6mqu - Revision history

OCA at 14:44, 13 March 2024

2024-03-13T14:44:27Z

←Older revision		Revision as of 14:44, 13 March 2024
Line 3:		Line 3:
	<StructureSection load='6mqu' size='340' side='right'caption='[[6mqu]], [[Resolution\|resolution]] 3.17Å' scene=''>		<StructureSection load='6mqu' size='340' side='right'caption='[[6mqu]], [[Resolution\|resolution]] 3.17Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mqu]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MQU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6MQU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mqu]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MQU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MQU FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non~~-~~Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=DAR:D-ARGININE'>DAR</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.17Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6mqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mqu OCA], [~~http~~://pdbe.org/6mqu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6mqu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6mqu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6mqu ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DAR:D-ARGININE'>DAR</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mqu OCA], [https://pdbe.org/6mqu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mqu RCSB], [https://www.ebi.ac.uk/pdbsum/6mqu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mqu ProSAT]</span></td></tr>
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The features that stabilize the structures of membrane proteins remain poorly understood. Polar interactions contribute modestly, and the hydrophobic effect contributes little to the energetics of apolar side-chain packing in membranes. Disruption of steric packing can destabilize the native folds of membrane proteins, but is packing alone sufficient to drive folding in lipids? If so, then membrane proteins stabilized by this feature should be readily designed and structurally characterized-yet this has not been achieved. Through simulation of the natural protein phospholamban and redesign of variants, we define a steric packing code underlying its assembly. Synthetic membrane proteins designed using this code and stabilized entirely by apolar side chains conform to the intended fold. Although highly stable, the steric complementarity required for their folding is surprisingly stringent. Structural informatics shows that the designed packing motif recurs across the proteome, emphasizing a prominent role for precise apolar packing in membrane protein folding, stabilization, and evolution.

-	Packing of apolar side chains enables accurate design of highly stable membrane proteins.,Mravic M, Thomaston JL, Tucker M, Solomon PE, Liu L, DeGrado WF Science. 2019 Mar 29;363(6434):1418-1423. doi: 10.1126/science.aav7541. PMID:30923216<ref>PMID:30923216</ref>	+	==See Also==
-		+	*[[Phospholamban\|Phospholamban]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6mqu" style="background-color:#fffaf0;"></div>~~	+
-	== ~~References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~DeGrado, W F~~]]	+	[[Category: Synthetic construct]]
-	[[Category: ~~Mravic, M~~]]	+	[[Category: DeGrado WF]]
-	[[Category: ~~Thomaston, J L~~]]	+	[[Category: Mravic M]]
-	[[Category: ~~De novo protein]]~~	+	[[Category: Thomaston JL]]
-	~~[[Category: Designed]]~~	+
-	~~[[Category: Engineered]]~~	+
-	~~[[Category: Pentamer]]~~	+
-	~~[[Category: Phospholamban]]~~	+
-	~~[[Category: Transmembrane~~]]	+

OCA at 07:57, 10 April 2019

2019-04-10T07:57:46Z

←Older revision		Revision as of 07:57, 10 April 2019
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mqu OCA], [http://pdbe.org/6mqu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mqu RCSB], [http://www.ebi.ac.uk/pdbsum/6mqu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mqu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mqu OCA], [http://pdbe.org/6mqu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mqu RCSB], [http://www.ebi.ac.uk/pdbsum/6mqu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mqu ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The features that stabilize the structures of membrane proteins remain poorly understood. Polar interactions contribute modestly, and the hydrophobic effect contributes little to the energetics of apolar side-chain packing in membranes. Disruption of steric packing can destabilize the native folds of membrane proteins, but is packing alone sufficient to drive folding in lipids? If so, then membrane proteins stabilized by this feature should be readily designed and structurally characterized-yet this has not been achieved. Through simulation of the natural protein phospholamban and redesign of variants, we define a steric packing code underlying its assembly. Synthetic membrane proteins designed using this code and stabilized entirely by apolar side chains conform to the intended fold. Although highly stable, the steric complementarity required for their folding is surprisingly stringent. Structural informatics shows that the designed packing motif recurs across the proteome, emphasizing a prominent role for precise apolar packing in membrane protein folding, stabilization, and evolution.
		+
		+	Packing of apolar side chains enables accurate design of highly stable membrane proteins.,Mravic M, Thomaston JL, Tucker M, Solomon PE, Liu L, DeGrado WF Science. 2019 Mar 29;363(6434):1418-1423. doi: 10.1126/science.aav7541. PMID:30923216<ref>PMID:30923216</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6mqu" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:07, 3 April 2019

2019-04-03T07:07:29Z

←Older revision		Revision as of 07:07, 3 April 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6mqu ~~is ON HOLD until Paper Publication~~	+	==PL5, synthetic transmembrane domain variant of human phospholamban==
-		+	<StructureSection load='6mqu' size='340' side='right'caption='[[6mqu]], [[Resolution\|resolution]] 3.17Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6mqu]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MQU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MQU FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DAR:D-ARGININE'>DAR</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mqu OCA], [http://pdbe.org/6mqu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mqu RCSB], [http://www.ebi.ac.uk/pdbsum/6mqu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mqu ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: DeGrado, W F]]
		+	[[Category: Mravic, M]]
		+	[[Category: Thomaston, J L]]
		+	[[Category: De novo protein]]
		+	[[Category: Designed]]
		+	[[Category: Engineered]]
		+	[[Category: Pentamer]]
		+	[[Category: Phospholamban]]
		+	[[Category: Transmembrane]]

OCA: Protected "6mqu" [edit=sysop:move=sysop]

2018-10-17T06:40:22Z

Protected "6mqu" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:40, 17 October 2018

OCA: New page: '''Unreleased structure''' The entry 6mqu is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

2018-10-17T06:40:21Z

New page: '''Unreleased structure''' The entry 6mqu is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6mqu is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]