6nm5 - Revision history

OCA at 09:21, 20 March 2024

2024-03-20T09:21:23Z

←Older revision		Revision as of 09:21, 20 March 2024
Line 3:		Line 3:
	<SX load='6nm5' size='340' side='right' viewer='molstar' caption='[[6nm5]], [[Resolution\|resolution]] 6.20Å' scene=''>		<SX load='6nm5' size='340' side='right' viewer='molstar' caption='[[6nm5]], [[Resolution\|resolution]] 6.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6nm5]] is a 76 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Enterobacteria_phage_ms2 Enterobacteria phage ms2~~] and [~~http~~://en.wikipedia.org/wiki/~~Escherichia_coli~~ Escherichia ~~coli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NM5 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6NM5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6nm5]] is a 76 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_virus_MS2 Escherichia virus MS2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NM5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NM5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KSV:(2R)-2,3-dihydroxypropyl+ethyl+hydrogen+(S)-phosphate'>KSV</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 6.2Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6nm5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nm5 OCA], [~~http~~://pdbe.org/6nm5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6nm5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6nm5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6nm5 ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KSV:(2R)-2,3-dihydroxypropyl+ethyl+hydrogen+(S)-phosphate'>KSV</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nm5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nm5 OCA], [https://pdbe.org/6nm5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nm5 RCSB], [https://www.ebi.ac.uk/pdbsum/6nm5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nm5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~MAT_BPMS2 MAT_BPMS2]~~] The maturation protein is required for the typical attachment of the phage to the side of the bacterial pili (PubMed:23810697). ~~MP binds~~ to sequences located toward each end of the genome, hence circularizing it (PubMed:26608810). The RNA genome-MP complex is released from the capsid upon host receptor binding (PubMed:23810697). MP enters the cell along with the viral RNA (PubMed:4551992).<ref>PMID:23810697</ref> <ref>PMID:26608810</ref> <ref>PMID:4551992</ref>	+	[https://www.uniprot.org/uniprot/MATA_BPMS2 MATA_BPMS2] The maturation protein is required for the typical attachment of the phage to the side of the bacterial pili (PubMed:23810697). Binds to sequences located toward each end of the genome, hence circularizing it (PubMed:26608810). The RNA genome-maturation protein A complex is released from the capsid upon host receptor binding (PubMed:23810697). Maturation protein A enters the cell along with the viral RNA (PubMed:4551992).<ref>PMID:23810697</ref> <ref>PMID:26608810</ref> <ref>PMID:4551992</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Single-stranded RNA bacteriophages (ssRNA phages) infect Gram-negative bacteria via a single maturation protein (Mat), which attaches to a retractile pilus of the host. Here we present structures of the ssRNA phage MS2 in complex with the Escherichia coli F-pilus, showing a network of hydrophobic and electrostatic interactions at the Mat-pilus interface. Moreover, binding of the pilus induces slight orientational variations of the Mat relative to the rest of the phage capsid, priming the Mat-connected genomic RNA (gRNA) for its release from the virions. The exposed tip of the attached Mat points opposite to the direction of the pilus retraction, which may facilitate the translocation of the gRNA from the capsid into the host cytosol. In addition, our structures determine the orientation of the assembled F-pilin subunits relative to the cell envelope, providing insights into the F-like type IV secretion systems.	+
-		+
-	Structural basis for the adsorption of a single-stranded RNA bacteriophage.,Meng R, Jiang M, Cui Z, Chang JY, Yang K, Jakana J, Yu X, Wang Z, Hu B, Zhang J Nat Commun. 2019 Jul 16;10(1):3130. doi: 10.1038/s41467-019-11126-8. PMID:31311931<ref>PMID:31311931</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6nm5" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</SX>		</SX>
-	[[Category: Enterobacteria phage ms2]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
		+	[[Category: Escherichia virus MS2]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chang, J]]	+	[[Category: Chang J]]
-	[[Category: Meng, R]]	+	[[Category: Meng R]]
-	[[Category: Zhang, J]]	+	[[Category: Zhang J]]
-	~~[[Category: Adsorption complex]]~~	+
-	~~[[Category: F-pilus]]~~	+
-	~~[[Category: Ms2 maturation protein]]~~	+
-	~~[[Category: Protein binding~~]]	+

OCA at 03:26, 11 April 2020

2020-04-11T03:26:06Z

←Older revision		Revision as of 03:26, 11 April 2020
Line 3:		Line 3:
	<SX load='6nm5' size='340' side='right' viewer='molstar' caption='[[6nm5]], [[Resolution\|resolution]] 6.20Å' scene=''>		<SX load='6nm5' size='340' side='right' viewer='molstar' caption='[[6nm5]], [[Resolution\|resolution]] 6.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6nm5]] is a 76 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_ms2 Enterobacteria phage ms2] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NM5 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6NM5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6nm5]] is a 76 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_ms2 Enterobacteria phage ms2] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NM5 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6NM5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=KSV:(2R)-2,3-dihydroxypropyl+ethyl+hydrogen+(S)-phosphate'>KSV</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KSV:(2R)-2,3-dihydroxypropyl+ethyl+hydrogen+(S)-phosphate'>KSV</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6nm5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nm5 OCA], [http://pdbe.org/6nm5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nm5 RCSB], [http://www.ebi.ac.uk/pdbsum/6nm5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nm5 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6nm5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nm5 OCA], [http://pdbe.org/6nm5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nm5 RCSB], [http://www.ebi.ac.uk/pdbsum/6nm5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nm5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 22:52, 6 March 2020

