6mlb - Revision history

OCA at 06:34, 11 October 2023

2023-10-11T06:34:01Z

←Older revision		Revision as of 06:34, 11 October 2023
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	<StructureSection load='6mlb' size='340' side='right'caption='[[6mlb]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='6mlb' size='340' side='right'caption='[[6mlb]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mlb]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MLB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6MLB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mlb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MLB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MLB FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.15Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RBP2, CRBP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6mlb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mlb OCA], [~~http~~://pdbe.org/6mlb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6mlb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6mlb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6mlb ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mlb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mlb OCA], [https://pdbe.org/6mlb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mlb RCSB], [https://www.ebi.ac.uk/pdbsum/6mlb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mlb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/RET2_HUMAN RET2_HUMAN]] Intracellular transport of retinol.	+	[https://www.uniprot.org/uniprot/RET2_HUMAN RET2_HUMAN] Intracellular transport of retinol.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Geiger, J]]	+	[[Category: Geiger J]]
-	[[Category: Ghanbarpour, A]]	+	[[Category: Ghanbarpour A]]
-	~~[[Category: Ilbp]]~~	+
-	~~[[Category: Lipid binding protein]]~~	+
-	~~[[Category: Protein switch~~]]	+

OCA at 10:59, 18 December 2019

2019-12-18T10:59:37Z

←Older revision		Revision as of 10:59, 18 December 2019
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	</div>		</div>
	<div class="pdbe-citations 6mlb" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6mlb" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Retinol-binding protein 3D structures\|Retinol-binding protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:39, 13 November 2019

2019-11-13T11:39:09Z

←Older revision		Revision as of 11:39, 13 November 2019
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	<StructureSection load='6mlb' size='340' side='right'caption='[[6mlb]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='6mlb' size='340' side='right'caption='[[6mlb]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mlb]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MLB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MLB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mlb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MLB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MLB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RBP2, CRBP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mlb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mlb OCA], [http://pdbe.org/6mlb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mlb RCSB], [http://www.ebi.ac.uk/pdbsum/6mlb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mlb ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mlb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mlb OCA], [http://pdbe.org/6mlb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mlb RCSB], [http://www.ebi.ac.uk/pdbsum/6mlb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mlb ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Geiger, J]]		[[Category: Geiger, J]]

OCA at 05:55, 16 October 2019

2019-10-16T05:55:14Z

←Older revision		Revision as of 05:55, 16 October 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6mlb is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of the holo retinal-bound domain-swapped dimer Q108K:K40L:T51F mutant of human cellular retinol binding protein II==
		+	<StructureSection load='6mlb' size='340' side='right'caption='[[6mlb]], [[Resolution\|resolution]] 2.15Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6mlb]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MLB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MLB FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mlb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mlb OCA], [http://pdbe.org/6mlb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mlb RCSB], [http://www.ebi.ac.uk/pdbsum/6mlb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mlb ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RET2_HUMAN RET2_HUMAN]] Intracellular transport of retinol.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Protein conformational switches or allosteric proteins play a key role in the regulation of many essential biological pathways. Nonetheless, the implementation of protein conformational switches in protein design applications has proven challenging, with only a few known examples that are not derivatives of naturally occurring allosteric systems. We have discovered that the domain swapped (DS) dimer of hCRBPII undergoes a large and robust conformational change upon retinal binding, making it a potentially powerful template for the design of protein conformational switches. Atomic resolution structures of the apo- and holo- forms illuminate a simple, mechanical mechanism involving sterically driven torsion angle flipping of two residues that drive the motion. We further demonstrate that the con-formational "readout" can be altered by addition of cross-domain disulfide bonds, also visualized at atomic resolution. Finally, as a proof of principle, we have created an allosteric metal binding site in the DS dimer, where ligand binding results in a reversible five-fold loss of metal binding affinity. The high resolution structure of the metal-bound variant illustrates a well-formed metal binding site at the inter-face of the two domains of the DS dimer, and confirms the design strategy for allosteric regulation.

-	~~Authors: Ghanbarpour~~, A., ~~Geiger~~, J.	+	Engineering the hCRBPII domain-swapped dimer into a new class of protein switches.,Ghanbarpour A, Pinger C, Esmatpour Salmani R, Assar Z, Santos EM, Nosrati M, Pawlowski K, Spence D, Vasileiou C, Jin X, Borhan B, Geiger JH J Am Chem Soc. 2019 Sep 26. doi: 10.1021/jacs.9b04664. PMID:31557439<ref>PMID:31557439</ref>

-	~~Description: Crystal structure~~ of the ~~holo retinal~~-~~bound domain~~-~~swapped dimer Q108K~~:~~K40L:T51F mutant of human cellular retinol binding protein II~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	~~[[Category: Ghanbarpour, A~~]]	+	<div class="pdbe-citations 6mlb" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Geiger, J]]		[[Category: Geiger, J]]
		+	[[Category: Ghanbarpour, A]]
		+	[[Category: Ilbp]]
		+	[[Category: Lipid binding protein]]
		+	[[Category: Protein switch]]

OCA at 04:13, 7 August 2019

2019-08-07T04:13:27Z

←Older revision		Revision as of 04:13, 7 August 2019
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	Authors: Ghanbarpour, A., Geiger, J.		Authors: Ghanbarpour, A., Geiger, J.

-	Description: Crystal structure of the holo retinal-bound domain-swapped dimer Q108K:K40L:T51F mutant of human ~~Cellular Retinol Binding Protein~~ II	+	Description: Crystal structure of the holo retinal-bound domain-swapped dimer Q108K:K40L:T51F mutant of human cellular retinol binding protein II
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Ghanbarpour, A]]		[[Category: Ghanbarpour, A]]
	[[Category: Geiger, J]]		[[Category: Geiger, J]]

OCA at 06:32, 17 October 2018

2018-10-17T06:32:34Z

←Older revision		Revision as of 06:32, 17 October 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6mlb is ON HOLD	+	The entry 6mlb is ON HOLD until Paper Publication

	Authors: Ghanbarpour, A., Geiger, J.		Authors: Ghanbarpour, A., Geiger, J.

OCA: Protected "6mlb" [edit=sysop:move=sysop]

2018-10-03T06:31:12Z

Protected "6mlb" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:31, 3 October 2018

OCA: New page: '''Unreleased structure''' The entry 6mlb is ON HOLD Authors: Ghanbarpour, A., Geiger, J. Description: Crystal structure of the holo retinal-bound domain-swapped dimer Q108K:K40L:T51F ...

2018-10-03T06:31:11Z

New page: '''Unreleased structure''' The entry 6mlb is ON HOLD Authors: Ghanbarpour, A., Geiger, J. Description: Crystal structure of the holo retinal-bound domain-swapped dimer Q108K:K40L:T51F ...

New page

'''Unreleased structure'''

The entry 6mlb is ON HOLD

Authors: Ghanbarpour, A., Geiger, J.

Description: Crystal structure of the holo retinal-bound domain-swapped dimer Q108K:K40L:T51F mutant of human Cellular Retinol Binding Protein II
[[Category: Unreleased Structures]]
[[Category: Ghanbarpour, A]]
[[Category: Geiger, J]]