6mdp - Revision history

OCA at 14:42, 13 March 2024

2024-03-13T14:42:29Z

←Older revision		Revision as of 14:42, 13 March 2024
Line 3:		Line 3:
	<SX load='6mdp' size='340' side='right' viewer='molstar' caption='[[6mdp]], [[Resolution\|resolution]] 3.80Å' scene=''>		<SX load='6mdp' size='340' side='right' viewer='molstar' caption='[[6mdp]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mdp]] is a 7 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Buffalo_rat Buffalo rat~~] and [~~http~~://en.wikipedia.org/wiki/~~Cho_cell_lines Cho cell lines~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MDP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6MDP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mdp]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MDP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MDP FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>~~, ~~<scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.8Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>NSF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~10029 CHO cell lines]), Snap25, Snap ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[http~~:~~//en.wikipedia.org/wiki/Vesicle~~-~~fusing_ATPase Vesicle~~-~~fusing ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.6 3.6.4.6]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mdp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mdp OCA], [https://pdbe.org/6mdp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mdp RCSB], [https://www.ebi.ac.uk/pdbsum/6mdp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mdp ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6mdp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mdp OCA], [~~http~~://pdbe.org/6mdp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6mdp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6mdp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6mdp ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/NSF_CRIGR NSF_CRIGR]] Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling. [[http://www.uniprot.org/uniprot/SNP25_RAT SNP25_RAT]] t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.	+	[https://www.uniprot.org/uniprot/NSF_CRIGR NSF_CRIGR] Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The recycling of SNARE proteins following complex formation and membrane fusion is an essential process in eukaryotic trafficking. A highly conserved AAA+ protein, NSF (N-ethylmaleimide sensitive factor) and an adaptor protein, SNAP (soluble NSF attachment protein), disassembles the SNARE complex. We report electron-cryomicroscopy structures of the complex of NSF, alphaSNAP, and the full-length soluble neuronal SNARE complex (composed of syntaxin-1A, synaptobrevin-2, SNAP-25A) in the presence of ATP under non-hydrolyzing conditions at ~3.9 A resolution. These structures reveal electrostatic interactions by which two alphaSNAP molecules interface with a specific surface of the SNARE complex. This interaction positions the SNAREs such that the 15 N-terminal residues of SNAP-25A are loaded into the D1 ring pore of NSF via a spiral pattern of interactions between a conserved tyrosine NSF residue and SNAP-25A backbone atoms. This loading process likely precedes ATP hydrolysis. Subsequent ATP hydrolysis then drives complete disassembly.	+
-		+
-	Structural principles of SNARE complex recognition by the AAA+ protein NSF.,White KI, Zhao M, Choi UB, Pfuetzner RA, Brunger AT Elife. 2018 Sep 10;7. pii: 38888. doi: 10.7554/eLife.38888. PMID:30198481<ref>PMID:30198481</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6mdp" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[ATPase 3D structures\|ATPase 3D structures]]		*[[ATPase 3D structures\|ATPase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</SX>		</SX>
-	[[Category: ~~Buffalo rat]]~~	+	[[Category: Cricetulus griseus]]
-	~~[[Category: Cho cell lines~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Vesicle-fusing ATPase~~]]	+	[[Category: Rattus norvegicus]]
-	[[Category: Brunger~~, A T~~]]	+	[[Category: Brunger AT]]
-	[[Category: White~~, K I~~]]	+	[[Category: White KI]]
-	[[Category: Zhao, M]]	+	[[Category: Zhao M]]
-	~~[[Category: Aaa]]~~	+
-	~~[[Category: Atpase]]~~	+
-	~~[[Category: Disassembly]]~~	+
-	~~[[Category: Exocytosis]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Membrane fusion]]~~	+
-	~~[[Category: Nsf]]~~	+
-	~~[[Category: Snap]]~~	+
-	~~[[Category: Snare]]~~	+
-	~~[[Category: Synapse~~]]	+

OCA at 02:41, 11 April 2020

2020-04-11T02:41:53Z

←Older revision		Revision as of 02:41, 11 April 2020
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	<SX load='6mdp' size='340' side='right' viewer='molstar' caption='[[6mdp]], [[Resolution\|resolution]] 3.80Å' scene=''>		<SX load='6mdp' size='340' side='right' viewer='molstar' caption='[[6mdp]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mdp]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat] and [http://en.wikipedia.org/wiki/Cho_cell_lines Cho cell lines]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MDP OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6MDP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mdp]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat] and [http://en.wikipedia.org/wiki/Cho_cell_lines Cho cell lines]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MDP OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6MDP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NSF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10029 CHO cell lines]), Snap25, Snap ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NSF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10029 CHO cell lines]), Snap25, Snap ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Vesicle-fusing_ATPase Vesicle-fusing ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.6 3.6.4.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Vesicle-fusing_ATPase Vesicle-fusing ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.6 3.6.4.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6mdp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mdp OCA], [http://pdbe.org/6mdp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mdp RCSB], [http://www.ebi.ac.uk/pdbsum/6mdp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mdp ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6mdp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mdp OCA], [http://pdbe.org/6mdp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mdp RCSB], [http://www.ebi.ac.uk/pdbsum/6mdp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mdp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 22:32, 6 March 2020

