6kmy - Revision history

OCA at 10:53, 14 June 2023

2023-06-14T10:53:26Z

←Older revision		Revision as of 10:53, 14 June 2023
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	==Structure of single disulfide peptide Czon1107-P5A==		==Structure of single disulfide peptide Czon1107-P5A==
-	<StructureSection load='6kmy' size='340' side='right'caption='[[6kmy~~]], [[NMR_Ensembles_of_Models \| 28 NMR models~~]]' scene=''>	+	<StructureSection load='6kmy' size='340' side='right'caption='[[6kmy]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6kmy]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KMY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6KMY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kmy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Conus_zonatus Conus zonatus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KMY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KMY FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6kmy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kmy OCA], [~~http~~://pdbe.org/6kmy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6kmy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6kmy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6kmy ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kmy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kmy OCA], [https://pdbe.org/6kmy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kmy RCSB], [https://www.ebi.ac.uk/pdbsum/6kmy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kmy ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Conus zonatus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kumar, M ~~Madhan~~]]	+	[[Category: Madhan Kumar M]]
-	[[Category: Sarma~~, S P]]~~	+	[[Category: Sarma SP]]
-	~~[[Category: Nachr anatgonist nmr]]~~	+
-	~~[[Category: Neuropeptide~~]]	+

OCA at 06:01, 22 April 2020

2020-04-22T06:01:15Z

←Older revision		Revision as of 06:01, 22 April 2020
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kmy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kmy OCA], [http://pdbe.org/6kmy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kmy RCSB], [http://www.ebi.ac.uk/pdbsum/6kmy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kmy ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kmy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kmy OCA], [http://pdbe.org/6kmy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kmy RCSB], [http://www.ebi.ac.uk/pdbsum/6kmy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kmy ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Conopeptides are neurotoxic peptides in the venom of marine cone snails and have broad therapeutic potential for managing pain and other conditions. Here, we identified the single-disulfide peptides Czon1107 and Cca1669 from the venoms of Conus zonatus and Conus caracteristicus, respectively. We observed that Czon1107 strongly inhibits the human alpha3beta4 (IC50 15.7 +/- 3.0 muM) and alpha7 (IC50 77.1 +/- 0.05 muM) nicotinic acetylcholine receptor (nAChR) subtypes, but the activity of Cca1669 remains to be identified. Czon1107 acted at a site distinct from the orthosteric receptor site. Solution NMR experiments revealed that Czon1107 exists in equilibrium between conformational states that are the result of a key Ser(4)-Pro(5) cis-trans isomerization. Moreover, we found that the X-Pro amide bonds in the inter-cysteine loop are rigidly constrained to cis conformations. Structure-activity experiments of Czon1107 and its variants at positions P5 and P7 revealed that the conformation around the X-Pro bonds (cis-trans) plays an important role in receptor subtype selectivity. The cis conformation at the Cys(6)-Pro(7) peptide bond was essential for alpha3beta4 nAChR subtype allosteric selectivity. In summary, we have identified an unique single disulfide conopeptide with a non-competitive, potentially allosteric inhibitory mechanism at the nAChRs. The small size and rigidity of the Czon1107 peptide could provide a scaffold for rational drug design strategies for allosteric nAChR modulation. This new paradigm in the "conotoxinomic" structure-function space provides an impetus to screen venom from other Conus species for similar, short bioactive peptides that allosterically modulate ligand-gated receptor function.
		+
		+	Structure and allosteric activity of a single disulfide conopeptide from Conus zonatus at human alpha3beta4 and alpha7 nicotinic acetylcholine receptors.,Mohan MK, Abraham N, R P R, Jayaseelan BF, Ragnarsson L, Lewis RJ, Sarma SP J Biol Chem. 2020 Mar 31. pii: RA119.012098. doi: 10.1074/jbc.RA119.012098. PMID:32234761<ref>PMID:32234761</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6kmy" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:47, 8 April 2020

2020-04-08T06:47:45Z

←Older revision		Revision as of 06:47, 8 April 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6kmy is ~~ON HOLD until Paper Publication~~	+	==Structure of single disulfide peptide Czon1107-P5A==
-		+	<StructureSection load='6kmy' size='340' side='right'caption='[[6kmy]], [[NMR_Ensembles_of_Models \| 28 NMR models]]' scene=''>
-	~~Authors~~: ~~Sarma~~, S.P., ~~Madhan Kumar~~, M.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6kmy]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KMY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KMY FirstGlance]. <br>
-	~~Description~~: ~~Structure of single disulfide peptide Czon1107~~-~~P5A~~	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kmy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kmy OCA], [http://pdbe.org/6kmy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kmy RCSB], [http://www.ebi.ac.uk/pdbsum/6kmy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kmy ProSAT]</span></td></tr>
-	[[Category: Sarma, S.P]]	+	</table>
-	[[Category: ~~Madhan Kumar, M~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Kumar, M Madhan]]
		+	[[Category: Sarma, S P]]
		+	[[Category: Nachr anatgonist nmr]]
		+	[[Category: Neuropeptide]]

OCA at 08:13, 27 March 2020

2020-03-27T08:13:09Z

←Older revision		Revision as of 08:13, 27 March 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6kmy is ON HOLD	+	The entry 6kmy is ON HOLD until Paper Publication

	Authors: Sarma, S.P., Madhan Kumar, M.		Authors: Sarma, S.P., Madhan Kumar, M.

OCA at 05:25, 4 March 2020

2020-03-04T05:25:31Z

←Older revision		Revision as of 05:25, 4 March 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6kmy is ON HOLD ~~until Paper Publication~~	+	The entry 6kmy is ON HOLD

	Authors: Sarma, S.P., Madhan Kumar, M.		Authors: Sarma, S.P., Madhan Kumar, M.

OCA at 06:34, 2 October 2019

2019-10-02T06:34:54Z

←Older revision		Revision as of 06:34, 2 October 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6kmy is ON HOLD	+	The entry 6kmy is ON HOLD until Paper Publication

	Authors: Sarma, S.P., Madhan Kumar, M.		Authors: Sarma, S.P., Madhan Kumar, M.

OCA: Protected "6kmy" [edit=sysop:move=sysop]

2019-08-07T04:13:00Z

Protected "6kmy" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:13, 7 August 2019

OCA: New page: '''Unreleased structure''' The entry 6kmy is ON HOLD Authors: Sarma, S.P., Madhan Kumar, M. Description: Structure of single disulfide peptide Czon1107-P5A [[Category: Unreleased Struc...

2019-08-07T04:12:59Z

New page: '''Unreleased structure''' The entry 6kmy is ON HOLD Authors: Sarma, S.P., Madhan Kumar, M. Description: Structure of single disulfide peptide Czon1107-P5A [[Category: Unreleased Struc...

New page

'''Unreleased structure'''

The entry 6kmy is ON HOLD

Authors: Sarma, S.P., Madhan Kumar, M.

Description: Structure of single disulfide peptide Czon1107-P5A
[[Category: Unreleased Structures]]
[[Category: Sarma, S.P]]
[[Category: Madhan Kumar, M]]