6mux - Revision history

OCA at 14:45, 13 March 2024

2024-03-13T14:45:15Z

←Older revision		Revision as of 14:45, 13 March 2024
Line 3:		Line 3:
	<SX load='6mux' size='340' side='right' viewer='molstar' caption='[[6mux]], [[Resolution\|resolution]] 3.90Å' scene=''>		<SX load='6mux' size='340' side='right' viewer='molstar' caption='[[6mux]], [[Resolution\|resolution]] 3.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mux]] is a 35 chain structure with sequence from [~~http://en.wikipedia.org/wiki/Plaf7 Plaf7] and [http~~://en.wikipedia.org/wiki/~~Plasmodium_falciparum_3d7~~ Plasmodium falciparum ~~3d7~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MUX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6MUX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mux]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MUX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MUX FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[6dfk\|6dfk]], [[6muw\|6muw]], [[6muv\|6muv]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.9Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">PF3D7_0907700 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=36329 PLAF7])</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mux OCA], [https://pdbe.org/6mux PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mux RCSB], [https://www.ebi.ac.uk/pdbsum/6mux PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mux ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Proteasome_endopeptidase_complex Proteasome endopeptidase complex], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.25.1 3.4.25.1] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6mux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mux OCA], [~~http~~://pdbe.org/6mux PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6mux RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6mux PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6mux ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~Q8IKC9_PLAF7 Q8IKC9_PLAF7~~]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.[RuleBase:RU004203] [[http://www.uniprot.org/uniprot/Q8IDG3_PLAF7 Q8IDG3_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.[RuleBase:RU000551] [[http://www.uniprot.org/uniprot/Q8I6T3_PLAF7 Q8I6T3_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.[RuleBase:RU004203] [[http://www.uniprot.org/uniprot/Q8IJT1_PLAF7 Q8IJT1_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.[RuleBase:RU004203] [[http://www.uniprot.org/uniprot/Q7K6A9_PLAF7 Q7K6A9_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.[RuleBase:RU004203] [[http://www.uniprot.org/uniprot/Q8IDG2_PLAF7 Q8IDG2_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.[RuleBase:RU000551] [[http://www.uniprot.org/uniprot/C0H4E8_PLAF7 C0H4E8_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.[RuleBase:RU004203] [[http://www.uniprot.org/uniprot/Q8IBI3_PLAF7 Q8IBI3_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.[RuleBase:RU000551]	+	[https://www.uniprot.org/uniprot/Q8IAR3_PLAF7 Q8IAR3_PLAF7]
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The activity of the proteasome 20S catalytic core is regulated by protein complexes that bind to one or both ends. The PA28 regulator stimulates 20S proteasome peptidase activity in vitro, but its role in vivo remains unclear. Here, we show that genetic deletion of the PA28 regulator from Plasmodium falciparum (Pf) renders malaria parasites more sensitive to the antimalarial drug dihydroartemisinin, indicating that PA28 may play a role in protection against proteotoxic stress. The crystal structure of PfPA28 reveals a bell-shaped molecule with an inner pore that has a strong segregation of charges. Small-angle X-ray scattering shows that disordered loops, which are not resolved in the crystal structure, extend from the PfPA28 heptamer and surround the pore. Using single particle cryo-electron microscopy, we solved the structure of Pf20S in complex with one and two regulatory PfPA28 caps at resolutions of 3.9 and 3.8 A, respectively. PfPA28 binds Pf20S asymmetrically, strongly engaging subunits on only one side of the core. PfPA28 undergoes rigid body motions relative to Pf20S. Molecular dynamics simulations support conformational flexibility and a leaky interface. We propose lateral transfer of short peptides through the dynamic interface as a mechanism facilitating the release of proteasome degradation products.	+
-		+
-	The structure of the PA28-20S proteasome complex from Plasmodium falciparum and implications for proteostasis.,Xie SC, Metcalfe RD, Hanssen E, Yang T, Gillett DL, Leis AP, Morton CJ, Kuiper MJ, Parker MW, Spillman NJ, Wong W, Tsu C, Dick LR, Griffin MDW, Tilley L Nat Microbiol. 2019 Aug 5. pii: 10.1038/s41564-019-0524-4. doi:, 10.1038/s41564-019-0524-4. PMID:31384003<ref>PMID:31384003</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6mux" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Proteasome 3D structures\|Proteasome 3D structures]]		*[[Proteasome 3D structures\|Proteasome 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</SX>		</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Plaf7]]~~	+	[[Category: Plasmodium falciparum 3D7]]
-	[[Category: Plasmodium falciparum ~~3d7]]~~	+	[[Category: Gillett DL]]
-	~~[[Category: Proteasome endopeptidase complex~~]]	+	[[Category: Griffin MDW]]
-	[[Category: Gillett~~, D L~~]]	+	[[Category: Hanssen E]]
-	[[Category: Griffin~~, M D.W~~]]	+	[[Category: Leis AP]]
-	[[Category: Hanssen, E]]	+	[[Category: Metcalfe RD]]
-	[[Category: Leis~~, A P~~]]	+	[[Category: Tilley L]]
-	[[Category: Metcalfe~~, R D~~]]	+	[[Category: Xie SC]]
-	[[Category: Tilley, L]]	+
-	[[Category: Xie~~, S C]]~~	+
-	~~[[Category: 11s subunit]]~~	+
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Hydrolyse activator]]~~	+
-	~~[[Category: Protease]]~~	+
-	~~[[Category: Proteasome]]~~	+
-	~~[[Category: Proteasome activator~~]]	+