2020-03-06T22:32:28Z

←Older revision		Revision as of 22:32, 6 March 2020
Line 1:		Line 1:

	==The D1 and D2 domain rings of NSF engaging the SNAP-25 N-terminus within the 20S supercomplex (focused refinement on D1/D2 rings, class 2)==		==The D1 and D2 domain rings of NSF engaging the SNAP-25 N-terminus within the 20S supercomplex (focused refinement on D1/D2 rings, class 2)==
-	<~~StructureSection~~ load='6mdp' size='340' side='right'caption='[[6mdp]], [[Resolution\|resolution]] 3.80Å' scene=''>	+	<SX load='6mdp' size='340' side='right' viewer='molstar' caption='[[6mdp]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6mdp]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat] and [http://en.wikipedia.org/wiki/Cho_cell_lines Cho cell lines]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MDP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MDP FirstGlance]. <br>		<table><tr><td colspan='2'>[[6mdp]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat] and [http://en.wikipedia.org/wiki/Cho_cell_lines Cho cell lines]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MDP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MDP FirstGlance]. <br>
Line 26:		Line 26:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Buffalo rat]]		[[Category: Buffalo rat]]
	[[Category: Cho cell lines]]		[[Category: Cho cell lines]]

OCA at 10:52, 18 December 2019

2019-12-18T10:52:24Z

←Older revision		Revision as of 10:52, 18 December 2019
Line 1:		Line 1:

	==The D1 and D2 domain rings of NSF engaging the SNAP-25 N-terminus within the 20S supercomplex (focused refinement on D1/D2 rings, class 2)==		==The D1 and D2 domain rings of NSF engaging the SNAP-25 N-terminus within the 20S supercomplex (focused refinement on D1/D2 rings, class 2)==
-	<StructureSection load='6mdp' size='340' side='right' caption='[[6mdp]], [[Resolution\|resolution]] 3.80Å' scene=''>	+	<StructureSection load='6mdp' size='340' side='right'caption='[[6mdp]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6mdp]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat] and [http://en.wikipedia.org/wiki/Cho_cell_lines Cho cell lines]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MDP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MDP FirstGlance]. <br>		<table><tr><td colspan='2'>[[6mdp]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat] and [http://en.wikipedia.org/wiki/Cho_cell_lines Cho cell lines]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MDP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MDP FirstGlance]. <br>
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 6mdp" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6mdp" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[ATPase 3D structures\|ATPase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 29:
	[[Category: Buffalo rat]]		[[Category: Buffalo rat]]
	[[Category: Cho cell lines]]		[[Category: Cho cell lines]]
		+	[[Category: Large Structures]]
	[[Category: Vesicle-fusing ATPase]]		[[Category: Vesicle-fusing ATPase]]
	[[Category: Brunger, A T]]		[[Category: Brunger, A T]]

