6mbh - Revision history

OCA at 06:28, 11 October 2023

2023-10-11T06:28:01Z

←Older revision		Revision as of 06:28, 11 October 2023
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	<StructureSection load='6mbh' size='340' side='right'caption='[[6mbh]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='6mbh' size='340' side='right'caption='[[6mbh]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mbh]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"archangium_violaceum"_kuhlwein_and_reichenbach_1964 "archangium~~ violaceum~~" kuhlwein and reichenbach 1964~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MBH OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6MBH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mbh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Archangium_violaceum Archangium violaceum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MBH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MBH FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>gphF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~83451~~ "~~Archangium violaceum~~" ~~Kuhlwein and Reichenbach 1964~~])</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6mbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mbh OCA], [~~http~~://pdbe.org/6mbh PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6mbh RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6mbh PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6mbh ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mbh OCA], [https://pdbe.org/6mbh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mbh RCSB], [https://www.ebi.ac.uk/pdbsum/6mbh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mbh ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/U6BSB2_9DELT U6BSB2_9DELT]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Archangium violaceum ~~kuhlwein and reichenbach 1964~~]]	+	[[Category: Archangium violaceum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dodge~~, G J~~]]	+	[[Category: Dodge GJ]]
-	[[Category: Smith~~, J L]]~~	+	[[Category: Smith JL]]
-	~~[[Category: Dehydratase]]~~	+
-	~~[[Category: Enoyl-isomerase]]~~	+
-	~~[[Category: Epimerase]]~~	+
-	~~[[Category: Isomerase]]~~	+
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Multifunctional]]~~	+
-	~~[[Category: Natural product]]~~	+
-	~~[[Category: Olefin]]~~	+
-	~~[[Category: Polyketide]]~~	+
-	~~[[Category: Polyketide synthase~~]]	+

OCA at 13:00, 25 December 2019

2019-12-25T13:00:28Z

←Older revision		Revision as of 13:00, 25 December 2019
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	==GphF DH1 P1711L, L1744P variant: An isomerase-inactive variant of GphF DH1==		==GphF DH1 P1711L, L1744P variant: An isomerase-inactive variant of GphF DH1==
-	<StructureSection load='6mbh' size='340' side='right' caption='[[6mbh]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='6mbh' size='340' side='right'caption='[[6mbh]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6mbh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"archangium_violaceum"_kuhlwein_and_reichenbach_1964 "archangium violaceum" kuhlwein and reichenbach 1964]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MBH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MBH FirstGlance]. <br>		<table><tr><td colspan='2'>[[6mbh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"archangium_violaceum"_kuhlwein_and_reichenbach_1964 "archangium violaceum" kuhlwein and reichenbach 1964]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MBH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MBH FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Archangium violaceum kuhlwein and reichenbach 1964]]		[[Category: Archangium violaceum kuhlwein and reichenbach 1964]]
		+	[[Category: Large Structures]]
	[[Category: Dodge, G J]]		[[Category: Dodge, G J]]
	[[Category: Smith, J L]]		[[Category: Smith, J L]]

OCA at 08:57, 3 October 2018

2018-10-03T08:57:52Z

←Older revision		Revision as of 08:57, 3 October 2018
Line 3:		Line 3:
	<StructureSection load='6mbh' size='340' side='right' caption='[[6mbh]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='6mbh' size='340' side='right' caption='[[6mbh]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mbh]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MBH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MBH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mbh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"archangium_violaceum"_kuhlwein_and_reichenbach_1964 "archangium violaceum" kuhlwein and reichenbach 1964]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MBH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MBH FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mbh OCA], [http://pdbe.org/6mbh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mbh RCSB], [http://www.ebi.ac.uk/pdbsum/6mbh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mbh ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gphF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83451 "Archangium violaceum" Kuhlwein and Reichenbach 1964])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mbh OCA], [http://pdbe.org/6mbh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mbh RCSB], [http://www.ebi.ac.uk/pdbsum/6mbh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mbh ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Archangium violaceum kuhlwein and reichenbach 1964]]
	[[Category: Dodge, G J]]		[[Category: Dodge, G J]]
	[[Category: Smith, J L]]		[[Category: Smith, J L]]

