OCA at 10:49, 22 November 2023

2023-11-22T10:49:33Z

←Older revision		Revision as of 10:49, 22 November 2023
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	==Crystal structure of DUSP22 mutant_C88S==		==Crystal structure of DUSP22 mutant_C88S==
-	<StructureSection load='6l1s' size='340' side='right'caption='[[6l1s]]' scene=''>	+	<StructureSection load='6l1s' size='340' side='right'caption='[[6l1s]], [[Resolution\|resolution]] 1.36Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L1S OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6L1S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6l1s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L1S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L1S FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6l1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l1s OCA], [~~http~~://pdbe.org/6l1s PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6l1s RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6l1s PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6l1s ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.3611Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l1s OCA], [https://pdbe.org/6l1s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l1s RCSB], [https://www.ebi.ac.uk/pdbsum/6l1s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l1s ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/DUS22_HUMAN DUS22_HUMAN] Activates the Jnk signaling pathway. Dephosphorylates and deactivates p38 and stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) (By similarity).<ref>PMID:11717427</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cysteine-based protein tyrosine phosphatases (Cys-based PTPs) perform dephosphorylation to regulate signaling pathways in cellular responses. The hydrogen bonding network in their active site plays an important conformational role and supports the phosphatase activity. Nearly half of dual-specificity phosphatases (DUSPs) use three conserved residues, including aspartate in the D-loop, serine in the P-loop, and asparagine in the N-loop, to form the hydrogen bonding network, the D-, P-, N-triloop interaction (DPN-triloop interaction). In this study, DUSP22 is used to investigate the importance of the DPN-triloop interaction in active site formation. Alanine mutations and somatic mutations of the conserved residues, D57, S93, and N128 substantially decrease catalytic efficiency (kcat/KM) by more than 10(2)-fold. Structural studies by NMR and crystallography reveal that each residue can perturb the three loops and induce conformational changes, indicating that the hydrogen bonding network aligns the residues in the correct positions for substrate interaction and catalysis. Studying the DPN-triloop interaction reveals the mechanism maintaining phosphatase activity in N-loop-containing PTPs and provides a foundation for further investigation of active site formation in different members of this protein class.
		+
		+	Structural Insights into the Active Site Formation of DUSP22 in N-loop-containing Protein Tyrosine Phosphatases.,Lai CH, Chang CC, Chuang HC, Tan TH, Lyu PC Int J Mol Sci. 2020 Oct 12;21(20). pii: ijms21207515. doi: 10.3390/ijms21207515. PMID:33053837<ref>PMID:33053837</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6l1s" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Dual specificity phosphatase 3D structures\|Dual specificity phosphatase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Chang CC]]		[[Category: Chang CC]]
	[[Category: Lai CH]]		[[Category: Lai CH]]
	[[Category: Lyu PC]]		[[Category: Lyu PC]]

OCA at 20:30, 28 October 2020

2020-10-28T20:30:55Z

←Older revision		Revision as of 20:30, 28 October 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6l1s is ~~ON HOLD until Oct 30 2021~~	+	==Crystal structure of DUSP22 mutant_C88S==
-		+	<StructureSection load='6l1s' size='340' side='right'caption='[[6l1s]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L1S OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6L1S FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6l1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l1s OCA], [http://pdbe.org/6l1s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l1s RCSB], [http://www.ebi.ac.uk/pdbsum/6l1s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l1s ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Chang CC]]
		+	[[Category: Lai CH]]
		+	[[Category: Lyu PC]]

OCA at 08:05, 12 August 2020

2020-08-12T08:05:13Z

←Older revision		Revision as of 08:05, 12 August 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6l1s is ON HOLD until ~~Paper Publication~~	+	The entry 6l1s is ON HOLD until Oct 30 2021

	Authors:		Authors:

OCA at 07:18, 30 October 2019

2019-10-30T07:18:38Z

←Older revision		Revision as of 07:18, 30 October 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6l1s is ON HOLD	+	The entry 6l1s is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6l1s" [edit=sysop:move=sysop]

2019-10-10T03:35:28Z

Protected "6l1s" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:35, 10 October 2019

OCA: New page: '''Unreleased structure''' The entry 6l1s is ON HOLD Authors: Description: Category: Unreleased Structures

2019-10-10T03:35:27Z

New page: '''Unreleased structure''' The entry 6l1s is ON HOLD Authors: Description: Category: Unreleased Structures

New page