6mtg - Revision history

OCA at 06:38, 11 October 2023

2023-10-11T06:38:27Z

←Older revision		Revision as of 06:38, 11 October 2023
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	<StructureSection load='6mtg' size='340' side='right'caption='[[6mtg]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='6mtg' size='340' side='right'caption='[[6mtg]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mtg]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MTG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6MTG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mtg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MTG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MTG FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=FMT:FORMIC+ACID'>FMT</scene~~>, ~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.85Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">metAS~~, ~~metA, b4013, JW3973 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])~~<~~/td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mtg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mtg OCA], [https://pdbe.org/6mtg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mtg RCSB], [https://www.ebi.ac.uk/pdbsum/6mtg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mtg ProSAT]</span></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/Homoserine_O-succinyltransferase Homoserine O-succinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.46 2.3.1.46]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6mtg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mtg OCA], [~~http~~://pdbe.org/6mtg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6mtg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6mtg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6mtg ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/METAS_ECOLI METAS_ECOLI]] Transfers a succinyl group from succinyl-CoA to L-homoserine, forming succinyl-L-homoserine (PubMed:10572016, PubMed:17442255, PubMed:17302437, PubMed:28581482). Utilizes a ping-pong kinetic mechanism in which the succinyl group of succinyl-CoA is initially transferred to the enzyme to form a succinyl-enzyme intermediate before subsequent transfer to homoserine to form the final product, O-succinylhomoserine (PubMed:10572016, PubMed:17442255, PubMed:17302437). Cannot use acetyl-CoA (PubMed:10572016).<ref>PMID:10572016</ref> <ref>PMID:17302437</ref> <ref>PMID:17442255</ref> <ref>PMID:28581482</ref>	+	[https://www.uniprot.org/uniprot/METAS_ECOLI METAS_ECOLI] Transfers a succinyl group from succinyl-CoA to L-homoserine, forming succinyl-L-homoserine (PubMed:10572016, PubMed:17442255, PubMed:17302437, PubMed:28581482). Utilizes a ping-pong kinetic mechanism in which the succinyl group of succinyl-CoA is initially transferred to the enzyme to form a succinyl-enzyme intermediate before subsequent transfer to homoserine to form the final product, O-succinylhomoserine (PubMed:10572016, PubMed:17442255, PubMed:17302437). Cannot use acetyl-CoA (PubMed:10572016).<ref>PMID:10572016</ref> <ref>PMID:17302437</ref> <ref>PMID:17442255</ref> <ref>PMID:28581482</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli]]~~	+	[[Category: Escherichia coli K-12]]
-	~~[[Category: Homoserine O~~-~~succinyltransferase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Han~~, G W~~]]	+	[[Category: Han GW]]
-	[[Category: Li~~, J C~~]]	+	[[Category: Li JC]]
-	[[Category: Nasertorabi, F]]	+	[[Category: Nasertorabi F]]
-	[[Category: Schultz~~, P G~~]]	+	[[Category: Schultz PG]]
-	[[Category: Stevens~~, R C~~]]	+	[[Category: Stevens RC]]
-	[[Category: Xuan, W]]	+	[[Category: Xuan W]]
-	~~[[Category: Crosslink]]~~	+
-	~~[[Category: Isothiocyanate]]~~	+
-	~~[[Category: Noncanonical amino acid]]~~	+
-	~~[[Category: Stabilization]]~~	+
-	~~[[Category: Thiourea]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 06:27, 10 July 2019

2019-07-10T06:27:30Z

←Older revision		Revision as of 06:27, 10 July 2019
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	<StructureSection load='6mtg' size='340' side='right'caption='[[6mtg]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='6mtg' size='340' side='right'caption='[[6mtg]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mtg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MTG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MTG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mtg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MTG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MTG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">metAS, metA, b4013, JW3973 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Homoserine_O-succinyltransferase Homoserine O-succinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.46 2.3.1.46] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Homoserine_O-succinyltransferase Homoserine O-succinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.46 2.3.1.46] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mtg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mtg OCA], [http://pdbe.org/6mtg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mtg RCSB], [http://www.ebi.ac.uk/pdbsum/6mtg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mtg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mtg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mtg OCA], [http://pdbe.org/6mtg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mtg RCSB], [http://www.ebi.ac.uk/pdbsum/6mtg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mtg ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ecoli]]
	[[Category: Homoserine O-succinyltransferase]]		[[Category: Homoserine O-succinyltransferase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 06:55, 26 June 2019

