6kfi - Revision history

OCA at 07:35, 1 May 2024

2024-05-01T07:35:44Z

←Older revision		Revision as of 07:35, 1 May 2024
Line 1:		Line 1:

	==NMR solution structure of the 1:1 complex of Tel26 G-quadruplex and a tripodal cationic fluorescent probe NBTE==		==NMR solution structure of the 1:1 complex of Tel26 G-quadruplex and a tripodal cationic fluorescent probe NBTE==
-	<StructureSection load='6kfi' size='340' side='right'caption='[[6kfi~~]], [[NMR_Ensembles_of_Models \| 15 NMR models~~]]' scene=''>	+	<StructureSection load='6kfi' size='340' side='right'caption='[[6kfi]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6kfi]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KFI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6KFI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kfi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KFI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=D6F:4,4,4-(nitrilotris(benzene-4,1-diyl))tris(1-ethylpyridin-1-ium)+iodide'>D6F</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6kfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kfi OCA], [~~http~~://pdbe.org/6kfi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6kfi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6kfi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6kfi ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=D6F:4,4,4-(nitrilotris(benzene-4,1-diyl))tris(1-ethylpyridin-1-ium)+iodide'>D6F</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kfi OCA], [https://pdbe.org/6kfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kfi RCSB], [https://www.ebi.ac.uk/pdbsum/6kfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kfi ProSAT]</span></td></tr>
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	G-quadruplex DNA show structural polymorphism, leading to challenges in the use of selective recognition probes for the accurate detection of G-quadruplexes in vivo. Herein, we present a tripodal cationic fluorescent probe, NBTE, which showed distinguishable fluorescence lifetime responses between G-quadruplexes and other DNA topologies, and fluorescence quantum yield (Phif ) enhancement upon G-quadruplex binding. We determined two NBTE-G-quadruplex complex structures with high Phif values by NMR spectroscopy. The structures indicated NBTE interacted with G-quadruplexes using three arms through pi-pi stacking, differing from that with duplex DNA using two arms, which rationalized the higher Phif values and lifetime response of NBTE upon G-quadruplex binding. Based on photon counts of FLIM, we detected the percentage of G-quadruplex DNA in live cells with NBTE and found G-quadruplex DNA content in cancer cells is 4-fold that in normal cells, suggesting the potential applications of this probe in cancer cell detection.
-
-	Quantitative Detection of G-Quadruplex DNA in Live Cells Based on Photon Counts and Complex Structure Discrimination.,Liu LY, Liu W, Wang KN, Zhu BC, Xia XY, Ji LN, Mao ZW Angew Chem Int Ed Engl. 2020 Jun 8;59(24):9719-9726. doi: 10.1002/anie.202002422., Epub 2020 Apr 1. PMID:32173994<ref>PMID:32173994</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 6kfi" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Liu~~, L Y~~]]	+	[[Category: Liu LY]]
-	[[Category: Liu, W]]	+	[[Category: Liu W]]
-	[[Category: Mao~~, Z W]]~~	+	[[Category: Mao ZW]]
-	~~[[Category: Dna]]~~	+
-	~~[[Category: G-quadruplex dna]]~~	+
-	~~[[Category: Probe~~]]	+

OCA at 11:22, 23 September 2020

2020-09-23T11:22:13Z

←Older revision		Revision as of 11:22, 23 September 2020
Line 1:		Line 1:

