OCA at 12:24, 9 June 2021

2021-06-09T12:24:00Z

←Older revision		Revision as of 12:24, 9 June 2021
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	==The structure of PRRSV helicase==		==The structure of PRRSV helicase==
-	<StructureSection load='6lkx' size='340' side='right'caption='[[6lkx]]' scene=''>	+	<StructureSection load='6lkx' size='340' side='right'caption='[[6lkx]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LKX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6LKX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6lkx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Prrsv Prrsv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LKX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LKX FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6lkx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lkx OCA], [~~http~~://pdbe.org/6lkx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6lkx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6lkx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6lkx ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lkx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lkx OCA], [https://pdbe.org/6lkx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lkx RCSB], [https://www.ebi.ac.uk/pdbsum/6lkx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lkx ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Currently, an effective therapeutic treatment for porcine reproductive and respiratory syndrome virus (PRRSV) remains elusive. PRRSV helicase nsp10 is an important component of the replication transcription complex that plays a crucial role in viral replication, making nsp10 an important target for drug development. Here, we report the first crystal structure of full-length nsp10 from the arterivirus PRRSV, which has multiple domains: an N-terminal zinc-binding domain (ZBD), a 1B domain, and helicase core domains 1A and 2A. Importantly, our structural analyses indicate that the conformation of the 1B domain from arterivirus nsp10 undergoes a dynamic transition. The polynucleotide substrate channel formed by domains 1A and 1B adopts an open state, which may create enough space to accommodate and bind double-stranded RNA (dsRNA) during unwinding. Moreover, we report a unique C-terminal domain structure that participates in stabilizing the overall helicase structure. Our biochemical experiments also showed that deletion of the 1B domain and C-terminal domain significantly reduced the helicase activity of nsp10, indicating that the four domains must cooperate to contribute to helicase function. In addition, our results indicate that nidoviruses contain a conserved helicase core domain and key amino acid sites affecting helicase function, which share a common mechanism of helicase translocation and unwinding activity. These findings will help to further our understanding of the mechanism of helicase function and provide new targets for the development of antiviral drugs.IMPORTANCE Porcine reproductive and respiratory syndrome virus (PRRSV) is a major respiratory disease agent in pigs that causes enormous economic losses to the global swine industry. PRRSV helicase nsp10 is a multifunctional protein with translocation and unwinding activities and plays a vital role in viral RNA synthesis. Here, we report the first structure of full-length nsp10 from the arterivirus PRRSV at 3.0-A resolution. Our results show that the 1B domain of PRRSV nsp10 adopts a novel open state and has a unique C-terminal domain structure, which plays a crucial role in nsp10 helicase activity. Furthermore, mutagenesis and structural analysis revealed conservation of the helicase catalytic domain across the order Nidovirales (families Arteriviridae and Coronaviridae). Importantly, our results will provide a structural basis for further understanding the function of helicases in the order Nidovirales.
		+
		+	Structural Characterization of the Helicase nsp10 Encoded by Porcine Reproductive and Respiratory Syndrome Virus.,Shi Y, Tong X, Ye G, Xiu R, Li L, Sun L, Shi J, Li M, Song Y, Fan C, Shi K, Fu ZF, Xiao S, Peng G J Virol. 2020 Jul 16;94(15). pii: JVI.02158-19. doi: 10.1128/JVI.02158-19. Print , 2020 Jul 16. PMID:32461315<ref>PMID:32461315</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6lkx" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[RNA polymerase 3D structures\|RNA polymerase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Peng GQ]]	+	[[Category: Prrsv]]
-	[[Category: Shi YJ]]	+	[[Category: Peng, G Q]]
-	[[Category: Tong XH]]	+	[[Category: Shi, Y J]]
		+	[[Category: Tong, X H]]
		+	[[Category: Helicase]]
		+	[[Category: Nsp10]]
		+	[[Category: Virus]]

OCA at 06:53, 27 May 2020

2020-05-27T06:53:29Z

←Older revision		Revision as of 06:53, 27 May 2020
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-	'''Unreleased structure'''

-	The ~~entry~~ 6lkx is ~~ON HOLD until Paper Publication~~	+	==The structure of PRRSV helicase==
-		+	<StructureSection load='6lkx' size='340' side='right'caption='[[6lkx]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LKX OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LKX FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lkx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lkx OCA], [http://pdbe.org/6lkx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lkx RCSB], [http://www.ebi.ac.uk/pdbsum/6lkx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lkx ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Peng GQ]]
		+	[[Category: Shi YJ]]
		+	[[Category: Tong XH]]

OCA at 04:08, 20 May 2020

2020-05-20T04:08:30Z

←Older revision		Revision as of 04:08, 20 May 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6lkx is ON HOLD	+	The entry 6lkx is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 03:47, 13 May 2020

2020-05-13T03:47:46Z

←Older revision		Revision as of 03:47, 13 May 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6lkx is ON HOLD ~~until Paper Publication~~	+	The entry 6lkx is ON HOLD

	Authors:		Authors:

OCA at 06:03, 5 February 2020

2020-02-05T06:03:23Z

←Older revision		Revision as of 06:03, 5 February 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6lkx is ON HOLD	+	The entry 6lkx is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6lkx" [edit=sysop:move=sysop]

2020-01-01T06:35:35Z

Protected "6lkx" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:35, 1 January 2020

OCA: New page: '''Unreleased structure''' The entry 6lkx is ON HOLD Authors: Description: Category: Unreleased Structures

2020-01-01T06:35:33Z

New page: '''Unreleased structure''' The entry 6lkx is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6lkx is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]