endogenous sterol substrate to regulate ecdysteroid biosynthesis. To the best of our knowledge, ours is the first study describing the structural characteristics of insect steroidogenic Halloween proteins. Our findings provide insights relevant for applied entomology to develop insecticides that specifically inhibit ecdysteroid biosynthesis.
		+
		+	An integrated approach to unravel a crucial structural property required for the function of the insect steroidogenic Halloween protein Noppera-bo.,Koiwai K, Inaba K, Morohashi K, Enya S, Arai R, Kojima H, Okabe T, Fujikawa Y, Inoue H, Yoshino R, Hirokawa T, Kato K, Fukuzawa K, Shimada-Niwa Y, Nakamura A, Yumoto F, Senda T, Niwa R J Biol Chem. 2020 Apr 2. pii: RA119.011463. doi: 10.1074/jbc.RA119.011463. PMID:32241910<ref>PMID:32241910</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6kel" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Drome]]
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 10:23, 2 October 2019

2019-10-02T10:23:17Z

←Older revision		Revision as of 10:23, 2 October 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6kel is ~~ON HOLD until Jul 04 2021~~	+	==Crystal structure of Drosophila melanogaster Noppera-bo, glutathione S-transferase epsilon 14 (DmGSTE14), in apo-form==
-		+	<StructureSection load='6kel' size='340' side='right'caption='[[6kel]], [[Resolution\|resolution]] 1.40Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6kel]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KEL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6KEL FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6kel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kel OCA], [http://pdbe.org/6kel PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kel RCSB], [http://www.ebi.ac.uk/pdbsum/6kel PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kel ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GSTEE_DROME GSTEE_DROME]] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles (PubMed:22082028). Essential for ecdysteroid biosynthesis (PubMed:25344753, PubMed:25300303). May be involved in detoxification (PubMed:22082028).<ref>PMID:22082028</ref> <ref>PMID:25300303</ref> <ref>PMID:25344753</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Glutathione transferase]]
		+	[[Category: Large Structures]]
		+	[[Category: Inaba, K]]
		+	[[Category: Koiwai, K]]
		+	[[Category: Morohashi, K]]
		+	[[Category: Niwa, R]]
		+	[[Category: Senda, T]]
		+	[[Category: Yumoto, F]]
		+	[[Category: Glutathione]]
		+	[[Category: Glutathione s-transferase]]
		+	[[Category: Gst]]
		+	[[Category: Transferase]]

OCA at 08:42, 17 July 2019

2019-07-17T08:42:14Z

←Older revision		Revision as of 08:42, 17 July 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6kel is ON HOLD	+	The entry 6kel is ON HOLD until Jul 04 2021

	Authors:		Authors:

OCA: Protected "6kel" [edit=sysop:move=sysop]

2019-07-10T04:03:22Z

Protected "6kel" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:03, 10 July 2019

OCA: New page: '''Unreleased structure''' The entry 6kel is ON HOLD Authors: Description: Category: Unreleased Structures

2019-07-10T04:03:20Z

New page: '''Unreleased structure''' The entry 6kel is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6kel is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]