6mzv - Revision history

OCA at 14:45, 13 March 2024

2024-03-13T14:45:58Z

←Older revision		Revision as of 14:45, 13 March 2024
Line 3:		Line 3:
	<SX load='6mzv' size='340' side='right' viewer='molstar' caption='[[6mzv]], [[Resolution\|resolution]] 3.40Å' scene=''>		<SX load='6mzv' size='340' side='right' viewer='molstar' caption='[[6mzv]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mzv]] is a 42 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Halo1 Halo1~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MZV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6MZV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mzv]] is a 42 chain structure with sequence from [https://en.wikipedia.org/wiki/Haliangium_ochraceum_DSM_14365 Haliangium ochraceum DSM 14365]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MZV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MZV FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>Hoch_5816 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~502025 HALO1])~~, ~~Hoch_5815 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=502025 HALO1])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.4Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6mzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mzv OCA], [~~http~~://pdbe.org/6mzv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6mzv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6mzv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6mzv ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mzv OCA], [https://pdbe.org/6mzv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mzv RCSB], [https://www.ebi.ac.uk/pdbsum/6mzv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mzv ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/BMCT2_HALO1 BMCT2_HALO1] A minor component of the bacterial microcompartment (BMC) shell. Expression of 5 proteins in E.coli (BMC-H (Hoch_5815), BMC-P (Hoch_5814), and 3 BMC-T (Hoch_5812, Hoch_5816, Hoch_3341)) forms 40 nm artificial BMCs with a molecular mass of 6.5 MDa. One of 2 stacked pseudohexamers in the BMC. There are 20 BMC-T pseudohexamers per BMC, composed of mixed BMC-T1, BMC-T2 and BMC-T3. The shell facets are 20-30 Angstroms thick, with 1 of the stacked BMC-T trimers protruding to the exterior (PubMed:28642439, PubMed:30833088). The stacked trimers may serve as conduits to allow chemical flux across the protein shell, gated by Arg-70 which contacts Glu-69 in an adjacent subunit; they are flexible enough to play a role in accommodating variations in shell assembly (Probable).<ref>PMID:28642439</ref> <ref>PMID:30833088</ref> <ref>PMID:30833088</ref>
-	~~Bacterial microcompartments (BMCs) are composed of an enzymatic core encapsulated by a selectively permeable protein shell that enhances catalytic efficiency~~. Many pathogenic bacteria derive competitive advantages from their BMC-based catabolism, implicating BMCs as drug targets. BMC shells are of interest for bioengineering due to their diverse and selective permeability properties and because they self-assemble. A ~~complete understanding~~ of shell ~~composition and organization is a prerequisite for biotechnological applications~~. ~~Here, we report the cryoelectron microscopy structure~~ of a BMC ~~shell at 3.0~~-~~A resolution~~, ~~using an image-processing strategy that allowed us to determine the previously uncharacterized structural details of the interactions formed by the~~ BMC-T(S) and BMC-T(D) ~~shell subunits~~ in the ~~context of the assembled shell~~. ~~We found unexpected structural plasticity among these interactions~~, ~~resulting in distinct shell populations assembled from varying numbers~~ of ~~the~~ BMC-~~T(S)~~ and BMC-T(D) ~~subunits~~. ~~We discuss~~ the ~~implications of these findings on~~ shell ~~assembly and function.~~	+
-		+
-	~~The Plasticity of Molecular Interactions Governs Bacterial Microcompartment Shell Assembly.~~,~~Greber BJ, Sutter M, Kerfeld CA Structure. 2019 Feb 12. pii: S0969~~-~~2126~~(19)~~30017-6~~. ~~doi:, 10.1016/j.str.2019.01.017. PMID:30833088~~<ref>PMID:~~30833088~~</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.~~<br>	+
-	</~~div~~>	+
-	<~~div class="pdbe-citations 6mzv" style="background-color:#fffaf0;"~~></~~div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</SX>		</SX>
-	[[Category: ~~Halo1~~]]	+	[[Category: Haliangium ochraceum DSM 14365]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Greber~~, B J~~]]	+	[[Category: Greber BJ]]
-	[[Category: Kerfeld~~, C A~~]]	+	[[Category: Kerfeld CA]]
-	[[Category: Sutter, M]]	+	[[Category: Sutter M]]
-	~~[[Category: Bmc fold]]~~	+
-	~~[[Category: Compartmentalization]]~~	+
-	~~[[Category: Microcompartment]]~~	+
-	~~[[Category: Shell]]~~	+
-	~~[[Category: Structural protein~~]]	+