OCA at 02:58, 11 April 2020

2020-04-11T02:58:08Z

←Older revision		Revision as of 02:58, 11 April 2020
Line 3:		Line 3:
	<SX load='6mux' size='340' side='right' viewer='molstar' caption='[[6mux]], [[Resolution\|resolution]] 3.90Å' scene=''>		<SX load='6mux' size='340' side='right' viewer='molstar' caption='[[6mux]], [[Resolution\|resolution]] 3.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mux]] is a 35 chain structure with sequence from [http://en.wikipedia.org/wiki/Plaf7 Plaf7] and [http://en.wikipedia.org/wiki/Plasmodium_falciparum_3d7 Plasmodium falciparum 3d7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MUX OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6MUX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mux]] is a 35 chain structure with sequence from [http://en.wikipedia.org/wiki/Plaf7 Plaf7] and [http://en.wikipedia.org/wiki/Plasmodium_falciparum_3d7 Plasmodium falciparum 3d7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MUX OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6MUX FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6dfk\|6dfk]], [[6muw\|6muw]], [[6muv\|6muv]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6dfk\|6dfk]], [[6muw\|6muw]], [[6muv\|6muv]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PF3D7_0907700 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36329 PLAF7])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PF3D7_0907700 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36329 PLAF7])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Proteasome_endopeptidase_complex Proteasome endopeptidase complex], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.25.1 3.4.25.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Proteasome_endopeptidase_complex Proteasome endopeptidase complex], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.25.1 3.4.25.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6mux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mux OCA], [http://pdbe.org/6mux PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mux RCSB], [http://www.ebi.ac.uk/pdbsum/6mux PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mux ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6mux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mux OCA], [http://pdbe.org/6mux PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mux RCSB], [http://www.ebi.ac.uk/pdbsum/6mux PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mux ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 22:39, 6 March 2020

2020-03-06T22:39:05Z

←Older revision		Revision as of 22:39, 6 March 2020
Line 1:		Line 1:

