6n5j - Revision history

OCA at 14:46, 13 March 2024

2024-03-13T14:46:39Z

←Older revision		Revision as of 14:46, 13 March 2024
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	<StructureSection load='6n5j' size='340' side='right'caption='[[6n5j]], [[Resolution\|resolution]] 1.37Å' scene=''>		<StructureSection load='6n5j' size='340' side='right'caption='[[6n5j]], [[Resolution\|resolution]] 1.37Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6n5j]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N5J OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6N5J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6n5j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N5J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6N5J FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=ACT:ACETATE+ION'>ACT</scene~~>, ~~<scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.37Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~ftsY, b3464, JW3429 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=83333 ECOLI])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6n5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n5j OCA], [~~http~~://pdbe.org/6n5j PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6n5j RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6n5j PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6n5j ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6n5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n5j OCA], [https://pdbe.org/6n5j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6n5j RCSB], [https://www.ebi.ac.uk/pdbsum/6n5j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6n5j ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/FTSY_ECOLI FTSY_ECOLI]] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC). Interaction with SRP-RNC leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components.<ref>PMID:8194520</ref> <ref>PMID:9305630</ref> <ref>PMID:11735405</ref> <ref>PMID:11741850</ref> <ref>PMID:15148364</ref> <ref>PMID:17682051</ref>	+	[https://www.uniprot.org/uniprot/FTSY_ECOLI FTSY_ECOLI] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC). Interaction with SRP-RNC leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components.<ref>PMID:8194520</ref> <ref>PMID:9305630</ref> <ref>PMID:11735405</ref> <ref>PMID:11741850</ref> <ref>PMID:15148364</ref> <ref>PMID:17682051</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The bacterial signal recognition particle (SRP) receptor, FtsY, participates with the SRP in co-translation targeting of proteins. Multiple crystal structures of the NG domain of E. coli FtsYNG have been determined at high-resolution (1.22-1.88A), in the nucleotide-free (apo) form as well as bound to GDP and non-hydrolysable GTP analogues. The combination of high-resolution and multiple solved structures of FtsYNG in different states revealed a new sensor-relay system of this unique GTPase receptor. A nucleotide sensing function of the P-loop assists FtsYNG in nucleotide-binding and contributes to modulate nucleotide binding properties in SRP GTPases. A reorganization of the other G-loops and the insertion binding domain (IBD) is observed only upon transition from a diphosphate to a triphosphate nucleotide. The role of a magnesium ion during the GDP and GTP-bound states has also been observed. The binding of magnesium in the nucleotide site causes the reorientation of the beta- and gamma- phosphate groups toward the jaws of the P-loop and stabilizes the binding of the nucleotide, creating a network of hydrogen and water-bridge interactions.	+
-		+
-	Structural insights into the G-loop dynamics of E. coli FtsY NG domain.,Faoro C, Ataide SF J Struct Biol. 2019 Sep 11. pii: S1047-8477(19)30198-4. doi:, 10.1016/j.jsb.2019.09.004. PMID:31520694<ref>PMID:31520694</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6n5j" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
-	*[[Signal recognition particle receptor\|Signal recognition particle receptor]]	+	*[[Signal recognition particle receptor 3D structures\|Signal recognition particle receptor 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ataide~~, S F~~]]	+	[[Category: Ataide SF]]
-	[[Category: Faoro, C]]	+	[[Category: Faoro C]]
-	~~[[Category: Ftsy]]~~	+
-	~~[[Category: Signal recognition particle receptor]]~~	+
-	~~[[Category: Sr]]~~	+
-	~~[[Category: Srp]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 10:20, 18 March 2020

2020-03-18T10:20:43Z

←Older revision		Revision as of 10:20, 18 March 2020
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	</div>		</div>
	<div class="pdbe-citations 6n5j" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6n5j" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Signal recognition particle receptor\|Signal recognition particle receptor]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:29, 4 December 2019

2019-12-04T10:29:14Z

←Older revision		Revision as of 10:29, 4 December 2019
Line 3:		Line 3:
	<StructureSection load='6n5j' size='340' side='right'caption='[[6n5j]], [[Resolution\|resolution]] 1.37Å' scene=''>		<StructureSection load='6n5j' size='340' side='right'caption='[[6n5j]], [[Resolution\|resolution]] 1.37Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6n5j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N5J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N5J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6n5j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N5J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N5J FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ftsY, b3464, JW3429 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n5j OCA], [http://pdbe.org/6n5j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n5j RCSB], [http://www.ebi.ac.uk/pdbsum/6n5j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n5j ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n5j OCA], [http://pdbe.org/6n5j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n5j RCSB], [http://www.ebi.ac.uk/pdbsum/6n5j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n5j ProSAT]</span></td></tr>
	</table>		</table>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ecoli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Ataide, S F]]		[[Category: Ataide, S F]]

