6lpf - Revision history

OCA at 14:42, 29 November 2023

2023-11-29T14:42:57Z

←Older revision		Revision as of 14:42, 29 November 2023
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	<StructureSection load='6lpf' size='340' side='right'caption='[[6lpf]], [[Resolution\|resolution]] 2.49Å' scene=''>		<StructureSection load='6lpf' size='340' side='right'caption='[[6lpf]], [[Resolution\|resolution]] 2.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6lpf]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LPF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6LPF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6lpf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LPF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LPF FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LSS:5~~-~~O-(L-LEUCYLSULFAMOYL)ADENOSINE'>LSS</scene>~~, ~~<scene name='pdbligand=VRT:~~2~~-(L-NORVALYL)AMINO-2-DEOXYADENOSINE'>VRT</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.49Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~LARS, KIAA1352 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=9606 HUMAN])</td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LSS:5-O-(L-LEUCYLSULFAMOYL)ADENOSINE'>LSS</scene>, <scene name='pdbligand=VRT:2-(L-NORVALYL)AMINO-2-DEOXYADENOSINE'>VRT</scene></td></tr>
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[http~~:~~//en.wikipedia.org/wiki/Leucine~~--~~tRNA_ligase Leucine~~--~~tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.4 6.1.1.4]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lpf OCA], [https://pdbe.org/6lpf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lpf RCSB], [https://www.ebi.ac.uk/pdbsum/6lpf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lpf ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6lpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lpf OCA], [~~http~~://pdbe.org/6lpf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6lpf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6lpf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6lpf ProSAT]</span></td></tr>	+
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/SYLC_HUMAN SYLC_HUMAN]] Acute infantile liver failure-multisystemic involvement syndrome. The disease is caused by mutations affecting the gene represented in this entry.	+	[https://www.uniprot.org/uniprot/SYLC_HUMAN SYLC_HUMAN] Acute infantile liver failure-multisystemic involvement syndrome. The disease is caused by mutations affecting the gene represented in this entry.
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SYLC_HUMAN SYLC_HUMAN]] Catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs.<ref>PMID:19426743</ref>	+	[https://www.uniprot.org/uniprot/SYLC_HUMAN SYLC_HUMAN] Catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs.<ref>PMID:19426743</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6lpf" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6lpf" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Aminoacyl tRNA synthetase 3D structures\|Aminoacyl tRNA synthetase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Leucine--tRNA ligase]]~~	+	[[Category: Cusack S]]
-	[[Category: Cusack, S]]	+	[[Category: Li H]]
-	[[Category: Li, H]]	+	[[Category: Li J]]
-	[[Category: Li, J]]	+	[[Category: Lin JZ]]
-	[[Category: Lin~~, J Z~~]]	+	[[Category: Liu RJ]]
-	[[Category: Liu~~, R J~~]]	+	[[Category: Long T]]
-	[[Category: Long, T]]	+	[[Category: Palencia A]]
-	[[Category: Palencia, A]]	+	[[Category: Wang ED]]
-	[[Category: Wang~~, E D~~]]	+	[[Category: Wang MZ]]
-	[[Category: Wang~~, M Z~~]]	+	[[Category: Zhao JH]]
-	[[Category: Zhao~~, J H]]~~	+
-	~~[[Category: Aminoacylation]]~~	+
-	~~[[Category: Leur]]~~	+
-	~~[[Category: Protein translation]]~~	+
-	~~[[Category: Rna binding protein]]~~	+
-	~~[[Category: Trna~~]]	+