	==The structure of the Plasmodium falciparum 20S proteasome in complex with one PA28 activator==		==The structure of the Plasmodium falciparum 20S proteasome in complex with one PA28 activator==
-	<~~StructureSection~~ load='6mux' size='340' side='right'caption='[[6mux]], [[Resolution\|resolution]] 3.90Å' scene=''>	+	<SX load='6mux' size='340' side='right' viewer='molstar' caption='[[6mux]], [[Resolution\|resolution]] 3.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6mux]] is a 35 chain structure with sequence from [http://en.wikipedia.org/wiki/Plaf7 Plaf7] and [http://en.wikipedia.org/wiki/Plasmodium_falciparum_3d7 Plasmodium falciparum 3d7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MUX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MUX FirstGlance]. <br>		<table><tr><td colspan='2'>[[6mux]] is a 35 chain structure with sequence from [http://en.wikipedia.org/wiki/Plaf7 Plaf7] and [http://en.wikipedia.org/wiki/Plasmodium_falciparum_3d7 Plasmodium falciparum 3d7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MUX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MUX FirstGlance]. <br>
Line 26:		Line 26:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Plaf7]]		[[Category: Plaf7]]

OCA at 11:12, 18 December 2019

2019-12-18T11:12:32Z

←Older revision		Revision as of 11:12, 18 December 2019
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 6mux" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6mux" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Proteasome 3D structures\|Proteasome 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:25, 14 August 2019