OCA at 03:02, 11 April 2020

2020-04-11T03:02:25Z

←Older revision		Revision as of 03:02, 11 April 2020
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	<SX load='6mzv' size='340' side='right' viewer='molstar' caption='[[6mzv]], [[Resolution\|resolution]] 3.40Å' scene=''>		<SX load='6mzv' size='340' side='right' viewer='molstar' caption='[[6mzv]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mzv]] is a 42 chain structure with sequence from [http://en.wikipedia.org/wiki/Halo1 Halo1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MZV OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6MZV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mzv]] is a 42 chain structure with sequence from [http://en.wikipedia.org/wiki/Halo1 Halo1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MZV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6MZV FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Hoch_5816 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=502025 HALO1]), Hoch_5815 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=502025 HALO1])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Hoch_5816 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=502025 HALO1]), Hoch_5815 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=502025 HALO1])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6mzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mzv OCA], [http://pdbe.org/6mzv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mzv RCSB], [http://www.ebi.ac.uk/pdbsum/6mzv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mzv ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6mzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mzv OCA], [http://pdbe.org/6mzv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mzv RCSB], [http://www.ebi.ac.uk/pdbsum/6mzv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mzv ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 22:40, 6 March 2020

2020-03-06T22:40:24Z

←Older revision		Revision as of 22:40, 6 March 2020
Line 1:		Line 1:

	==Cryo-EM structure of the HO BMC shell: BMC-TD focused structure, widened inner ring==		==Cryo-EM structure of the HO BMC shell: BMC-TD focused structure, widened inner ring==
-	<~~StructureSection~~ load='6mzv' size='340' side='right'caption='[[6mzv]], [[Resolution\|resolution]] 3.40Å' scene=''>	+	<SX load='6mzv' size='340' side='right' viewer='molstar' caption='[[6mzv]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6mzv]] is a 42 chain structure with sequence from [http://en.wikipedia.org/wiki/Halo1 Halo1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MZV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MZV FirstGlance]. <br>		<table><tr><td colspan='2'>[[6mzv]] is a 42 chain structure with sequence from [http://en.wikipedia.org/wiki/Halo1 Halo1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MZV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MZV FirstGlance]. <br>
Line 19:		Line 19:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Halo1]]		[[Category: Halo1]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 07:38, 23 May 2019

2019-05-23T07:38:24Z

←Older revision		Revision as of 07:38, 23 May 2019
Line 3:		Line 3:
	<StructureSection load='6mzv' size='340' side='right'caption='[[6mzv]], [[Resolution\|resolution]] 3.40Å' scene=''>		<StructureSection load='6mzv' size='340' side='right'caption='[[6mzv]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mzv]] is a 42 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MZV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MZV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mzv]] is a 42 chain structure with sequence from [http://en.wikipedia.org/wiki/Halo1 Halo1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MZV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MZV FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mzv OCA], [http://pdbe.org/6mzv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mzv RCSB], [http://www.ebi.ac.uk/pdbsum/6mzv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mzv ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Hoch_5816 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=502025 HALO1]), Hoch_5815 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=502025 HALO1])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mzv OCA], [http://pdbe.org/6mzv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mzv RCSB], [http://www.ebi.ac.uk/pdbsum/6mzv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mzv ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Halo1]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Greber, B J]]		[[Category: Greber, B J]]