OCA at 05:20, 10 October 2019

2019-10-10T05:20:24Z

←Older revision		Revision as of 05:20, 10 October 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6n5j is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==FtsY-NG high-resolution==
		+	<StructureSection load='6n5j' size='340' side='right'caption='[[6n5j]], [[Resolution\|resolution]] 1.37Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6n5j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N5J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N5J FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n5j OCA], [http://pdbe.org/6n5j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n5j RCSB], [http://www.ebi.ac.uk/pdbsum/6n5j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n5j ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FTSY_ECOLI FTSY_ECOLI]] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC). Interaction with SRP-RNC leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components.<ref>PMID:8194520</ref> <ref>PMID:9305630</ref> <ref>PMID:11735405</ref> <ref>PMID:11741850</ref> <ref>PMID:15148364</ref> <ref>PMID:17682051</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The bacterial signal recognition particle (SRP) receptor, FtsY, participates with the SRP in co-translation targeting of proteins. Multiple crystal structures of the NG domain of E. coli FtsYNG have been determined at high-resolution (1.22-1.88A), in the nucleotide-free (apo) form as well as bound to GDP and non-hydrolysable GTP analogues. The combination of high-resolution and multiple solved structures of FtsYNG in different states revealed a new sensor-relay system of this unique GTPase receptor. A nucleotide sensing function of the P-loop assists FtsYNG in nucleotide-binding and contributes to modulate nucleotide binding properties in SRP GTPases. A reorganization of the other G-loops and the insertion binding domain (IBD) is observed only upon transition from a diphosphate to a triphosphate nucleotide. The role of a magnesium ion during the GDP and GTP-bound states has also been observed. The binding of magnesium in the nucleotide site causes the reorientation of the beta- and gamma- phosphate groups toward the jaws of the P-loop and stabilizes the binding of the nucleotide, creating a network of hydrogen and water-bridge interactions.

-	~~Authors: Ataide, S~~.F., Faoro, C.	+	Structural insights into the G-loop dynamics of E. coli FtsY NG domain.,Faoro C, Ataide SF J Struct Biol. 2019 Sep 11. pii: S1047-8477(19)30198-4. doi:, 10.1016/j.jsb.2019.09.004. PMID:31520694<ref>PMID:31520694</ref>

-	~~Description: FtsY~~-~~NG high~~-~~resolution~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Ataide, S.F]]	+	<div class="pdbe-citations 6n5j" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Ataide, S F]]
	[[Category: Faoro, C]]		[[Category: Faoro, C]]
		+	[[Category: Ftsy]]
		+	[[Category: Signal recognition particle receptor]]
		+	[[Category: Sr]]
		+	[[Category: Srp]]
		+	[[Category: Transport protein]]

OCA at 10:33, 16 January 2019

2019-01-16T10:33:19Z

←Older revision		Revision as of 10:33, 16 January 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6n5j is ON HOLD	+	The entry 6n5j is ON HOLD until Paper Publication

	Authors: Ataide, S.F., Faoro, C.		Authors: Ataide, S.F., Faoro, C.

OCA: Protected "6n5j" [edit=sysop:move=sysop]

2018-12-02T19:18:52Z

Protected "6n5j" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:18, 2 December 2018

OCA: New page: '''Unreleased structure''' The entry 6n5j is ON HOLD Authors: Ataide, S.F., Faoro, C. Description: FtsY-NG high-resolution Category: Unreleased Structures Category: Ataide, S.F...

2018-12-02T19:18:50Z

New page: '''Unreleased structure''' The entry 6n5j is ON HOLD Authors: Ataide, S.F., Faoro, C. Description: FtsY-NG high-resolution Category: Unreleased Structures Category: Ataide, S.F...

New page

'''Unreleased structure'''

The entry 6n5j is ON HOLD

Authors: Ataide, S.F., Faoro, C.

Description: FtsY-NG high-resolution
[[Category: Unreleased Structures]]
[[Category: Ataide, S.F]]
[[Category: Faoro, C]]