	==NMR solution structure of the 1:1 complex of Tel26 G-quadruplex and a tripodal cationic fluorescent probe NBTE==		==NMR solution structure of the 1:1 complex of Tel26 G-quadruplex and a tripodal cationic fluorescent probe NBTE==
-	<StructureSection load='6kfi' size='340' side='right'caption='[[6kfi]]' scene=''>	+	<StructureSection load='6kfi' size='340' side='right'caption='[[6kfi]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KFI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KFI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kfi]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KFI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KFI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kfi OCA], [http://pdbe.org/6kfi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kfi RCSB], [http://www.ebi.ac.uk/pdbsum/6kfi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kfi ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=D6F:4,4,4-(nitrilotris(benzene-4,1-diyl))tris(1-ethylpyridin-1-ium)+iodide'>D6F</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kfi OCA], [http://pdbe.org/6kfi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kfi RCSB], [http://www.ebi.ac.uk/pdbsum/6kfi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kfi ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	G-quadruplex DNA show structural polymorphism, leading to challenges in the use of selective recognition probes for the accurate detection of G-quadruplexes in vivo. Herein, we present a tripodal cationic fluorescent probe, NBTE, which showed distinguishable fluorescence lifetime responses between G-quadruplexes and other DNA topologies, and fluorescence quantum yield (Phif ) enhancement upon G-quadruplex binding. We determined two NBTE-G-quadruplex complex structures with high Phif values by NMR spectroscopy. The structures indicated NBTE interacted with G-quadruplexes using three arms through pi-pi stacking, differing from that with duplex DNA using two arms, which rationalized the higher Phif values and lifetime response of NBTE upon G-quadruplex binding. Based on photon counts of FLIM, we detected the percentage of G-quadruplex DNA in live cells with NBTE and found G-quadruplex DNA content in cancer cells is 4-fold that in normal cells, suggesting the potential applications of this probe in cancer cell detection.
		+
		+	Quantitative Detection of G-Quadruplex DNA in Live Cells Based on Photon Counts and Complex Structure Discrimination.,Liu LY, Liu W, Wang KN, Zhu BC, Xia XY, Ji LN, Mao ZW Angew Chem Int Ed Engl. 2020 Jun 8;59(24):9719-9726. doi: 10.1002/anie.202002422., Epub 2020 Apr 1. PMID:32173994<ref>PMID:32173994</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6kfi" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Liu LY]]	+	[[Category: Liu, L Y]]
-	[[Category: Liu W]]	+	[[Category: Liu, W]]
-	[[Category: Mao ZW]]	+	[[Category: Mao, Z W]]
		+	[[Category: Dna]]
		+	[[Category: G-quadruplex dna]]
		+	[[Category: Probe]]

OCA at 13:59, 8 July 2020

2020-07-08T13:59:12Z

←Older revision		Revision as of 13:59, 8 July 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6kfi is ON HOLD~~	+	==NMR solution structure of the 1:1 complex of Tel26 G-quadruplex and a tripodal cationic fluorescent probe NBTE==
-		+	<StructureSection load='6kfi' size='340' side='right'caption='[[6kfi]]' scene=''>
-	~~Authors: Liu, W., Liu, L.Y., Mao, Z.W.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KFI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KFI FirstGlance]. <br>
-	~~Description:~~ NMR solution structure of the 1:1 complex of Tel26 G-quadruplex and a tripodal cationic fluorescent probe NBTE	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kfi OCA], [http://pdbe.org/6kfi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kfi RCSB], [http://www.ebi.ac.uk/pdbsum/6kfi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kfi ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Mao~~, Z.W]]	+	__TOC__
-	[[Category: Liu~~, L.Y~~]]	+	</StructureSection>
-	[[Category: Liu, W]]	+	[[Category: Large Structures]]
		+	[[Category: Liu LY]]
		+	[[Category: Liu W]]
		+	[[Category: Mao ZW]]

OCA: Protected "6kfi" [edit=sysop:move=sysop]

2019-07-17T08:44:18Z

Protected "6kfi" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:44, 17 July 2019

OCA: New page: '''Unreleased structure''' The entry 6kfi is ON HOLD Authors: Liu, W., Liu, L.Y., Mao, Z.W. Description: NMR solution structure of the 1:1 complex of Tel26 G-quadruplex and a tripodal ...

2019-07-17T08:44:17Z

New page: '''Unreleased structure''' The entry 6kfi is ON HOLD Authors: Liu, W., Liu, L.Y., Mao, Z.W. Description: NMR solution structure of the 1:1 complex of Tel26 G-quadruplex and a tripodal ...

New page

'''Unreleased structure'''

The entry 6kfi is ON HOLD

Authors: Liu, W., Liu, L.Y., Mao, Z.W.

Description: NMR solution structure of the 1:1 complex of Tel26 G-quadruplex and a tripodal cationic fluorescent probe NBTE
[[Category: Unreleased Structures]]
[[Category: Mao, Z.W]]
[[Category: Liu, L.Y]]
[[Category: Liu, W]]