2019-06-26T06:55:11Z

←Older revision		Revision as of 06:55, 26 June 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6mtg is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==A Single Reactive Noncanonical Amino Acid is Able to Dramatically Stabilize Protein Structure==
		+	<StructureSection load='6mtg' size='340' side='right'caption='[[6mtg]], [[Resolution\|resolution]] 1.85Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6mtg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MTG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MTG FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Homoserine_O-succinyltransferase Homoserine O-succinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.46 2.3.1.46] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mtg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mtg OCA], [http://pdbe.org/6mtg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mtg RCSB], [http://www.ebi.ac.uk/pdbsum/6mtg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mtg ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/METAS_ECOLI METAS_ECOLI]] Transfers a succinyl group from succinyl-CoA to L-homoserine, forming succinyl-L-homoserine (PubMed:10572016, PubMed:17442255, PubMed:17302437, PubMed:28581482). Utilizes a ping-pong kinetic mechanism in which the succinyl group of succinyl-CoA is initially transferred to the enzyme to form a succinyl-enzyme intermediate before subsequent transfer to homoserine to form the final product, O-succinylhomoserine (PubMed:10572016, PubMed:17442255, PubMed:17302437). Cannot use acetyl-CoA (PubMed:10572016).<ref>PMID:10572016</ref> <ref>PMID:17302437</ref> <ref>PMID:17442255</ref> <ref>PMID:28581482</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A p-isothiocyanate phenylalanine mutant of the homodimeric protein homoserine o-succinyltransferase (MetA) was isolated in a temperature dependent selection from a library of metA mutants containing noncanonical amino acids. This mutant protein has a dramatic increase of 24 degrees C in thermal stability compared to the wild type protein. Peptide mapping experiments revealed that the isothiocyanate group forms a thiourea cross-link to the N terminal proline of the other monomer, despite the two positions being >30 A apart in the X-ray crystal structure of the wild type protein. These results show that an expanded set of building blocks beyond the canonical 20 amino acids can lead to significant changes in the properties of proteins.

-	~~Authors~~:	+	A Single Reactive Noncanonical Amino Acid Is Able to Dramatically Stabilize Protein Structure.,Li JC, Nastertorabi F, Xuan W, Han GW, Stevens RC, Schultz PG ACS Chem Biol. 2019 Jun 4. doi: 10.1021/acschembio.9b00002. PMID:31181898<ref>PMID:31181898</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6mtg" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homoserine O-succinyltransferase]]
		+	[[Category: Large Structures]]
		+	[[Category: Han, G W]]
		+	[[Category: Li, J C]]
		+	[[Category: Nasertorabi, F]]
		+	[[Category: Schultz, P G]]
		+	[[Category: Stevens, R C]]
		+	[[Category: Xuan, W]]
		+	[[Category: Crosslink]]
		+	[[Category: Isothiocyanate]]
		+	[[Category: Noncanonical amino acid]]
		+	[[Category: Stabilization]]
		+	[[Category: Thiourea]]
		+	[[Category: Transferase]]

OCA at 06:36, 14 November 2018

2018-11-14T06:36:23Z

←Older revision		Revision as of 06:36, 14 November 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6mtg is ON HOLD	+	The entry 6mtg is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6mtg" [edit=sysop:move=sysop]

2018-10-31T05:30:53Z

Protected "6mtg" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:30, 31 October 2018

OCA: New page: '''Unreleased structure''' The entry 6mtg is ON HOLD Authors: Description: Category: Unreleased Structures

2018-10-31T05:30:52Z

New page: '''Unreleased structure''' The entry 6mtg is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6mtg is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]