OCA at 12:09, 13 March 2019

2019-03-13T12:09:06Z

←Older revision		Revision as of 12:09, 13 March 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6mzv is ~~ON HOLD until Paper~~ Publication	+	==Cryo-EM structure of the HO BMC shell: BMC-TD focused structure, widened inner ring==
		+	<StructureSection load='6mzv' size='340' side='right'caption='[[6mzv]], [[Resolution\|resolution]] 3.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6mzv]] is a 42 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MZV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MZV FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mzv OCA], [http://pdbe.org/6mzv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mzv RCSB], [http://www.ebi.ac.uk/pdbsum/6mzv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mzv ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial microcompartments (BMCs) are composed of an enzymatic core encapsulated by a selectively permeable protein shell that enhances catalytic efficiency. Many pathogenic bacteria derive competitive advantages from their BMC-based catabolism, implicating BMCs as drug targets. BMC shells are of interest for bioengineering due to their diverse and selective permeability properties and because they self-assemble. A complete understanding of shell composition and organization is a prerequisite for biotechnological applications. Here, we report the cryoelectron microscopy structure of a BMC shell at 3.0-A resolution, using an image-processing strategy that allowed us to determine the previously uncharacterized structural details of the interactions formed by the BMC-T(S) and BMC-T(D) shell subunits in the context of the assembled shell. We found unexpected structural plasticity among these interactions, resulting in distinct shell populations assembled from varying numbers of the BMC-T(S) and BMC-T(D) subunits. We discuss the implications of these findings on shell assembly and function.

-	~~Authors: Greber, B.J~~., ~~Sutter~~, M., Kerfeld, C.A.	+	The Plasticity of Molecular Interactions Governs Bacterial Microcompartment Shell Assembly.,Greber BJ, Sutter M, Kerfeld CA Structure. 2019 Feb 12. pii: S0969-2126(19)30017-6. doi:, 10.1016/j.str.2019.01.017. PMID:30833088<ref>PMID:30833088</ref>

-	~~Description: Cryo-EM structure~~ of the ~~HO BMC shell: BMC~~-~~TD focused structure, widened inner ring~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6mzv" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Greber, B J]]
		+	[[Category: Kerfeld, C A]]
	[[Category: Sutter, M]]		[[Category: Sutter, M]]
-	[[Category: ~~Greber, B.J~~]]	+	[[Category: Bmc fold]]
-	[[Category: ~~Kerfeld, C.A~~]]	+	[[Category: Compartmentalization]]
		+	[[Category: Microcompartment]]
		+	[[Category: Shell]]
		+	[[Category: Structural protein]]

OCA at 19:05, 2 December 2018

2018-12-02T19:05:22Z

←Older revision		Revision as of 19:05, 2 December 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6mzv is ON HOLD	+	The entry 6mzv is ON HOLD until Paper Publication

	Authors: Greber, B.J., Sutter, M., Kerfeld, C.A.		Authors: Greber, B.J., Sutter, M., Kerfeld, C.A.

OCA: Protected "6mzv" [edit=sysop:move=sysop]

2018-11-14T06:43:50Z

Protected "6mzv" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:43, 14 November 2018

OCA: New page: '''Unreleased structure''' The entry 6mzv is ON HOLD Authors: Greber, B.J., Sutter, M., Kerfeld, C.A. Description: Cryo-EM structure of the HO BMC shell: BMC-TD focused structure, wide...

2018-11-14T06:43:48Z

New page: '''Unreleased structure''' The entry 6mzv is ON HOLD Authors: Greber, B.J., Sutter, M., Kerfeld, C.A. Description: Cryo-EM structure of the HO BMC shell: BMC-TD focused structure, wide...

New page

'''Unreleased structure'''

The entry 6mzv is ON HOLD

Authors: Greber, B.J., Sutter, M., Kerfeld, C.A.

Description: Cryo-EM structure of the HO BMC shell: BMC-TD focused structure, widened inner ring
[[Category: Unreleased Structures]]
[[Category: Sutter, M]]
[[Category: Greber, B.J]]
[[Category: Kerfeld, C.A]]