OCA at 07:12, 15 April 2020

2020-04-15T07:12:49Z

←Older revision		Revision as of 07:12, 15 April 2020
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	<StructureSection load='6lpf' size='340' side='right'caption='[[6lpf]], [[Resolution\|resolution]] 2.49Å' scene=''>		<StructureSection load='6lpf' size='340' side='right'caption='[[6lpf]], [[Resolution\|resolution]] 2.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6lpf]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LPF OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6LPF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6lpf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LPF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LPF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LSS:5-O-(L-LEUCYLSULFAMOYL)ADENOSINE'>LSS</scene>, <scene name='pdbligand=VRT:2-(L-NORVALYL)AMINO-2-DEOXYADENOSINE'>VRT</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LSS:5-O-(L-LEUCYLSULFAMOYL)ADENOSINE'>LSS</scene>, <scene name='pdbligand=VRT:2-(L-NORVALYL)AMINO-2-DEOXYADENOSINE'>VRT</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LARS, KIAA1352 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Leucine--tRNA_ligase Leucine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.4 6.1.1.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Leucine--tRNA_ligase Leucine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.4 6.1.1.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6lpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lpf OCA], [http://pdbe.org/6lpf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lpf RCSB], [http://www.ebi.ac.uk/pdbsum/6lpf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lpf ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lpf OCA], [http://pdbe.org/6lpf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lpf RCSB], [http://www.ebi.ac.uk/pdbsum/6lpf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lpf ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 12:		Line 13:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/SYLC_HUMAN SYLC_HUMAN]] Catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs.<ref>PMID:19426743</ref>		[[http://www.uniprot.org/uniprot/SYLC_HUMAN SYLC_HUMAN]] Catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs.<ref>PMID:19426743</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Human cytosolic leucyl-tRNA synthetase (hcLRS) is an essential and multifunctional enzyme. Its canonical function is to catalyze the covalent ligation of leucine to tRNALeu, and it may also hydrolyze mischarged tRNAs through an editing mechanism. Together with eight other aminoacyl-tRNA synthetases (AaRSs) and three auxiliary proteins, it forms a large multi-synthetase complex (MSC). Beyond its role in translation, hcLRS has an important moonlight function as a leucine sensor in the rapamycin complex 1 (mTORC1) pathway. Since this pathway is active in cancer development, hcLRS is a potential target for anti-tumor drug development. Moreover, LRS from pathogenic microbes are proven drug targets for developing antibiotics, which however should not inhibit hcLRS. Here we present the crystal structure of hcLRS at a 2.5 A resolution, the first complete structure of a eukaryotic LRS, and analyze the binding of various compounds that target different sites of hcLRS. We also deduce the assembly mechanism of hcLRS into the MSC through reconstitution of the entire mega complex in vitro. Overall, our study provides the molecular basis for understanding both the multifaceted functions of hcLRS and for drug development targeting these functions.
		+
		+	Molecular basis of the multifaceted functions of human leucyl-tRNA synthetase in protein synthesis and beyond.,Liu RJ, Long T, Li H, Zhao J, Li J, Wang M, Palencia A, Lin J, Cusack S, Wang ED Nucleic Acids Res. 2020 Mar 30. pii: 5813801. doi: 10.1093/nar/gkaa189. PMID:32232361<ref>PMID:32232361</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6lpf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Leucine--tRNA ligase]]		[[Category: Leucine--tRNA ligase]]

OCA at 10:09, 27 March 2020

2020-03-27T10:09:20Z

←Older revision		Revision as of 10:09, 27 March 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The ~~entry~~ 6lpf is ~~ON HOLD until Paper Publication~~	+	==The crystal structure of human cytoplasmic LRS==
-		+	<StructureSection load='6lpf' size='340' side='right'caption='[[6lpf]], [[Resolution\|resolution]] 2.49Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6lpf]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LPF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6LPF FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LSS:5-O-(L-LEUCYLSULFAMOYL)ADENOSINE'>LSS</scene>, <scene name='pdbligand=VRT:2-(L-NORVALYL)AMINO-2-DEOXYADENOSINE'>VRT</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Leucine--tRNA_ligase Leucine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.4 6.1.1.4] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6lpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lpf OCA], [http://pdbe.org/6lpf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lpf RCSB], [http://www.ebi.ac.uk/pdbsum/6lpf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lpf ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/SYLC_HUMAN SYLC_HUMAN]] Acute infantile liver failure-multisystemic involvement syndrome. The disease is caused by mutations affecting the gene represented in this entry.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SYLC_HUMAN SYLC_HUMAN]] Catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs.<ref>PMID:19426743</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Leucine--tRNA ligase]]
		+	[[Category: Cusack, S]]
		+	[[Category: Li, H]]
		+	[[Category: Li, J]]
		+	[[Category: Lin, J Z]]
		+	[[Category: Liu, R J]]
		+	[[Category: Long, T]]
		+	[[Category: Palencia, A]]
		+	[[Category: Wang, E D]]
		+	[[Category: Wang, M Z]]
		+	[[Category: Zhao, J H]]
		+	[[Category: Aminoacylation]]
		+	[[Category: Leur]]
		+	[[Category: Protein translation]]
		+	[[Category: Rna binding protein]]
		+	[[Category: Trna]]

OCA: Protected "6lpf" [edit=sysop:move=sysop]

2020-01-22T13:37:03Z

Protected "6lpf" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:37, 22 January 2020

OCA: New page: '''Unreleased structure''' The entry 6lpf is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

2020-01-22T13:37:01Z

New page: '''Unreleased structure''' The entry 6lpf is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6lpf is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]