OCA at 19:59, 19 September 2018

2018-09-19T19:59:14Z

←Older revision		Revision as of 19:59, 19 September 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6mbh is ~~ON HOLD~~	+	==GphF DH1 P1711L, L1744P variant: An isomerase-inactive variant of GphF DH1==
		+	<StructureSection load='6mbh' size='340' side='right' caption='[[6mbh]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6mbh]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MBH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MBH FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mbh OCA], [http://pdbe.org/6mbh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mbh RCSB], [http://www.ebi.ac.uk/pdbsum/6mbh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mbh ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Polyketide synthases (PKS) are a rich source of natural products of varied chemical composition and biological significance. Here, we report the characterization of an atypical dehydratase (DH) domain from the PKS pathway for gephyronic acid, an inhibitor of eukaryotic protein synthesis. Using a library of synthetic substrate mimics, the reaction course, stereospecificity, and tolerance to non-native substrates of GphF DH1 are probed via LC-MS analysis. Taken together, the studies establish GphF DH1 as a dual-function dehydratase/isomerase that installs an odd-to-even double bond and yields a product consistent with the isobutenyl terminus of gephyronic acid. The studies also reveal an unexpected C2 epimerase function in catalytic turnover with the native substrate. A 1.55-A crystal structure of GphF DH1 guided mutagenesis experiments to elucidate the roles of key amino acids in the multistep DH1 catalysis, identifying critical functions for leucine and tyrosine side chains. The mutagenesis results were applied to add a secondary isomerase functionality to a nonisomerizing DH in the first successful gain-of-function engineering of a PKS DH. Our studies of GphF DH1 catalysis highlight the versatility of the DH active site and adaptation for a specific catalytic outcome with a specific substrate.

-	~~Authors:~~ Dodge, ~~G.J.~~, ~~Smith~~, J.L.	+	Molecular Basis for Olefin Rearrangement in the Gephyronic Acid Polyketide Synthase.,Dodge GJ, Ronnow D, Taylor RE, Smith JL ACS Chem Biol. 2018 Sep 12. doi: 10.1021/acschembio.8b00645. PMID:30179448<ref>PMID:30179448</ref>

-	~~Description: GphF Dehydratase 1~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: Dodge, G.J]]	+	<div class="pdbe-citations 6mbh" style="background-color:#fffaf0;"></div>
-	[[Category: Smith, J.L]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Dodge, G J]]
		+	[[Category: Smith, J L]]
		+	[[Category: Dehydratase]]
		+	[[Category: Enoyl-isomerase]]
		+	[[Category: Epimerase]]
		+	[[Category: Isomerase]]
		+	[[Category: Lyase]]
		+	[[Category: Multifunctional]]
		+	[[Category: Natural product]]
		+	[[Category: Olefin]]
		+	[[Category: Polyketide]]
		+	[[Category: Polyketide synthase]]

OCA: Protected "6mbh" [edit=sysop:move=sysop]

2018-09-05T08:55:23Z

Protected "6mbh" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:55, 5 September 2018

OCA: New page: '''Unreleased structure''' The entry 6mbh is ON HOLD Authors: Dodge, G.J., Smith, J.L. Description: GphF Dehydratase 1 Category: Unreleased Structures Category: Dodge, G.J [[Ca...

2018-09-05T08:55:22Z

New page: '''Unreleased structure''' The entry 6mbh is ON HOLD Authors: Dodge, G.J., Smith, J.L. Description: GphF Dehydratase 1 Category: Unreleased Structures Category: Dodge, G.J [[Ca...

New page

'''Unreleased structure'''

The entry 6mbh is ON HOLD

Authors: Dodge, G.J., Smith, J.L.

Description: GphF Dehydratase 1
[[Category: Unreleased Structures]]
[[Category: Dodge, G.J]]
[[Category: Smith, J.L]]