2019-08-14T17:25:14Z

←Older revision		Revision as of 17:25, 14 August 2019
Line 3:		Line 3:
	<StructureSection load='6mux' size='340' side='right'caption='[[6mux]], [[Resolution\|resolution]] 3.90Å' scene=''>		<StructureSection load='6mux' size='340' side='right'caption='[[6mux]], [[Resolution\|resolution]] 3.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mux]] is a 35 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Plasmodium_falciparum_3d7 Plasmodium falciparum 3d7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MUX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MUX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mux]] is a 35 chain structure with sequence from [http://en.wikipedia.org/wiki/Plaf7 Plaf7] and [http://en.wikipedia.org/wiki/Plasmodium_falciparum_3d7 Plasmodium falciparum 3d7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MUX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MUX FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6dfk\|6dfk]], [[6muw\|6muw]], [[6muv\|6muv]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6dfk\|6dfk]], [[6muw\|6muw]], [[6muv\|6muv]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PF3D7_0907700 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36329 PLAF7])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Proteasome_endopeptidase_complex Proteasome endopeptidase complex], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.25.1 3.4.25.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Proteasome_endopeptidase_complex Proteasome endopeptidase complex], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.25.1 3.4.25.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mux OCA], [http://pdbe.org/6mux PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mux RCSB], [http://www.ebi.ac.uk/pdbsum/6mux PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mux ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mux OCA], [http://pdbe.org/6mux PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mux RCSB], [http://www.ebi.ac.uk/pdbsum/6mux PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mux ProSAT]</span></td></tr>
Line 10:		Line 11:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/Q8IKC9_PLAF7 Q8IKC9_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.[RuleBase:RU004203] [[http://www.uniprot.org/uniprot/Q8IDG3_PLAF7 Q8IDG3_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.[RuleBase:RU000551] [[http://www.uniprot.org/uniprot/Q8I6T3_PLAF7 Q8I6T3_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.[RuleBase:RU004203] [[http://www.uniprot.org/uniprot/Q8IJT1_PLAF7 Q8IJT1_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.[RuleBase:RU004203] [[http://www.uniprot.org/uniprot/Q7K6A9_PLAF7 Q7K6A9_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.[RuleBase:RU004203] [[http://www.uniprot.org/uniprot/Q8IDG2_PLAF7 Q8IDG2_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.[RuleBase:RU000551] [[http://www.uniprot.org/uniprot/C0H4E8_PLAF7 C0H4E8_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.[RuleBase:RU004203] [[http://www.uniprot.org/uniprot/Q8IBI3_PLAF7 Q8IBI3_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.[RuleBase:RU000551]		[[http://www.uniprot.org/uniprot/Q8IKC9_PLAF7 Q8IKC9_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.[RuleBase:RU004203] [[http://www.uniprot.org/uniprot/Q8IDG3_PLAF7 Q8IDG3_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.[RuleBase:RU000551] [[http://www.uniprot.org/uniprot/Q8I6T3_PLAF7 Q8I6T3_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.[RuleBase:RU004203] [[http://www.uniprot.org/uniprot/Q8IJT1_PLAF7 Q8IJT1_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.[RuleBase:RU004203] [[http://www.uniprot.org/uniprot/Q7K6A9_PLAF7 Q7K6A9_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.[RuleBase:RU004203] [[http://www.uniprot.org/uniprot/Q8IDG2_PLAF7 Q8IDG2_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.[RuleBase:RU000551] [[http://www.uniprot.org/uniprot/C0H4E8_PLAF7 C0H4E8_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.[RuleBase:RU004203] [[http://www.uniprot.org/uniprot/Q8IBI3_PLAF7 Q8IBI3_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.[RuleBase:RU000551]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The activity of the proteasome 20S catalytic core is regulated by protein complexes that bind to one or both ends. The PA28 regulator stimulates 20S proteasome peptidase activity in vitro, but its role in vivo remains unclear. Here, we show that genetic deletion of the PA28 regulator from Plasmodium falciparum (Pf) renders malaria parasites more sensitive to the antimalarial drug dihydroartemisinin, indicating that PA28 may play a role in protection against proteotoxic stress. The crystal structure of PfPA28 reveals a bell-shaped molecule with an inner pore that has a strong segregation of charges. Small-angle X-ray scattering shows that disordered loops, which are not resolved in the crystal structure, extend from the PfPA28 heptamer and surround the pore. Using single particle cryo-electron microscopy, we solved the structure of Pf20S in complex with one and two regulatory PfPA28 caps at resolutions of 3.9 and 3.8 A, respectively. PfPA28 binds Pf20S asymmetrically, strongly engaging subunits on only one side of the core. PfPA28 undergoes rigid body motions relative to Pf20S. Molecular dynamics simulations support conformational flexibility and a leaky interface. We propose lateral transfer of short peptides through the dynamic interface as a mechanism facilitating the release of proteasome degradation products.
		+
		+	The structure of the PA28-20S proteasome complex from Plasmodium falciparum and implications for proteostasis.,Xie SC, Metcalfe RD, Hanssen E, Yang T, Gillett DL, Leis AP, Morton CJ, Kuiper MJ, Parker MW, Spillman NJ, Wong W, Tsu C, Dick LR, Griffin MDW, Tilley L Nat Microbiol. 2019 Aug 5. pii: 10.1038/s41564-019-0524-4. doi:, 10.1038/s41564-019-0524-4. PMID:31384003<ref>PMID:31384003</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6mux" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Plaf7]]
	[[Category: Plasmodium falciparum 3d7]]		[[Category: Plasmodium falciparum 3d7]]
	[[Category: Proteasome endopeptidase complex]]		[[Category: Proteasome endopeptidase complex]]