2020-03-06T22:52:32Z

←Older revision		Revision as of 22:52, 6 March 2020
Line 1:		Line 1:

	==F-pilus/MS2 Maturation protein complex==		==F-pilus/MS2 Maturation protein complex==
-	<~~StructureSection~~ load='6nm5' size='340' side='right'caption='[[6nm5]], [[Resolution\|resolution]] 6.20Å' scene=''>	+	<SX load='6nm5' size='340' side='right' viewer='molstar' caption='[[6nm5]], [[Resolution\|resolution]] 6.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6nm5]] is a 76 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_ms2 Enterobacteria phage ms2] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NM5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NM5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6nm5]] is a 76 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_ms2 Enterobacteria phage ms2] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NM5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NM5 FirstGlance]. <br>
Line 21:		Line 21:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Enterobacteria phage ms2]]		[[Category: Enterobacteria phage ms2]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]

OCA at 07:00, 31 July 2019

2019-07-31T07:00:26Z

←Older revision		Revision as of 07:00, 31 July 2019
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/MAT_BPMS2 MAT_BPMS2]] The maturation protein is required for the typical attachment of the phage to the side of the bacterial pili (PubMed:23810697). MP binds to sequences located toward each end of the genome, hence circularizing it (PubMed:26608810). The RNA genome-MP complex is released from the capsid upon host receptor binding (PubMed:23810697). MP enters the cell along with the viral RNA (PubMed:4551992).<ref>PMID:23810697</ref> <ref>PMID:26608810</ref> <ref>PMID:4551992</ref>		[[http://www.uniprot.org/uniprot/MAT_BPMS2 MAT_BPMS2]] The maturation protein is required for the typical attachment of the phage to the side of the bacterial pili (PubMed:23810697). MP binds to sequences located toward each end of the genome, hence circularizing it (PubMed:26608810). The RNA genome-MP complex is released from the capsid upon host receptor binding (PubMed:23810697). MP enters the cell along with the viral RNA (PubMed:4551992).<ref>PMID:23810697</ref> <ref>PMID:26608810</ref> <ref>PMID:4551992</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Single-stranded RNA bacteriophages (ssRNA phages) infect Gram-negative bacteria via a single maturation protein (Mat), which attaches to a retractile pilus of the host. Here we present structures of the ssRNA phage MS2 in complex with the Escherichia coli F-pilus, showing a network of hydrophobic and electrostatic interactions at the Mat-pilus interface. Moreover, binding of the pilus induces slight orientational variations of the Mat relative to the rest of the phage capsid, priming the Mat-connected genomic RNA (gRNA) for its release from the virions. The exposed tip of the attached Mat points opposite to the direction of the pilus retraction, which may facilitate the translocation of the gRNA from the capsid into the host cytosol. In addition, our structures determine the orientation of the assembled F-pilin subunits relative to the cell envelope, providing insights into the F-like type IV secretion systems.
		+
		+	Structural basis for the adsorption of a single-stranded RNA bacteriophage.,Meng R, Jiang M, Cui Z, Chang JY, Yang K, Jakana J, Yu X, Wang Z, Hu B, Zhang J Nat Commun. 2019 Jul 16;10(1):3130. doi: 10.1038/s41467-019-11126-8. PMID:31311931<ref>PMID:31311931</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6nm5" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:17, 24 July 2019

2019-07-24T06:17:33Z

←Older revision		Revision as of 06:17, 24 July 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6nm5 is ~~ON HOLD until Paper Publication~~	+	==F-pilus/MS2 Maturation protein complex==
-		+	<StructureSection load='6nm5' size='340' side='right'caption='[[6nm5]], [[Resolution\|resolution]] 6.20Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6nm5]] is a 76 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_ms2 Enterobacteria phage ms2] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NM5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NM5 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=KSV:(2R)-2,3-dihydroxypropyl+ethyl+hydrogen+(S)-phosphate'>KSV</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nm5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nm5 OCA], [http://pdbe.org/6nm5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nm5 RCSB], [http://www.ebi.ac.uk/pdbsum/6nm5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nm5 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MAT_BPMS2 MAT_BPMS2]] The maturation protein is required for the typical attachment of the phage to the side of the bacterial pili (PubMed:23810697). MP binds to sequences located toward each end of the genome, hence circularizing it (PubMed:26608810). The RNA genome-MP complex is released from the capsid upon host receptor binding (PubMed:23810697). MP enters the cell along with the viral RNA (PubMed:4551992).<ref>PMID:23810697</ref> <ref>PMID:26608810</ref> <ref>PMID:4551992</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Enterobacteria phage ms2]]
		+	[[Category: Escherichia coli]]
		+	[[Category: Large Structures]]
		+	[[Category: Chang, J]]
		+	[[Category: Meng, R]]
		+	[[Category: Zhang, J]]
		+	[[Category: Adsorption complex]]
		+	[[Category: F-pilus]]
		+	[[Category: Ms2 maturation protein]]
		+	[[Category: Protein binding]]

OCA at 07:34, 13 February 2019

2019-02-13T07:34:26Z

←Older revision		Revision as of 07:34, 13 February 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6nm5 is ON HOLD	+	The entry 6nm5 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6nm5" [edit=sysop:move=sysop]

2019-01-16T10:46:08Z

Protected "6nm5" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:46, 16 January 2019

OCA: New page: '''Unreleased structure''' The entry 6nm5 is ON HOLD Authors: Description: Category: Unreleased Structures

2019-01-16T10:46:07Z

New page: '''Unreleased structure''' The entry 6nm5 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6nm5 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]