OCA at 14:44, 29 November 2023

2023-11-29T14:44:48Z

←Older revision		Revision as of 14:44, 29 November 2023
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	==structure of Spermidine hydroxycinnamoyl transferases from Arabidopsis thaliana==		==structure of Spermidine hydroxycinnamoyl transferases from Arabidopsis thaliana==
-	<StructureSection load='6lpv' size='340' side='right'caption='[[6lpv]]' scene=''>	+	<StructureSection load='6lpv' size='340' side='right'caption='[[6lpv]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LPV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6LPV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6lpv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LPV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LPV FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6lpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lpv OCA], [~~http~~://pdbe.org/6lpv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6lpv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6lpv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6lpv ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.297Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=SPD:SPERMIDINE'>SPD</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lpv OCA], [https://pdbe.org/6lpv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lpv RCSB], [https://www.ebi.ac.uk/pdbsum/6lpv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lpv ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SHT_ARATH SHT_ARATH] Hydroxycinnamoyl transferase involved in the conjugation of feruloyl CoA to spermidine (PubMed:19077165, PubMed:19762055, PubMed:33519864). Catalyzes the three conjugating steps required for the biosynthesis of N(1),N(4),N(8)-triferuloyl-spermidine (PubMed:19077165, PubMed:33519864). Spermidine is the only acceptor substrate while feruloyl CoA > caffeoyl CoA > coumaroyl CoA > cinnamoyl CoA >> sinapoyl CoA are efficient acyl donors. No activity with hydroxyferuloyl CoA (PubMed:19077165). Required for the biosynthesis of these conjugated spermidine derivatives, specifically in anther tapetum (PubMed:22912643).<ref>PMID:19077165</ref> <ref>PMID:19762055</ref> <ref>PMID:22912643</ref> <ref>PMID:33519864</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Phenolamides represent one of the largest classes of plant-specialized secondary metabolites and function in diverse physiological processes, including defense responses and development. The biosynthesis of phenolamides requires the BAHD-family acyltransferases, which transfer acyl-groups from different acyl-donors specifically to amines, the acyl-group acceptors. However, the mechanisms of substrate specificity and multisite-acylation of the BAHD-family acyltransferases remain poorly understood. In this study, we provide a structural and biochemical analysis of AtSHT and AtSDT, two representative BAHD-family members that catalyze the multisite acylation of spermidine but show different product profiles. By determining the structures of AtSHT and AtSDT and using structure-based mutagenesis, we identified the residues important for substrate recognition in AtSHT and AtSDT and hypothesized that the acyl acceptor spermidine might adopt a free-rotating conformation in AtSHT, which can undergo mono-, di-, or tri-acylation; while the spermidine molecule in AtSDT might adopt a linear conformation, which only allows mono- or di-acylation to take place. In addition, through sequence similarity network (SSN) and structural modeling analysis, we successfully predicted and verified the functions of two uncharacterized Arabidopsis BAHD acyltransferases, OAO95042.1 and NP_190301.2, which use putrescine as the main acyl-acceptor. Our work provides not only an excellent starting point for understanding multisite acylation in BAHD-family enzymes, but also a feasible methodology for predicting possible acyl acceptor specificity of uncharacterized BAHD-family acyltransferases.
		+
		+	Structural and Biochemical Insights Into Two BAHD Acyltransferases (AtSHT and AtSDT) Involved in Phenolamide Biosynthesis.,Wang C, Li J, Ma M, Lin Z, Hu W, Lin W, Zhang P Front Plant Sci. 2021 Jan 13;11:610118. doi: 10.3389/fpls.2020.610118. , eCollection 2020. PMID:33519864<ref>PMID:33519864</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6lpv" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arabidopsis thaliana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Wang CY]]		[[Category: Wang CY]]
	[[Category: Zhang P]]		[[Category: Zhang P]]

OCA at 05:46, 20 January 2021

2021-01-20T05:46:52Z

←Older revision		Revision as of 05:46, 20 January 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6lpv ~~is ON HOLD until Paper Publication~~	+	==structure of Spermidine hydroxycinnamoyl transferases from Arabidopsis thaliana==
-		+	<StructureSection load='6lpv' size='340' side='right'caption='[[6lpv]]' scene=''>
-	~~Authors~~: ~~Wang~~, C.Y., ~~Zhang~~, P.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LPV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LPV FirstGlance]. <br>
-	~~Description: structure of Spermidine hydroxycinnamoyl transferases from Arabidopsis thaliana~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lpv OCA], [http://pdbe.org/6lpv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lpv RCSB], [http://www.ebi.ac.uk/pdbsum/6lpv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lpv ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Wang~~, C.Y~~]]	+	__TOC__
-	[[Category: Zhang, P]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Wang CY]]
		+	[[Category: Zhang P]]

OCA: Protected "6lpv" [edit=sysop:move=sysop]

2020-01-22T13:38:04Z

Protected "6lpv" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:38, 22 January 2020

OCA: New page: '''Unreleased structure''' The entry 6lpv is ON HOLD until Paper Publication Authors: Wang, C.Y., Zhang, P. Description: structure of Spermidine hydroxycinnamoyl transferases from Arab...

2020-01-22T13:38:02Z

New page: '''Unreleased structure''' The entry 6lpv is ON HOLD until Paper Publication Authors: Wang, C.Y., Zhang, P. Description: structure of Spermidine hydroxycinnamoyl transferases from Arab...

New page

'''Unreleased structure'''

The entry 6lpv is ON HOLD until Paper Publication

Authors: Wang, C.Y., Zhang, P.

Description: structure of Spermidine hydroxycinnamoyl transferases from Arabidopsis thaliana
[[Category: Unreleased Structures]]
[[Category: Wang, C.Y]]
[[Category: Zhang, P]]

6lpv - Revision history

OCA at 14:44, 29 November 2023

OCA at 05:46, 20 January 2021

OCA: Protected "6lpv" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6lpv is ON HOLD until Paper Publication Authors: Wang, C.Y., Zhang, P. Description: structure of Spermidine hydroxycinnamoyl transferases from Arab...