OCA at 08:45, 26 September 2018

2018-09-26T08:45:25Z

←Older revision		Revision as of 08:45, 26 September 2018
Line 3:		Line 3:
	<StructureSection load='6mdp' size='340' side='right' caption='[[6mdp]], [[Resolution\|resolution]] 3.80Å' scene=''>		<StructureSection load='6mdp' size='340' side='right' caption='[[6mdp]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mdp]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MDP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MDP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mdp]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat] and [http://en.wikipedia.org/wiki/Cho_cell_lines Cho cell lines]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MDP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MDP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NSF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10029 CHO cell lines]), Snap25, Snap ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Vesicle-fusing_ATPase Vesicle-fusing ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.6 3.6.4.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Vesicle-fusing_ATPase Vesicle-fusing ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.6 3.6.4.6] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mdp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mdp OCA], [http://pdbe.org/6mdp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mdp RCSB], [http://www.ebi.ac.uk/pdbsum/6mdp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mdp ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mdp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mdp OCA], [http://pdbe.org/6mdp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mdp RCSB], [http://www.ebi.ac.uk/pdbsum/6mdp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mdp ProSAT]</span></td></tr>
Line 10:		Line 11:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/NSF_CRIGR NSF_CRIGR]] Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling. [[http://www.uniprot.org/uniprot/SNP25_RAT SNP25_RAT]] t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.		[[http://www.uniprot.org/uniprot/NSF_CRIGR NSF_CRIGR]] Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling. [[http://www.uniprot.org/uniprot/SNP25_RAT SNP25_RAT]] t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The recycling of SNARE proteins following complex formation and membrane fusion is an essential process in eukaryotic trafficking. A highly conserved AAA+ protein, NSF (N-ethylmaleimide sensitive factor) and an adaptor protein, SNAP (soluble NSF attachment protein), disassembles the SNARE complex. We report electron-cryomicroscopy structures of the complex of NSF, alphaSNAP, and the full-length soluble neuronal SNARE complex (composed of syntaxin-1A, synaptobrevin-2, SNAP-25A) in the presence of ATP under non-hydrolyzing conditions at ~3.9 A resolution. These structures reveal electrostatic interactions by which two alphaSNAP molecules interface with a specific surface of the SNARE complex. This interaction positions the SNAREs such that the 15 N-terminal residues of SNAP-25A are loaded into the D1 ring pore of NSF via a spiral pattern of interactions between a conserved tyrosine NSF residue and SNAP-25A backbone atoms. This loading process likely precedes ATP hydrolysis. Subsequent ATP hydrolysis then drives complete disassembly.
		+
		+	Structural principles of SNARE complex recognition by the AAA+ protein NSF.,White KI, Zhao M, Choi UB, Pfuetzner RA, Brunger AT Elife. 2018 Sep 10;7. pii: 38888. doi: 10.7554/eLife.38888. PMID:30198481<ref>PMID:30198481</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6mdp" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Buffalo rat]]
		+	[[Category: Cho cell lines]]
	[[Category: Vesicle-fusing ATPase]]		[[Category: Vesicle-fusing ATPase]]
	[[Category: Brunger, A T]]		[[Category: Brunger, A T]]

OCA at 19:59, 19 September 2018

2018-09-19T19:59:58Z

←Older revision		Revision as of 19:59, 19 September 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6mdp is ON HOLD~~	+	==The D1 and D2 domain rings of NSF engaging the SNAP-25 N-terminus within the 20S supercomplex (focused refinement on D1/D2 rings, class 2)==
-		+	<StructureSection load='6mdp' size='340' side='right' caption='[[6mdp]], [[Resolution\|resolution]] 3.80Å' scene=''>
-	~~Authors: White, K.I., Zhao, M., Brunger, A.T.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6mdp]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MDP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MDP FirstGlance]. <br>
-	~~Description:~~ The D1 and D2 domain rings of NSF engaging the SNAP-25 N-terminus within the 20S supercomplex (focused refinement on D1/D2 rings, class 2)	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Vesicle-fusing_ATPase Vesicle-fusing ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.6 3.6.4.6] </span></td></tr>
-	[[Category: White, K.I]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mdp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mdp OCA], [http://pdbe.org/6mdp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mdp RCSB], [http://www.ebi.ac.uk/pdbsum/6mdp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mdp ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NSF_CRIGR NSF_CRIGR]] Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling. [[http://www.uniprot.org/uniprot/SNP25_RAT SNP25_RAT]] t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Vesicle-fusing ATPase]]
		+	[[Category: Brunger, A T]]
		+	[[Category: White, K I]]
	[[Category: Zhao, M]]		[[Category: Zhao, M]]
-	[[Category: ~~Brunger, A.T~~]]	+	[[Category: Aaa]]
		+	[[Category: Atpase]]
		+	[[Category: Disassembly]]
		+	[[Category: Exocytosis]]
		+	[[Category: Hydrolase]]
		+	[[Category: Membrane fusion]]
		+	[[Category: Nsf]]
		+	[[Category: Snap]]
		+	[[Category: Snare]]
		+	[[Category: Synapse]]

OCA: Protected "6mdp" [edit=sysop:move=sysop]

2018-09-12T06:15:52Z

Protected "6mdp" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:15, 12 September 2018

OCA: New page: '''Unreleased structure''' The entry 6mdp is ON HOLD Authors: White, K.I., Zhao, M., Brunger, A.T. Description: The D1 and D2 domain rings of NSF engaging the SNAP-25 N-terminus within...

2018-09-12T06:15:51Z

New page: '''Unreleased structure''' The entry 6mdp is ON HOLD Authors: White, K.I., Zhao, M., Brunger, A.T. Description: The D1 and D2 domain rings of NSF engaging the SNAP-25 N-terminus within...

New page

'''Unreleased structure'''

The entry 6mdp is ON HOLD

Authors: White, K.I., Zhao, M., Brunger, A.T.

Description: The D1 and D2 domain rings of NSF engaging the SNAP-25 N-terminus within the 20S supercomplex (focused refinement on D1/D2 rings, class 2)
[[Category: Unreleased Structures]]
[[Category: White, K.I]]
[[Category: Zhao, M]]
[[Category: Brunger, A.T]]