OCA at 05:55, 7 August 2019

2019-08-07T05:55:02Z

←Older revision		Revision as of 05:55, 7 August 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The ~~entry~~ 6mux is ~~ON HOLD until Paper Publication~~	+	==The structure of the Plasmodium falciparum 20S proteasome in complex with one PA28 activator==
-		+	<StructureSection load='6mux' size='340' side='right'caption='[[6mux]], [[Resolution\|resolution]] 3.90Å' scene=''>
-	~~Authors~~: ~~Metcalfe~~, R.D., ~~Xie~~, S.C., ~~Hanssen~~, E., ~~Gillett~~, D.L., ~~Leis~~, A.P., ~~Tilley~~, L., ~~Griffin~~, M.D.W.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6mux]] is a 35 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Plasmodium_falciparum_3d7 Plasmodium falciparum 3d7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MUX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MUX FirstGlance]. <br>
-	~~Description~~: The ~~structure of~~ the ~~Plasmodium falciparum 20S~~ proteasome in complex with ~~one PA28 activator~~	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6dfk\|6dfk]], [[6muw\|6muw]], [[6muv\|6muv]]</td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Proteasome_endopeptidase_complex Proteasome endopeptidase complex], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.25.1 3.4.25.1] </span></td></tr>
-	[[Category: Metcalfe, R.D]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mux OCA], [http://pdbe.org/6mux PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mux RCSB], [http://www.ebi.ac.uk/pdbsum/6mux PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mux ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q8IKC9_PLAF7 Q8IKC9_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.[RuleBase:RU004203] [[http://www.uniprot.org/uniprot/Q8IDG3_PLAF7 Q8IDG3_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.[RuleBase:RU000551] [[http://www.uniprot.org/uniprot/Q8I6T3_PLAF7 Q8I6T3_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.[RuleBase:RU004203] [[http://www.uniprot.org/uniprot/Q8IJT1_PLAF7 Q8IJT1_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.[RuleBase:RU004203] [[http://www.uniprot.org/uniprot/Q7K6A9_PLAF7 Q7K6A9_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.[RuleBase:RU004203] [[http://www.uniprot.org/uniprot/Q8IDG2_PLAF7 Q8IDG2_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.[RuleBase:RU000551] [[http://www.uniprot.org/uniprot/C0H4E8_PLAF7 C0H4E8_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.[RuleBase:RU004203] [[http://www.uniprot.org/uniprot/Q8IBI3_PLAF7 Q8IBI3_PLAF7]] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.[RuleBase:RU000551]
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Plasmodium falciparum 3d7]]
		+	[[Category: Proteasome endopeptidase complex]]
		+	[[Category: Gillett, D L]]
		+	[[Category: Griffin, M D.W]]
		+	[[Category: Hanssen, E]]
		+	[[Category: Leis, A P]]
		+	[[Category: Metcalfe, R D]]
	[[Category: Tilley, L]]		[[Category: Tilley, L]]
-	[[Category: ~~Griffin~~, ~~M.D.W~~]]	+	[[Category: Xie, S C]]
-	[[Category: ~~Xie, S.C~~]]	+	[[Category: 11s subunit]]
-	[[Category: ~~Gillett, D.L~~]]	+	[[Category: Complex]]
-	[[Category: ~~Hanssen, E~~]]	+	[[Category: Hydrolase]]
-	[[Category: ~~Leis, A.P~~]]	+	[[Category: Hydrolyse activator]]
		+	[[Category: Protease]]
		+	[[Category: Proteasome]]
		+	[[Category: Proteasome activator]]

OCA at 21:03, 5 June 2019

2019-06-05T21:03:07Z

←Older revision		Revision as of 21:03, 5 June 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6mux is ON HOLD	+	The entry 6mux is ON HOLD until Paper Publication

	Authors: Metcalfe, R.D., Xie, S.C., Hanssen, E., Gillett, D.L., Leis, A.P., Tilley, L., Griffin, M.D.W.		Authors: Metcalfe, R.D., Xie, S.C., Hanssen, E., Gillett, D.L., Leis, A.P., Tilley, L., Griffin, M.D.W.
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	Description: The structure of the Plasmodium falciparum 20S proteasome in complex with one PA28 activator		Description: The structure of the Plasmodium falciparum 20S proteasome in complex with one PA28 activator
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Hanssen, E]]
	[[Category: Metcalfe, R.D]]		[[Category: Metcalfe, R.D]]
	[[Category: Tilley, L]]		[[Category: Tilley, L]]
-	[[Category: Leis, A.P]]
	[[Category: Griffin, M.D.W]]		[[Category: Griffin, M.D.W]]
	[[Category: Xie, S.C]]		[[Category: Xie, S.C]]
	[[Category: Gillett, D.L]]		[[Category: Gillett, D.L]]
		+	[[Category: Hanssen, E]]
		+	[[Category: Leis, A.P]]

OCA at 04:50, 24 April 2019

2019-04-24T04:50:41Z

←Older revision		Revision as of 04:50, 24 April 2019
Line 5:		Line 5:
	Authors: Metcalfe, R.D., Xie, S.C., Hanssen, E., Gillett, D.L., Leis, A.P., Tilley, L., Griffin, M.D.W.		Authors: Metcalfe, R.D., Xie, S.C., Hanssen, E., Gillett, D.L., Leis, A.P., Tilley, L., Griffin, M.D.W.

