6l32 - Revision history

OCA at 10:50, 22 November 2023

2023-11-22T10:50:45Z

←Older revision		Revision as of 10:50, 22 November 2023
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	==Crystal structure of potassium induced heme modification in yak lactoperoxidase at 2.30 A resolution==		==Crystal structure of potassium induced heme modification in yak lactoperoxidase at 2.30 A resolution==
-	<StructureSection load='6l32' size='340' side='right'caption='[[6l32]]' scene=''>	+	<StructureSection load='6l32' size='340' side='right'caption='[[6l32]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L32 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L32 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6l32]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_mutus Bos mutus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L32 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L32 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l32 OCA], [https://pdbe.org/6l32 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l32 RCSB], [https://www.ebi.ac.uk/pdbsum/6l32 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l32 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l32 OCA], [https://pdbe.org/6l32 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l32 RCSB], [https://www.ebi.ac.uk/pdbsum/6l32 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l32 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/L8ICE9_9CETA L8ICE9_9CETA]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lactoperoxidase, a heme-containing glycoprotein, catalyzes the oxidation of thiocyanate by hydrogen peroxide into hypothiocyanite which acts as an antibacterial agent. The prosthetic heme moiety is attached to the protein through two ester linkages via Glu258 and Asp108. In lactoperoxidase, the substrate-binding site is formed on the distal heme side. To study the effect of physiologically important potassium ion on the structure and function of lactoperoxidase, the fresh protein samples were isolated from yak (Bos grunniens) colostrum and purified to homogeneity. The biochemical studies with potassium fluoride showed a significant reduction in the catalytic activity. Lactoperoxidase was crystallized using 200 mM ammonium nitrate and 20% PEG-3350 at pH 6.0. The crystals of LPO were soaked in the solution of potassium fluoride and used for the X-ray intensity data collection. Structure determination at 2.20 A resolution revealed the presence of a potassium ion in the distal heme cavity. Structure determination further revealed that the propionic chain attached to pyrrole ring C of the heme moiety, was disordered into two components each having an occupancy of 0.5. One component occupied a position similar to the normally observed position of propionic chain while the second component was found in the distal heme cavity. The potassium ion in the distal heme cavity formed five coordinate bonds with two oxygen atoms of propionic moiety, N(epsilon2) atom of His109 and two oxygen atoms of water molecules. The presence of potassium ion in the distal heme cavity hampered the catalytic activity of lactoperoxidase.
		+
		+	Potassium-induced partial inhibition of lactoperoxidase: structure of the complex of lactoperoxidase with potassium ion at 2.20 A resolution.,Singh PK, Pandey S, Rani C, Ahmad N, Viswanathan V, Sharma P, Kaur P, Sharma S, Singh TP J Biol Inorg Chem. 2021 Feb;26(1):149-159. doi: 10.1007/s00775-020-01844-6. Epub , 2021 Jan 11. PMID:33427997<ref>PMID:33427997</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6l32" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Lactoperoxidase\|Lactoperoxidase]]		*[[Lactoperoxidase\|Lactoperoxidase]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bos mutus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Rani C]]		[[Category: Rani C]]

OCA at 08:27, 21 April 2021

2021-04-21T08:27:34Z

←Older revision		Revision as of 08:27, 21 April 2021
Line 1:		Line 1:

	==Crystal structure of potassium induced heme modification in yak lactoperoxidase at 2.30 A resolution==		==Crystal structure of potassium induced heme modification in yak lactoperoxidase at 2.30 A resolution==
-	<StructureSection load='6l32' size='340' side='right'caption='[[6l32]]~~, [[Resolution\|resolution]] 2.30Å~~' scene=''>	+	<StructureSection load='6l32' size='340' side='right'caption='[[6l32]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>~~[[6l32]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_mutus Bos mutus].~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L32 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6L32 FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L32 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L32 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l32 OCA], [https://pdbe.org/6l32 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l32 RCSB], [https://www.ebi.ac.uk/pdbsum/6l32 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l32 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6l32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l32 OCA], [~~http~~://pdbe.org/6l32 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6l32 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6l32 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6l32 ProSAT]</span></td></tr>	+
	</table>		</table>
		+
		+	==See Also==
		+	*[[Lactoperoxidase\|Lactoperoxidase]]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bos mutus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Rani, C]]	+	[[Category: Rani C]]
-	[[Category: Sharma, P]]	+	[[Category: Sharma P]]
-	[[Category: Sharma, S]]	+	[[Category: Sharma S]]
-	[[Category: Singh~~, P K~~]]	+	[[Category: Singh PK]]
-	[[Category: Singh~~, T P]]~~	+	[[Category: Singh TP]]
-	~~[[Category: Lactoperoxidase]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 06:52, 23 October 2019

2019-10-23T06:52:25Z

←Older revision		Revision as of 06:52, 23 October 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6l32 ~~is ON HOLD~~	+	==Crystal structure of potassium induced heme modification in yak lactoperoxidase at 2.30 A resolution==
-		+	<StructureSection load='6l32' size='340' side='right'caption='[[6l32]], [[Resolution\|resolution]] 2.30Å' scene=''>
-	~~Authors~~: ~~Singh, P~~.K., ~~Rani~~, C., ~~Sharma~~, P., ~~Sharma~~, S., ~~Singh~~, T.P.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6l32]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_mutus Bos mutus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L32 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6L32 FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of potassium induced heme modification in yak lactoperoxidase at 2~~.~~30 A resolution~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6l32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l32 OCA], [http://pdbe.org/6l32 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l32 RCSB], [http://www.ebi.ac.uk/pdbsum/6l32 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l32 ProSAT]</span></td></tr>
-	[[Category: ~~Singh, P.K~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bos mutus]]
		+	[[Category: Large Structures]]
	[[Category: Rani, C]]		[[Category: Rani, C]]
		+	[[Category: Sharma, P]]
	[[Category: Sharma, S]]		[[Category: Sharma, S]]
-	[[Category: ~~Sharma~~, P]]	+	[[Category: Singh, P K]]
-	[[Category: Singh, T.P]]	+	[[Category: Singh, T P]]
		+	[[Category: Lactoperoxidase]]
		+	[[Category: Oxidoreductase]]

OCA: Protected "6l32" [edit=sysop:move=sysop]

2019-10-16T04:19:38Z

Protected "6l32" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:19, 16 October 2019

OCA: New page: '''Unreleased structure''' The entry 6l32 is ON HOLD Authors: Singh, P.K., Rani, C., Sharma, P., Sharma, S., Singh, T.P. Description: Crystal structure of potassium induced heme modifi...

2019-10-16T04:19:37Z

New page: '''Unreleased structure''' The entry 6l32 is ON HOLD Authors: Singh, P.K., Rani, C., Sharma, P., Sharma, S., Singh, T.P. Description: Crystal structure of potassium induced heme modifi...

New page

'''Unreleased structure'''

The entry 6l32 is ON HOLD

Authors: Singh, P.K., Rani, C., Sharma, P., Sharma, S., Singh, T.P.

Description: Crystal structure of potassium induced heme modification in yak lactoperoxidase at 2.30 A resolution
[[Category: Unreleased Structures]]
[[Category: Singh, P.K]]
[[Category: Rani, C]]
[[Category: Sharma, S]]
[[Category: Sharma, P]]
[[Category: Singh, T.P]]