-	Description: The structure of the Plasmodium falciparum 20S proteasome in complex with one PA28 activator.	+	Description: The structure of the Plasmodium falciparum 20S proteasome in complex with one PA28 activator
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Hanssen, E]]
	[[Category: Metcalfe, R.D]]		[[Category: Metcalfe, R.D]]
	[[Category: Tilley, L]]		[[Category: Tilley, L]]
		+	[[Category: Leis, A.P]]
	[[Category: Griffin, M.D.W]]		[[Category: Griffin, M.D.W]]
	[[Category: Xie, S.C]]		[[Category: Xie, S.C]]
	[[Category: Gillett, D.L]]		[[Category: Gillett, D.L]]
-	[[Category: Hanssen, E]]
-	[[Category: Leis, A.P]]

OCA at 04:05, 17 April 2019

2019-04-17T04:05:25Z

←Older revision		Revision as of 04:05, 17 April 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6mux is ON HOLD ~~until Paper Publication~~	+	The entry 6mux is ON HOLD

	Authors: Metcalfe, R.D., Xie, S.C., Hanssen, E., Gillett, D.L., Leis, A.P., Tilley, L., Griffin, M.D.W.		Authors: Metcalfe, R.D., Xie, S.C., Hanssen, E., Gillett, D.L., Leis, A.P., Tilley, L., Griffin, M.D.W.
Line 7:		Line 7:
	Description: The structure of the Plasmodium falciparum 20S proteasome in complex with one PA28 activator.		Description: The structure of the Plasmodium falciparum 20S proteasome in complex with one PA28 activator.
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Hanssen, E]]
	[[Category: Metcalfe, R.D]]		[[Category: Metcalfe, R.D]]
	[[Category: Tilley, L]]		[[Category: Tilley, L]]
-	[[Category: Leis, A.P]]
	[[Category: Griffin, M.D.W]]		[[Category: Griffin, M.D.W]]
	[[Category: Xie, S.C]]		[[Category: Xie, S.C]]
	[[Category: Gillett, D.L]]		[[Category: Gillett, D.L]]
		+	[[Category: Hanssen, E]]
		+	[[Category: Leis, A.P]]

OCA at 10:50, 7 November 2018

2018-11-07T10:50:38Z

←Older revision		Revision as of 10:50, 7 November 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6mux is ON HOLD	+	The entry 6mux is ON HOLD until Paper Publication

	Authors: Metcalfe, R.D., Xie, S.C., Hanssen, E., Gillett, D.L., Leis, A.P., Tilley, L., Griffin, M.D.W.		Authors: Metcalfe, R.D., Xie, S.C., Hanssen, E., Gillett, D.L., Leis, A.P., Tilley, L., Griffin, M.D.W.
Line 7:		Line 7:
	Description: The structure of the Plasmodium falciparum 20S proteasome in complex with one PA28 activator.		Description: The structure of the Plasmodium falciparum 20S proteasome in complex with one PA28 activator.
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Hanssen, E]]
	[[Category: Metcalfe, R.D]]		[[Category: Metcalfe, R.D]]
	[[Category: Tilley, L]]		[[Category: Tilley, L]]
		+	[[Category: Leis, A.P]]
	[[Category: Griffin, M.D.W]]		[[Category: Griffin, M.D.W]]
	[[Category: Xie, S.C]]		[[Category: Xie, S.C]]
	[[Category: Gillett, D.L]]		[[Category: Gillett, D.L]]
-	[[Category: Hanssen, E]]
-	[[Category: Leis, A.P]]